Prevalence and Antimicrobial Susceptibility Status of Gram-Negative and Gram-Positive Bacteria on Handheld Shopping Trolleys and Baskets in Supermarkets in Ndola, Zambia  

作　　者：Shivangi Patel Victor Daka Steward Mudenda Mulemba Samutela Misheck Chileshe Warren Chanda Imukusi Mutanekelwa Ephraim Chikwanda Titus Haakonde Tobela Mudenda Scott Matafwali Samson Mwale Ruth Lindizyani Mfune Shivangi Patel;Victor Daka;Steward Mudenda;Mulemba Samutela;Misheck Chileshe;Warren Chanda;Imukusi Mutanekelwa;Ephraim Chikwanda;Titus Haakonde;Tobela Mudenda;Scott Matafwali;Samson Mwale;Ruth Lindizyani Mfune(Public Health Department, School of Medicine, Copperbelt University, Ndola, Zambia;School of Health Sciences, University of Zambia, Lusaka, Zambia;Mary Begg Health Services, Ndola, Zambia;School of Medicine and Health Sciences, Mulungushi University, Livingstone, Zambia;Tropical Disease Research Centre, Ndola, Zambia;Environmental Health Section, School of Applied and Health Sciences, Evelyn Hone College of Applied Arts and Commerce, Lusaka, Zambia;Pathology and Laboratory Department, Ndola Teaching Hospital, Ndola, Zambia;Clinical Research Department, Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, London, UK)

机构地区：[1]Public Health Department, School of Medicine, Copperbelt University, Ndola, Zambia [2]School of Health Sciences, University of Zambia, Lusaka, Zambia [3]Mary Begg Health Services, Ndola, Zambia [4]School of Medicine and Health Sciences, Mulungushi University, Livingstone, Zambia [5]Tropical Disease Research Centre, Ndola, Zambia [6]Environmental Health Section, School of Applied and Health Sciences, Evelyn Hone College of Applied Arts and Commerce, Lusaka, Zambia [7]Pathology and Laboratory Department, Ndola Teaching Hospital, Ndola, Zambia [8]Clinical Research Department, Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, London, UK

出　　处：《Open Journal of Epidemiology》2023年第4期235-249,共15页流行病学期刊（英文）

摘　　要：Background: Supermarkets are a place visited by individuals with different health conditions daily where microbiological contaminants through touch onto fomites such as trolleys and baskets can be passed on to other people hence potentially spreading infectious diseases. This study aimed to investigate the presence of Gram-negative and Gram-positive bacteria on handheld shopping trolleys and baskets and their antimicrobial susceptibility status against commonly used antibiotics in Zambia. Methods: A cross-sectional study was conducted. Trolleys and basket handles were swabbed and standard microbiological methods were used to identify the bacteria and disc diffusion to determine their antimicrobial susceptibility status. Data was collected from December 2021 to April 2022. Data was analysed using IBM Statistical Package for Social Sciences (SPSS) Version 22. Results: Twenty-eight percent of the 200 total samples were found to be culture-positive and predominant isolates were Staphylococcus aureus (17.3%), Pseudomonas species (4.5%), Escherichia coli (2%), Corynebacterium species (2%), Staphylococcus species (1.5%) and Enterobacter aerogenes (0.5%). Staphylococcus aureus showed the most resistance to azithromycin (17%) followed by ciprofloxacin (2.8%), nitrofurantoin (2.8%) and chloramphenicol (2.8%). Escherichia coli showed 100% resistance to amoxicillin, cloxacillin and ampicillin, 75% resistance to ciprofloxacin and the least resistance to azithromycin (25%) while it was susceptible to nitrofurantoin. Staphylococcus species, Corynebacterium species, Enterobacter aerogenes and Pseudomonas species showed no resistance to any antibiotics. Conclusion: The study showed the presence of microorganisms with considerable antimicrobial resistance to antibiotics in Zambia on trolley and basket handles indicating the need for more initiatives to address proper hygiene in public environmental sites for better infection prevention and control.Background: Supermarkets are a place visited by individuals with different health conditions daily where microbiological contaminants through touch onto fomites such as trolleys and baskets can be passed on to other people hence potentially spreading infectious diseases. This study aimed to investigate the presence of Gram-negative and Gram-positive bacteria on handheld shopping trolleys and baskets and their antimicrobial susceptibility status against commonly used antibiotics in Zambia. Methods: A cross-sectional study was conducted. Trolleys and basket handles were swabbed and standard microbiological methods were used to identify the bacteria and disc diffusion to determine their antimicrobial susceptibility status. Data was collected from December 2021 to April 2022. Data was analysed using IBM Statistical Package for Social Sciences (SPSS) Version 22. Results: Twenty-eight percent of the 200 total samples were found to be culture-positive and predominant isolates were Staphylococcus aureus (17.3%), Pseudomonas species (4.5%), Escherichia coli (2%), Corynebacterium species (2%), Staphylococcus species (1.5%) and Enterobacter aerogenes (0.5%). Staphylococcus aureus showed the most resistance to azithromycin (17%) followed by ciprofloxacin (2.8%), nitrofurantoin (2.8%) and chloramphenicol (2.8%). Escherichia coli showed 100% resistance to amoxicillin, cloxacillin and ampicillin, 75% resistance to ciprofloxacin and the least resistance to azithromycin (25%) while it was susceptible to nitrofurantoin. Staphylococcus species, Corynebacterium species, Enterobacter aerogenes and Pseudomonas species showed no resistance to any antibiotics. Conclusion: The study showed the presence of microorganisms with considerable antimicrobial resistance to antibiotics in Zambia on trolley and basket handles indicating the need for more initiatives to address proper hygiene in public environmental sites for better infection prevention and control.

关 键 词：Antimicrobial Resistance Coliform Bacteria Staphylococcus aureus Escherichia coli SUPERMARKET Shopping Trolleys and Baskets 

分 类 号：F42[经济管理—产业经济]