Endothelial Nitric Oxyde Synthase Gene Polymorphisms in a Tunisian Deep Vein Thrombosis Group  

作　　者：Nedra Grira Nadia Ben Abdelhafidh Manel Ayoub Rihab Sendesni Bochra Adib Najla Stambouli Salah Othmani Zied Aouni Chakib Mazigh Nedra Grira;Nadia Ben Abdelhafidh;Manel Ayoub;Rihab Sendesni;Bochra Adib;Najla Stambouli;Salah Othmani;Zied Aouni;Chakib Mazigh(Research Unit, Biochemistry Department, Military Hospital of Tunis, Montfleury, Tunisia;Internal Medicine Department, Military Hospital of Tunis, Montfleury, Tunisia)

机构地区：[1]Research Unit, Biochemistry Department, Military Hospital of Tunis, Montfleury, Tunisia [2]Internal Medicine Department, Military Hospital of Tunis, Montfleury, Tunisia

出　　处：《Journal of Biosciences and Medicines》2016年第9期33-41,共9页生物科学与医学（英文）

摘　　要：Deep vein thrombosis (DVT) is a multi-factorial disease involving both genetic and acquired risk factors. The objective of this study was to determine the frequencies of endothelial nitric oxide synthase (eNOS) gene polymorphisms G894T (rs1799983) and T-786C (rs2070744) to assess the role of these polymorphisms as a potential risk factor in the development of DVT. Methods: In this case-control study, we included 32 patients with deep vein thrombosis (DVT) and 31 healthy control subjects. Clinical characteristics were collected. Lipids plasma concentrations were determined by the colorimetric method. Genotyping for the polymorphisms was performed by restriction fragment length polymorphism (PCR-RFLP) method. Results: We had found that the eNos G894T genotype G/T was significantly increasing the risk of DTV (P = 0.042, OR = 3.9;95% CI = 1.09 to 13.92). But no association of the eNOS T-786C variant and DVT was found. For the eNOs T-786C polymorphism, the frequency of the T/T genotype was 87.5% in patients (with an allelic frequency of T Allele equal to 91%). No significant difference was noted between the two groups (P > 0.05). Conclusion: The eNOs G894T polymorphism seems to be in association with DVT and may be considered as a risk factor, but this is not the case for the T-786C polymorphism.Deep vein thrombosis (DVT) is a multi-factorial disease involving both genetic and acquired risk factors. The objective of this study was to determine the frequencies of endothelial nitric oxide synthase (eNOS) gene polymorphisms G894T (rs1799983) and T-786C (rs2070744) to assess the role of these polymorphisms as a potential risk factor in the development of DVT. Methods: In this case-control study, we included 32 patients with deep vein thrombosis (DVT) and 31 healthy control subjects. Clinical characteristics were collected. Lipids plasma concentrations were determined by the colorimetric method. Genotyping for the polymorphisms was performed by restriction fragment length polymorphism (PCR-RFLP) method. Results: We had found that the eNos G894T genotype G/T was significantly increasing the risk of DTV (P = 0.042, OR = 3.9;95% CI = 1.09 to 13.92). But no association of the eNOS T-786C variant and DVT was found. For the eNOs T-786C polymorphism, the frequency of the T/T genotype was 87.5% in patients (with an allelic frequency of T Allele equal to 91%). No significant difference was noted between the two groups (P > 0.05). Conclusion: The eNOs G894T polymorphism seems to be in association with DVT and may be considered as a risk factor, but this is not the case for the T-786C polymorphism.

关 键 词：Deep Vein Hrombosis ENOS NO G894T Polymorphism T-786C Polymorphism 

分 类 号：R54[医药卫生—心血管疾病]