Antioxidants Enzymes Activities and NO Levels in Hemodialysis and Continuous Ambulatory Peritoneal Dialysis—A Comparative Study  

作　　者：Natalia Cristina Aparecida Ribeiro e Ribeiro Anderson Martins Tavares Jaslana Hainfellner da Silva João Paulo Lima Daher Salim Kanaan Luciene de Carvalho Cardoso-Weide Natalia Cristina Aparecida Ribeiro e Ribeiro;Anderson Martins Tavares;Jaslana Hainfellner da Silva;João Paulo Lima Daher;Salim Kanaan;Luciene de Carvalho Cardoso-Weide(Laboratory of Neuroplasticity, Neurobiology Department, Biology Institute, Federal Fluminense University, Rio de Janeiro, Brazil;Postgraduate Program in Medical Sciences, School of Medicine, Federal Fluminense University, Rio de Janeiro, Brazil;School of Biology, UNIVERSO—Salgado de Oliveira University, Rio de Janeiro, Brazil;Department of Pathology, Faculty of Medicine, Federal Fluminense University, Rio de Janeiro, Brazil)

机构地区：[1]Laboratory of Neuroplasticity, Neurobiology Department, Biology Institute, Federal Fluminense University, Rio de Janeiro, Brazil [2]Postgraduate Program in Medical Sciences, School of Medicine, Federal Fluminense University, Rio de Janeiro, Brazil [3]School of Biology, UNIVERSO—Salgado de Oliveira University, Rio de Janeiro, Brazil [4]Department of Pathology, Faculty of Medicine, Federal Fluminense University, Rio de Janeiro, Brazil

出　　处：《Journal of Biosciences and Medicines》2024年第2期50-59,共10页生物科学与医学（英文）

摘　　要：It consists of a retrospective study with twenty-two individuals diagnosed with DRC: fourteen HD and eight CAPD, followed up in the dialysis center of Antonio Pedro University Hospital (HUAP) of Fluminense Federal University (UFF), between 2015 and 2017. Fifteen healthy control (HC) subjects were included, without diagnosed CKD. Patients with HIV positive, hepatitis A, B, and C, pregnant, cancer, smokers, alcoholics and those exposed to X-rays in the last 3 months, were excluded. Objectives: As oxidative stress and endothelial dysfunction may be linked to the higher prevalence of CVD in CKD patients, we measured the activities of the antioxidants enzymes: SOD and GPx and total NO levels in the plasma and serum of end-stage CKD patients undergoing dialysis therapy, comparing with the HC group. Methods: Quantification of NO levels was performed by fluorometric kit, while activities of SOD and GPx were determined using kinetic methods. Results: We found higher plasma SOD activity in HD (8.58 U/ml) and CAPD (10.14 U/ml), compared to C (3.73 U/ml) group, while GPx activity was decreased in HD (115.38 nmol/h/ml) and CAPD (122.76 nmol/h/ml) groups compared to HC group (275.83 nmol/h/ml). Total serum NO concentration was decreased in HD (14.09 pmol/μl) and CAPD (10.26 pmol/μl), compared to non-CKD patients (49.65 pmol/μl). Conclusion: Decreased total serum NO and GPx activities may lead to endothelial dysfunction and consequently a higher prevalence of CVD in CKD patients.It consists of a retrospective study with twenty-two individuals diagnosed with DRC: fourteen HD and eight CAPD, followed up in the dialysis center of Antonio Pedro University Hospital (HUAP) of Fluminense Federal University (UFF), between 2015 and 2017. Fifteen healthy control (HC) subjects were included, without diagnosed CKD. Patients with HIV positive, hepatitis A, B, and C, pregnant, cancer, smokers, alcoholics and those exposed to X-rays in the last 3 months, were excluded. Objectives: As oxidative stress and endothelial dysfunction may be linked to the higher prevalence of CVD in CKD patients, we measured the activities of the antioxidants enzymes: SOD and GPx and total NO levels in the plasma and serum of end-stage CKD patients undergoing dialysis therapy, comparing with the HC group. Methods: Quantification of NO levels was performed by fluorometric kit, while activities of SOD and GPx were determined using kinetic methods. Results: We found higher plasma SOD activity in HD (8.58 U/ml) and CAPD (10.14 U/ml), compared to C (3.73 U/ml) group, while GPx activity was decreased in HD (115.38 nmol/h/ml) and CAPD (122.76 nmol/h/ml) groups compared to HC group (275.83 nmol/h/ml). Total serum NO concentration was decreased in HD (14.09 pmol/μl) and CAPD (10.26 pmol/μl), compared to non-CKD patients (49.65 pmol/μl). Conclusion: Decreased total serum NO and GPx activities may lead to endothelial dysfunction and consequently a higher prevalence of CVD in CKD patients.

关 键 词：HEMODIALYSIS CAPD SOD GPX NO 

分 类 号：R69[医药卫生—泌尿科学]