Acute Toxicity and Anthelmintic Effects of Gomphrena serrata L. (Amaranthaceae) Extracts on Adult Worms and Eggs of Haemonchus contortus  

作　　者：Hippolyte Ouedraogo Mohamed Bonewendé Belemlilga Aristide Traore Gilchrist Abdoul Laurent Boly Félicité Moyenga Gueswindé Geoffroy Ouedraogo Sylvain Ilboudo Lazare Belemnaba Sylvin Ouedraogo Hippolyte Ouedraogo;Mohamed Bonewendé Belemlilga;Aristide Traore;Gilchrist Abdoul Laurent Boly;Félicité Moyenga;Gueswindé Geoffroy Ouedraogo;Sylvain Ilboudo;Lazare Belemnaba;Sylvin Ouedraogo(Laboratoire de Recherche-Dé,veloppement de Phytomé,dicaments et Mé,dicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé, (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST), Ouagadougou, Burkina Faso;Laboratoire de Dé,veloppement du Mé,dicament (LADME), Centre de Formation, deRecherche et d’,Expertises en Sciences du Mé,dicament (CEA-CFOREM), É,cole Doctorale Sciences de la Santé, (ED2S), Université, Joseph KI-ZERBO, Ouagadougou, Burkina Faso;International Research Laboratory-Environnement, Santé, Socié,té,s (IRL 3189, ESS) CNRS/CNRST/UCAD/UGB/USTTB, Ouagadougou, Burkina Faso)

机构地区：[1]Laboratoire de Recherche-Dé,veloppement de Phytomé,dicaments et Mé,dicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé, (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST), Ouagadougou, Burkina Faso [2]Laboratoire de Dé,veloppement du Mé,dicament (LADME), Centre de Formation, deRecherche et d’,Expertises en Sciences du Mé,dicament (CEA-CFOREM), É,cole Doctorale Sciences de la Santé, (ED2S), Université, Joseph KI-ZERBO, Ouagadougou, Burkina Faso [3]International Research Laboratory-Environnement, Santé, Socié,té,s (IRL 3189, ESS) CNRS/CNRST/UCAD/UGB/USTTB, Ouagadougou, Burkina Faso

出　　处：《Pharmacology & Pharmacy》2024年第5期207-221,共15页药理与制药（英文）

摘　　要：Gomphrena serrata L. (Amaranthaceae) is a medicinal plant widely used traditionally in rural areas to treat many diseases, including gastrointestinal helminthiasis of small ruminants. This work aims to estimate the plant extracts phytochemical potential and study the activities of the aqueous decoction (GS_AD), aqueous macerate (GS_AM), and hydroalcoholic macerate (GS_HEM) of Gomphrena serrata extracts on two developmental stages (eggs and adult worms) of Haemonchus contortus. Phytochemical screening was carried out. The acute toxicity test was performed according to the Organisation for Economic Co-operation and Development (OECD) test guideline 423. The Haemonchus contortus eggs hatching inhibition test was performed left to incubate for 48 hours in the presence of the different plant extracts at increasing concentrations of 0.125;0.25;0.5;0.625;1;2;3 mg/mL. For the motility inhibition test on adult worms, the worms were contacted with the extracts at concentrations of 0.75;1;1.25;1.5;2;3;4 mg/mL and left to incubate for 24 hours. Albendazole and levamisole were used as standard. Phytochemical screening revealed the presence of tannins, saponins, reducing compounds, coumarins and derivatives, anthocyanins, steroids, triterpenes, and flavonoids. An acute toxicity study showed that the oral LD₅₀ value of G. serrata was estimated to be 5000 mg/kg. All extracts showed an effect on both stages of parasite development. The hatching inhibition rate was 100% from 3 mg/mL for all three extracts. The rate of inhibition of motility was 100% from 4 mg/mL for each extract. HEM is more efficient on eggs and Haemonchus contortus adult worms than other extracts with IC₅₀ of 0.457 mg/mL and LC₅₀of 1.329 mg/mL, respectively. These results suggest that Gomphrena serrata, a plant used by farmers, has anthelmintic properties.Gomphrena serrata L. (Amaranthaceae) is a medicinal plant widely used traditionally in rural areas to treat many diseases, including gastrointestinal helminthiasis of small ruminants. This work aims to estimate the plant extracts phytochemical potential and study the activities of the aqueous decoction (GS_AD), aqueous macerate (GS_AM), and hydroalcoholic macerate (GS_HEM) of Gomphrena serrata extracts on two developmental stages (eggs and adult worms) of Haemonchus contortus. Phytochemical screening was carried out. The acute toxicity test was performed according to the Organisation for Economic Co-operation and Development (OECD) test guideline 423. The Haemonchus contortus eggs hatching inhibition test was performed left to incubate for 48 hours in the presence of the different plant extracts at increasing concentrations of 0.125;0.25;0.5;0.625;1;2;3 mg/mL. For the motility inhibition test on adult worms, the worms were contacted with the extracts at concentrations of 0.75;1;1.25;1.5;2;3;4 mg/mL and left to incubate for 24 hours. Albendazole and levamisole were used as standard. Phytochemical screening revealed the presence of tannins, saponins, reducing compounds, coumarins and derivatives, anthocyanins, steroids, triterpenes, and flavonoids. An acute toxicity study showed that the oral LD₅₀ value of G. serrata was estimated to be 5000 mg/kg. All extracts showed an effect on both stages of parasite development. The hatching inhibition rate was 100% from 3 mg/mL for all three extracts. The rate of inhibition of motility was 100% from 4 mg/mL for each extract. HEM is more efficient on eggs and Haemonchus contortus adult worms than other extracts with IC₅₀ of 0.457 mg/mL and LC₅₀of 1.329 mg/mL, respectively. These results suggest that Gomphrena serrata, a plant used by farmers, has anthelmintic properties.

关 键 词：Gomphrena serrata Haemonchus contortus ANTHELMINTIC In Vitro 

分 类 号：R28[医药卫生—中药学]