Molecular Detection of Extended-Spectrum Beta Lactamase and Carbapenemase Genes in Escherichia coli and Salmonella spp. Isolated from Chickens Consumed Outdoors in Ouagadougou, Burkina Faso  

作　　者：Amana Mètuor Dabiré Stéphanie Lynseh Carine Sita Bénao Nicolas Ouédraogo Rahimatou Yasmine Wendkouni Tiemtoré Rhaina Olivia Badini Lionel Eliada Benoit Bambara Jacques Simporé Amana Mètuor Dabiré;Stéphanie Lynseh Carine Sita Bénao;Nicolas Ouédraogo;Rahimatou Yasmine Wendkouni Tiemtoré;Rhaina Olivia Badini;Lionel Eliada Benoit Bambara;Jacques Simporé(Biomolecular and Genetic Laboratory (LABIOGENE), Pietro Annigoni Biomolecular Research Center, Department of Biochemistry-Microbiology, Joseph Ki-Zerbo University, Ouagadougou, Burkina Faso;Department of Biochemistry-Microbiology, University of Ddougou, Ddougou, Burkina Faso;Centre National de Recherche et de Formation sur le Paludisme, Institut National de Sant Publique, Ouagadougou, Burkina Faso;Laboratoire de biochimie et immunologie applique (LABIA), Joseph Ki-Zerbo University, Ouagadougou, Burkina Faso)

机构地区：[1]Biomolecular and Genetic Laboratory (LABIOGENE), Pietro Annigoni Biomolecular Research Center, Department of Biochemistry-Microbiology, Joseph Ki-Zerbo University, Ouagadougou, Burkina Faso [2]Department of Biochemistry-Microbiology, University of Ddougou, Ddougou, Burkina Faso [3]Centre National de Recherche et de Formation sur le Paludisme, Institut National de Sant Publique, Ouagadougou, Burkina Faso [4]Laboratoire de biochimie et immunologie applique (LABIA), Joseph Ki-Zerbo University, Ouagadougou, Burkina Faso

出　　处：《Journal of Biosciences and Medicines》2024年第11期221-232,共12页生物科学与医学（英文）

摘　　要：This study was carried out to assess the prevalence of resistance genes in strains of Escherichia coli and Salmonella spp. isolated from free-range chickens in Ouagadougou, where resistant bacteria can be transmitted to humans via faeces or contaminated meat. A total of 280 strains of Escherichia coli and 129 strains of Salmonella spp. resistant to at least one beta-lactam or carbapenem antibiotic were used in this study. PCR analyses revealed the presence of ESBL (extended spectrum beta lactamase) resistance genes in Escherichia coli isolates, with 3.21% (9/280) possessing the CTX-M (Cefotaximase) gene, 15.35% (43/280) had the SHV (Sulfhydril Variable) gene, and 11.42% (32/280) had carbapenemase resistance genes, more specifically IMP (Imipenemase metallo-beta-lactamase). As regards Salmonella spp. strains, only the presence of the SHV (Sulfhydril Variable) gene was identified in 2.32% (3/129) of isolates belonging to the ESBL family, while 26.35% (34/129) and 13.95% (18/129) of isolates respectively possessed the IMP (Imipenemase metallo-beta-lactamase) and NDM (New Delhi metallo-β-lactamase) genes, both of the carbapenemase type. The significant prevalence of resistance genes in bacterial strains isolated from chickens sold outdoors in Ouagadougou raises major public health concerns, due to the possible transmission of these resistant strains to humans through the consumption of contaminated meat, thus complicating the treatment of bacterial infections.This study was carried out to assess the prevalence of resistance genes in strains of Escherichia coli and Salmonella spp. isolated from free-range chickens in Ouagadougou, where resistant bacteria can be transmitted to humans via faeces or contaminated meat. A total of 280 strains of Escherichia coli and 129 strains of Salmonella spp. resistant to at least one beta-lactam or carbapenem antibiotic were used in this study. PCR analyses revealed the presence of ESBL (extended spectrum beta lactamase) resistance genes in Escherichia coli isolates, with 3.21% (9/280) possessing the CTX-M (Cefotaximase) gene, 15.35% (43/280) had the SHV (Sulfhydril Variable) gene, and 11.42% (32/280) had carbapenemase resistance genes, more specifically IMP (Imipenemase metallo-beta-lactamase). As regards Salmonella spp. strains, only the presence of the SHV (Sulfhydril Variable) gene was identified in 2.32% (3/129) of isolates belonging to the ESBL family, while 26.35% (34/129) and 13.95% (18/129) of isolates respectively possessed the IMP (Imipenemase metallo-beta-lactamase) and NDM (New Delhi metallo-β-lactamase) genes, both of the carbapenemase type. The significant prevalence of resistance genes in bacterial strains isolated from chickens sold outdoors in Ouagadougou raises major public health concerns, due to the possible transmission of these resistant strains to humans through the consumption of contaminated meat, thus complicating the treatment of bacterial infections.

关 键 词：BACTERIA Resistance Extended-Spectrum Beta Lactamases CARBAPENEMASES OUAGADOUGOU 

分 类 号：R37[医药卫生—病原生物学]