Molecular Characterization of the Four Serotypes (DENV-1, DENV-2, DENV-3 and DENV-4) of Dengue Virus Circulating in Ouagadougou, Burkina Faso  

作　　者：Shoukrat Ohuwa Toyin Bello Aziz Sidi Aristide Tapsoba Abdou Azaque Zoure Yidia Jean Romaric Bassole Wend-La-Sida Kévin Yogo Prosper Bado Olawoumi Fabrice Kouta Fadilatou Tassembedo Théodora Mahoukèdè Zohoncon Florencia Wendkuuni Djigma Abdoulaye Diabate Jacques Simpore Shoukrat Ohuwa Toyin Bello;Aziz Sidi Aristide Tapsoba;Abdou Azaque Zoure;Yidia Jean Romaric Bassole;Wend-La-Sida Kévin Yogo;Prosper Bado;Olawoumi Fabrice Kouta;Fadilatou Tassembedo;Théodora Mahoukèdè Zohoncon;Florencia Wendkuuni Djigma;Abdoulaye Diabate;Jacques Simpore(African Centre of Excellence in Biotechnological Innovations for the Elimination of Vector-Borne Diseases (CEA/ITECH-MTV), Nazi BONI University, Bobo-Dioulasso, Burkina Faso;Pietro Annigoni Biomolecular Research Centre (CERBA), Ouagadougou, Burkina Faso;Laboratory of Molecular Biology and Molecular Genetics (LABIOGENE) UFR/SVT, Joseph KI-ZERBO University, Ouagadougou, Burkina Faso;Institute for Research in Health Sciences (IRSS/CNRST), Department of Biomedical and Public Health, CNRST/IRSS, Ouagadougou, Burkina Faso;Faculty of Health Sciences, Saint Thomas Aquinas University (USTA), Ouagadougou, Burkina Faso;Saint Camille Hospital in Ouagadougou (HOSCO), Ouagadougou, Burkina Faso;Institute for Research in Health Sciences (IRSS/CNRST)/Muraz Centre, Bobo-Dioulasso, Burkina Faso)

机构地区：[1]African Centre of Excellence in Biotechnological Innovations for the Elimination of Vector-Borne Diseases (CEA/ITECH-MTV), Nazi BONI University, Bobo-Dioulasso, Burkina Faso [2]Pietro Annigoni Biomolecular Research Centre (CERBA), Ouagadougou, Burkina Faso [3]Laboratory of Molecular Biology and Molecular Genetics (LABIOGENE) UFR/SVT, Joseph KI-ZERBO University, Ouagadougou, Burkina Faso [4]Institute for Research in Health Sciences (IRSS/CNRST), Department of Biomedical and Public Health, CNRST/IRSS, Ouagadougou, Burkina Faso [5]Faculty of Health Sciences, Saint Thomas Aquinas University (USTA), Ouagadougou, Burkina Faso [6]Saint Camille Hospital in Ouagadougou (HOSCO), Ouagadougou, Burkina Faso [7]Institute for Research in Health Sciences (IRSS/CNRST)/Muraz Centre, Bobo-Dioulasso, Burkina Faso

出　　处：《Open Journal of Epidemiology》2024年第4期565-578,共14页流行病学期刊（英文）

摘　　要：Background and Objectives: Dengue is an arbovirosis caused by the dengue virus with 04 serotypes. The aim of the study was to characterise the four serotypes (DENV-1, DENV-2, DENV-3 and DENV-4) of the dengue virus circulating in Ouagadougou, Burkina Faso. Methods: This was a descriptive analytical study that included 2833 patients and was carried out from January 2021 to December 2022. Rapid diagnosis of dengue was performed using the “Dengue Duo (AgNS1/IgM/IgG)” kit (SD Bioline, Korea). Viral RNA was extracted using the QIAGEN RNA RNeasy Plus Mini Kit (Quiagen, Germany) and virus serotypes were identified using the DENGUE Real-TM Genotype PCR kit (Sacace biotechnologies, Italy). Platelet counts were also performed using the XN-1500 Sysmex. Results: The prevalence of acute infections (NS1Ag positive) by TDR was 5.7% (162/2833), with the peak of dengue virus infection occurring between October and November. On the other hand, the AgNS1+ samples tested by RT-PCR were 53.7% positive for dengue virus;this shows the extent of probable cross-reactions with rapid diagnostic tests and false positives. Serotype 1 accounted for 52.6%, 28.4% had serotype 3, 16.8% had serotype 2 and 2.1% had serotype 4. We found cases of co-infection with DENV-1 and DENV-2 in two patients, co-infection with DENV-1 and DENV-3 in three patients, co-infection with DENV-1 and DENV-4 in one patient, co-infection with DENV-3 and DENV-4 in one patient and co-infection with three serotypes, DENV-1, DENV-2 and DENV-3 in one patient. Conclusion: The study showed that all four serotypes of the dengue virus were circulating in Ouagadougou. Serotype 1 was predominant.Background and Objectives: Dengue is an arbovirosis caused by the dengue virus with 04 serotypes. The aim of the study was to characterise the four serotypes (DENV-1, DENV-2, DENV-3 and DENV-4) of the dengue virus circulating in Ouagadougou, Burkina Faso. Methods: This was a descriptive analytical study that included 2833 patients and was carried out from January 2021 to December 2022. Rapid diagnosis of dengue was performed using the “Dengue Duo (AgNS1/IgM/IgG)” kit (SD Bioline, Korea). Viral RNA was extracted using the QIAGEN RNA RNeasy Plus Mini Kit (Quiagen, Germany) and virus serotypes were identified using the DENGUE Real-TM Genotype PCR kit (Sacace biotechnologies, Italy). Platelet counts were also performed using the XN-1500 Sysmex. Results: The prevalence of acute infections (NS1Ag positive) by TDR was 5.7% (162/2833), with the peak of dengue virus infection occurring between October and November. On the other hand, the AgNS1+ samples tested by RT-PCR were 53.7% positive for dengue virus;this shows the extent of probable cross-reactions with rapid diagnostic tests and false positives. Serotype 1 accounted for 52.6%, 28.4% had serotype 3, 16.8% had serotype 2 and 2.1% had serotype 4. We found cases of co-infection with DENV-1 and DENV-2 in two patients, co-infection with DENV-1 and DENV-3 in three patients, co-infection with DENV-1 and DENV-4 in one patient, co-infection with DENV-3 and DENV-4 in one patient and co-infection with three serotypes, DENV-1, DENV-2 and DENV-3 in one patient. Conclusion: The study showed that all four serotypes of the dengue virus were circulating in Ouagadougou. Serotype 1 was predominant.

关 键 词：Dengue Virus SEROTYPES RT-PCR Burkina Faso 

分 类 号：R51[医药卫生—内科学]