Evaluation of the Survivability of SARS-CoV-2 on Cardboard and Plastic Surfaces and the Transferability of Virus from Surface to Skin  

作　　者：Giffe T. Johnson Craig Loehle Sifang S. Zhou Cory Chiossone James Palumbo Paul Wiegand Giffe T. Johnson;Craig Loehle;Sifang S. Zhou;Cory Chiossone;James Palumbo;Paul Wiegand(National Council for Air and Stream Improvement (NCASI), Cary, USA;Microbac Laboratories, Inc., Sterling, USA)

机构地区：[1]National Council for Air and Stream Improvement (NCASI), Cary, USA [2]Microbac Laboratories, Inc., Sterling, USA

出　　处：《Occupational Diseases and Environmental Medicine》2021年第2期63-73,共11页职业病与环境医学（英文）

摘　　要：<strong>Objective: </strong>To 1) characterize the decay curve of infective SARS-CoV-2 over time on the surface of cardboard packaging and plastic mailer packaging;2) characterize the transferability over time of virus-inoculated cardboard packaging and plastic mailer packaging to skin. <strong>Methods: </strong>We inoculated samples of plastic and cardboard packaging with a titer of SARS-CoV-2 > 10⁶ TCID₅₀/ mL to evaluate the survivability and transferability to the skin (pig skin) over time. A cell culture-based infectivity assay (TCID₅₀) was used to determine viral titers. Regression analysis was used to characterize decay curves. <strong>Results:</strong> The time that SARS-CoV-2 remained transferable to skin was reduced on both packaging substrates compared to the total time of survivability, though cardboard demonstrated a substantially larger reduction. Virus inoculated plastic substrates continued to transfer the virus to the skin after 7 hours of holding time and regression analysis predicts this transferability would remain detectable up to 9.5 hours of holding time. Inoculated cardboard substrates demonstrated detectable transfer at 15 minutes of holding time, but no viable virus could be detected on the skin after 30 minutes of holding time. <strong>Conclusions:</strong> The type of material used as a packaging substrate substantially modifies the potential for SARS-CoV-2 fomite transmission. The use of materials that limit fomite transmission from packaging should be considered among strategies to reduce the transmission of SARS-CoV-2. Future research should investigate the generalizability of these findings for other viral pathogens that potentially transmit via fomite.<strong>Objective: </strong>To 1) characterize the decay curve of infective SARS-CoV-2 over time on the surface of cardboard packaging and plastic mailer packaging;2) characterize the transferability over time of virus-inoculated cardboard packaging and plastic mailer packaging to skin. <strong>Methods: </strong>We inoculated samples of plastic and cardboard packaging with a titer of SARS-CoV-2 > 10⁶ TCID₅₀/ mL to evaluate the survivability and transferability to the skin (pig skin) over time. A cell culture-based infectivity assay (TCID₅₀) was used to determine viral titers. Regression analysis was used to characterize decay curves. <strong>Results:</strong> The time that SARS-CoV-2 remained transferable to skin was reduced on both packaging substrates compared to the total time of survivability, though cardboard demonstrated a substantially larger reduction. Virus inoculated plastic substrates continued to transfer the virus to the skin after 7 hours of holding time and regression analysis predicts this transferability would remain detectable up to 9.5 hours of holding time. Inoculated cardboard substrates demonstrated detectable transfer at 15 minutes of holding time, but no viable virus could be detected on the skin after 30 minutes of holding time. <strong>Conclusions:</strong> The type of material used as a packaging substrate substantially modifies the potential for SARS-CoV-2 fomite transmission. The use of materials that limit fomite transmission from packaging should be considered among strategies to reduce the transmission of SARS-CoV-2. Future research should investigate the generalizability of these findings for other viral pathogens that potentially transmit via fomite.

关 键 词：SARS-CoV-2 Fomite CARDBOARD PLASTIC Viral Transmission to Skin 

分 类 号：TG1[金属学及工艺—金属学]