Occult Hepatitis B Virus Infection: A Killer Hidden in Transfused Blood, Sudan  

作　　者：Elhadi Abdalla Ahmed Abdelrahman Eldaw Mohammed Mubarak Ahmed Elshafia Bakri Yousif Mohamed Nour Elhadi Abdalla Ahmed;Abdelrahman Eldaw Mohammed;Mubarak Ahmed Elshafia;Bakri Yousif Mohamed Nour(Department of Medical Microbiology, Faculty of Medical Laboratory Sciences, University of Gezira, Wad Medani, Sudan;Department of Haematology & Immunohaematology, Faculty of Medical Laboratory Sciences, University of Gezira, Wad Medani, Sudan;Department of Medical Parasitology, Faculty of Medical Laboratory Sciences, University of Gezira, Wad Medani,, Sudan)

机构地区：[1]Department of Medical Microbiology, Faculty of Medical Laboratory Sciences, University of Gezira, Wad Medani, Sudan [2]Department of Haematology & Immunohaematology, Faculty of Medical Laboratory Sciences, University of Gezira, Wad Medani, Sudan [3]Department of Medical Parasitology, Faculty of Medical Laboratory Sciences, University of Gezira, Wad Medani,, Sudan

出　　处：《Journal of Biosciences and Medicines》2022年第10期173-181,共9页生物科学与医学（英文）

摘　　要：Introduction: Transmission of hepatitis B virus (HBV) through the blood is a significant risk, especially in poor countries with high endemicity. Occult transmission of HBV (OBI) is an important acquisition scenario. Objective: A cross-sectional laboratory-based study followed to detect OBI in blood of accepted donors admitted to Mad Medani Blood Bank, Sudan. Methods: During the study, 200 accepted blood units were examined for HBsAg by ELISA technique and negative samples were tested for total anti-HBc antibodies using electroluminescence immunoassay (ECLIAS). HBV DNA amplification was performed for units that showed total anti-Hbc positivity. Results: Of the 200 blood units, 3 appeared positive by ELISA. Total anti-HBc antibodies were present in 34% (67/197) of blood units. HBV DNA was successfully amplified in 52.2% (35/67) of total anti-HBc positive samples. A significant association was observed between reactive total anti-HBc and age group (p Conclusion: OBI was recorded at a high rate in the blood of donors, which necessitates the implementation of detection methods to protect the recipients.Introduction: Transmission of hepatitis B virus (HBV) through the blood is a significant risk, especially in poor countries with high endemicity. Occult transmission of HBV (OBI) is an important acquisition scenario. Objective: A cross-sectional laboratory-based study followed to detect OBI in blood of accepted donors admitted to Mad Medani Blood Bank, Sudan. Methods: During the study, 200 accepted blood units were examined for HBsAg by ELISA technique and negative samples were tested for total anti-HBc antibodies using electroluminescence immunoassay (ECLIAS). HBV DNA amplification was performed for units that showed total anti-Hbc positivity. Results: Of the 200 blood units, 3 appeared positive by ELISA. Total anti-HBc antibodies were present in 34% (67/197) of blood units. HBV DNA was successfully amplified in 52.2% (35/67) of total anti-HBc positive samples. A significant association was observed between reactive total anti-HBc and age group (p Conclusion: OBI was recorded at a high rate in the blood of donors, which necessitates the implementation of detection methods to protect the recipients.

关 键 词：OBI Blood Donors NESTED-PCR SUDAN 

分 类 号：R51[医药卫生—内科学]