Effect of Hyperglycemia on Erythrocyte Carbonic Anhydrase and Lactic Acid in Type II Diabetic Subjects  

作　　者：Salihu Ismail Ibrahim Ameh Danladi Amodu Atawodi Sunday Ene-Ojo Umar Alhaji Ismaila Mohammed Fakhruddeen Salihu Ismail Ibrahim;Ameh Danladi Amodu;Atawodi Sunday Ene-Ojo;Umar Alhaji Ismaila;Mohammed Fakhruddeen(Department of Biochemistry, Federal University, Dutse, Nigeria;Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria;Department of Endocrinology, Mohammed Abdullahi Wase Specialist Hospital, Kano, Nigeria)

机构地区：[1]Department of Biochemistry, Federal University, Dutse, Nigeria [2]Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria [3]Department of Endocrinology, Mohammed Abdullahi Wase Specialist Hospital, Kano, Nigeria

出　　处：《Journal of Diabetes Mellitus》2016年第2期158-165,共8页糖尿病（英文）

摘　　要：Background: Carbonic anhydrase (CA) is a ubiquitous enzyme catalyzing the reversible hydration of CO₂ to  and H⁺. CA plays a crucial role in CO₂ transport, acid-base balance, and in linking local acidosis to O₂ unloading from hemoglobin and also facilitates lactate shuttling across the monocarboxylate transporters (MCT). The study aimed to investigate the influence of hyperglycemia on erythrocyte carbonic anhydrase activity and lactic acid in type II diabetic patients. Method: Red blood cell carbonic anhydrase activity was determined in washed lysed-hemolysate by the action of the enzyme on the substrate p-nitrophenyl acetate. The absorbance of released p-nitrophenol was recorded at 345 nm. Glycated Hemoglobin was determined by ion exchange method (Spectrum Diagnostic Kit). Blood glucose, lactate, cholesterol and triglyceride were determined using Accutrend GCT meter (Roche, Germany) with cobias^&reg test strips. Results: The present study showed that hyperglycemia significantly (p < 0.05) increased both erythrocyte carbonic anhydrase activity and blood lactate level in type II diabetic patients. Conclusion: We may conclude that hyperglycemia may be responsible for the increased activity of carbonic anhydrase activity and blood lactate concentration.Background: Carbonic anhydrase (CA) is a ubiquitous enzyme catalyzing the reversible hydration of CO₂ to  and H⁺. CA plays a crucial role in CO₂ transport, acid-base balance, and in linking local acidosis to O₂ unloading from hemoglobin and also facilitates lactate shuttling across the monocarboxylate transporters (MCT). The study aimed to investigate the influence of hyperglycemia on erythrocyte carbonic anhydrase activity and lactic acid in type II diabetic patients. Method: Red blood cell carbonic anhydrase activity was determined in washed lysed-hemolysate by the action of the enzyme on the substrate p-nitrophenyl acetate. The absorbance of released p-nitrophenol was recorded at 345 nm. Glycated Hemoglobin was determined by ion exchange method (Spectrum Diagnostic Kit). Blood glucose, lactate, cholesterol and triglyceride were determined using Accutrend GCT meter (Roche, Germany) with cobias^&reg test strips. Results: The present study showed that hyperglycemia significantly (p < 0.05) increased both erythrocyte carbonic anhydrase activity and blood lactate level in type II diabetic patients. Conclusion: We may conclude that hyperglycemia may be responsible for the increased activity of carbonic anhydrase activity and blood lactate concentration.

关 键 词：Carbonic Anhydrase Lactic Acid Glycated Hemoglobin Type II Diabetes 

分 类 号：R58[医药卫生—内分泌]