Anther Culture and Plant Regeneration of Tetraploid Purple Coneflower (Echinacea purpurea L.)  

作　　者：Xiaolu Chen Dahanayake Nilanthi Yuesheng Yang Hong Wu Xiaolu Chen;Dahanayake Nilanthi;Yuesheng Yang;Hong Wu(Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture, Danzhou, China;College of Life Science, South China Agricultural University, Guangzhou, China;Department of Agricultural Biology, Faculty of Agriculture, University of Ruhuna, Matara, Sri Lanka)

机构地区：[1]Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture, Danzhou, China [2]College of Life Science, South China Agricultural University, Guangzhou, China [3]Department of Agricultural Biology, Faculty of Agriculture, University of Ruhuna, Matara, Sri Lanka

出　　处：《Journal of Biosciences and Medicines》2016年第12期89-96,共8页生物科学与医学（英文）

摘　　要：Anthersisolated from tetraploid purple coneflowerplants were cultured in vitro. The highest callus induction rate was obtained when the medium was consisted of N6 basal elements, 4% sucrose, 0.5 mg?L?1 BA, and 0.10 mg?L?1 NAA. Various morphogenesis such as globular, heart-shape, torpedo-shapeand final state embryos as well asvarious texture calluses around were observed. Out of 110 plantlets regenerated, 104 were confirmed as diploid and the rest were as tetraploid. Plants of one diploid offspring strain presented aspecialcharacter in pot: unlike the original tetraploid plants, it grown tubular, bisexual ray florets. The results obtained in the present studies indicated that although the tetraploid purple coneflower plants produced only diploid microspores, the recovery of some useful mutants through in vitro anther cultures might be reasonably expected.Anthersisolated from tetraploid purple coneflowerplants were cultured in vitro. The highest callus induction rate was obtained when the medium was consisted of N6 basal elements, 4% sucrose, 0.5 mg?L?1 BA, and 0.10 mg?L?1 NAA. Various morphogenesis such as globular, heart-shape, torpedo-shapeand final state embryos as well asvarious texture calluses around were observed. Out of 110 plantlets regenerated, 104 were confirmed as diploid and the rest were as tetraploid. Plants of one diploid offspring strain presented aspecialcharacter in pot: unlike the original tetraploid plants, it grown tubular, bisexual ray florets. The results obtained in the present studies indicated that although the tetraploid purple coneflower plants produced only diploid microspores, the recovery of some useful mutants through in vitro anther cultures might be reasonably expected.

关 键 词：Purple Coneflower Echinacea purpurea Anther Culture TETRAPLOID 

分 类 号：Q94[生物学—植物学]