Phytochemical Analysis and Contractile Effects of Aqueous and Hydroethanolic Extracts of Anastatica hierochuntica L. (Brassicaceae) on the Isolated Uterus of Mice  

作　　者：Belem-Kabré Wendkouni Leila Marie Esther Nitiéma Mathieu Odjo Blondine Saïdath Maman Noura Oumarou Yaro Boubacar Kaboré Boukaré Somda Gaëtan Donzéo Traoré Tata Kadiatou Koala Moumouni Ilboudo Sylvain Ouédraogo Moussa Kabré Elie Ouédraogo Noufou Belem-Kabré Wendkouni Leila Marie Esther;Nitiéma Mathieu;Odjo Blondine Saïdath;Maman Noura Oumarou;Yaro Boubacar;Kaboré Boukaré;Somda Gaëtan Donzéo;Traoré Tata Kadiatou;Koala Moumouni;Ilboudo Sylvain;Ouédraogo Moussa;Kabré Elie;Ouédraogo Noufou(Laboratoire de Recherche-Développement de Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS/CNRST), Ouagadougou, Burkina Faso;Laboratoire du Développement du Médicament (LADME)/Centre de Formation, de Recherche et d’Expertises en Sciences du Médicament (CEA-CFOREM), Université Joseph KI-ZERBO, Ouagadougou, Burkina Faso;Laboratoire de Chimie Organique et de Physique Appliquée (LCOPA), Université Joseph KI-ZERBO, Ouagadougou, Burkina Faso;Agence Nationale pour la Sécurité Sanitaire de l’Environnement, de l’Alimentation, du Travail et des Produits de Santé (ANSSEAT), Ouagadougou, Burkina Faso)

机构地区：[1]Laboratoire de Recherche-Développement de Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS/CNRST), Ouagadougou, Burkina Faso [2]Laboratoire du Développement du Médicament (LADME)/Centre de Formation, de Recherche et d’Expertises en Sciences du Médicament (CEA-CFOREM), Université Joseph KI-ZERBO, Ouagadougou, Burkina Faso [3]Laboratoire de Chimie Organique et de Physique Appliquée (LCOPA), Université Joseph KI-ZERBO, Ouagadougou, Burkina Faso [4]Agence Nationale pour la Sécurité Sanitaire de l’Environnement, de l’Alimentation, du Travail et des Produits de Santé (ANSSEAT), Ouagadougou, Burkina Faso

出　　处：《Pharmacology & Pharmacy》2023年第8期252-270,共19页药理与制药（英文）

摘　　要：Background: Anastatica hierochuntica L. (Brassicaceae) has been claimed to treat various conditions, including complicated childbirth. The principal objective of this research was to provide scientific evidence on the contractile effect of the aqueous and hydroethanolic extracts of the whole plant from A. hierochuntica during labor. Method: Qualitative and quantitative methods helped identify and quantify the phytoconstituents in both extracts. The antioxidant activity was studied using four standard methods (ABTS, DPPH, FRAP, and LPO). Results: The inhibitory power of the extracts on pro-inflammatory enzymes such as 15-lipoxygenase and phospholipase A₂ was determined. The contractile effects of the extracts were evaluated using isolated pregnant mice uteri. Steroids, triterpenoids, flavonoids, tannins, coumarins, and reduced compounds were identified in both extracts. The hydroethanolic extract exhibited higher content of hydrolyzable tannins (15.07 ± 0.24 mg tannic acid equivalent/g) than the aqueous decoction (11.8 ± 0.69 mg TAE/g). The extracts demonstrated the ability to scavenge DPPH and ABTS radicals, reduce ferric ions, and inhibit especially lipid peroxidation. No significant difference was noted in the phospholipase inhibition between the extracts and betamethasone. The hydroethanolic extract displayed the most significant anti-lipoxygenase activity with an IC₅₀ value of 55.82 ± 1.15 μg/mL. The lethal dose (LD₅₀) of extracts was estimated to be superior to 2000 mg/kg body weight (bw). The ability of the samples to produce one cm amplitude of contraction at 50% effective concentrations (EC₅₀) was 5.33 × 10⁻⁸ ± 0.32 mg/mL for oxytocin, 1.51 ± 0.08 mg/mL for aqueous extract and 3.57 ± 0.61 mg/mL for hydroethanolic extract. Conclusion: These findings could provide evidence for using Anastatica hierochuntica to facilitate childbirth.Background: Anastatica hierochuntica L. (Brassicaceae) has been claimed to treat various conditions, including complicated childbirth. The principal objective of this research was to provide scientific evidence on the contractile effect of the aqueous and hydroethanolic extracts of the whole plant from A. hierochuntica during labor. Method: Qualitative and quantitative methods helped identify and quantify the phytoconstituents in both extracts. The antioxidant activity was studied using four standard methods (ABTS, DPPH, FRAP, and LPO). Results: The inhibitory power of the extracts on pro-inflammatory enzymes such as 15-lipoxygenase and phospholipase A₂ was determined. The contractile effects of the extracts were evaluated using isolated pregnant mice uteri. Steroids, triterpenoids, flavonoids, tannins, coumarins, and reduced compounds were identified in both extracts. The hydroethanolic extract exhibited higher content of hydrolyzable tannins (15.07 ± 0.24 mg tannic acid equivalent/g) than the aqueous decoction (11.8 ± 0.69 mg TAE/g). The extracts demonstrated the ability to scavenge DPPH and ABTS radicals, reduce ferric ions, and inhibit especially lipid peroxidation. No significant difference was noted in the phospholipase inhibition between the extracts and betamethasone. The hydroethanolic extract displayed the most significant anti-lipoxygenase activity with an IC₅₀ value of 55.82 ± 1.15 μg/mL. The lethal dose (LD₅₀) of extracts was estimated to be superior to 2000 mg/kg body weight (bw). The ability of the samples to produce one cm amplitude of contraction at 50% effective concentrations (EC₅₀) was 5.33 × 10⁻⁸ ± 0.32 mg/mL for oxytocin, 1.51 ± 0.08 mg/mL for aqueous extract and 3.57 ± 0.61 mg/mL for hydroethanolic extract. Conclusion: These findings could provide evidence for using Anastatica hierochuntica to facilitate childbirth.

关 键 词：Anastatica hierochuntica PHYTOCONSTITUENTS ANTIOXIDANT ANTI-INFLAMMATORY Uterine Contractility 

分 类 号：R28[医药卫生—中药学]