Curative Effect of Spondias mombin L. against Carbon Tetrachloride-Induced Liver Damage in Rat  

作　　者：Assi Narcisse Roméo Boni Agnon Prisca Djoupo Konan Kouassi Jean-David N’Guessan Assi Narcisse Roméo Boni;Agnon Prisca Djoupo;Konan Kouassi;Jean-David N’Guessan(Training and Research Unit of Sciences and Technologies, Alassane Ouattara University, Bouak, Cte dIvoire;Laboratory of Biochemistry, Faculty of Medicine, Alassane Ouattara University, Bouak, Cte dIvoire;Laboratory of Biology and Health, Flix Houphout-Boigny University, Abidjan, Cte dIvoire)

机构地区：[1]Training and Research Unit of Sciences and Technologies, Alassane Ouattara University, Bouak, Cte dIvoire [2]Laboratory of Biochemistry, Faculty of Medicine, Alassane Ouattara University, Bouak, Cte dIvoire [3]Laboratory of Biology and Health, Flix Houphout-Boigny University, Abidjan, Cte dIvoire

出　　处：《Pharmacology & Pharmacy》2024年第12期478-489,共12页药理与制药(英文)

摘　　要：Background: Oxidative stress is implicated in liver disease pathogenesis. Liver injuries are traditionally treated with stem bark from Spondias mombin. In our previous study, this plant demonstrated promising in vitro antioxidant activities. Objective: The purpose of this study was to evaluate the antioxidant and hepatocurative activity of methanolic extract of S. mombin (MESPM) through in vivo studies. Methods: Thirty rats were divided into five groups containing six rats each. Group I was the normal control, group II was the negative control and the other groups were experimental groups. Rats in groups II - V received, firstly, 2 mL/kg/day of carbon tetrachloride (CCl4) intraperitoneally, for three days to induce oxidative stress and hepatotoxicity. On the following day, groups IV and V were administered MESPM orally for eleven consecutive days, respectively, at 200 and 400 mg/kg/day. Silymarin (50 mg/kg) was used as a standard (group II: positive control). Serum biochemical parameters and in vivo antioxidant activity were measured using standard procedures. Gross and histopathological studies were also performed. Results: CCl4 induced oxidative stress was manifested by an increase in TBARS level as well as a decrease in FRAP level and DPPH radicals scavenging percentage compared to normal control (P 4 hepatotoxicity caused significant increases in ALT, AST, ALP, bilirubin levels, with a decline in total proteins (P 4 confirmed that MESPM was effective. Conclusion: The results confirm MESPM’s traditional use as a hepatocurative agent, probably due to its antioxidant properties.Background: Oxidative stress is implicated in liver disease pathogenesis. Liver injuries are traditionally treated with stem bark from Spondias mombin. In our previous study, this plant demonstrated promising in vitro antioxidant activities. Objective: The purpose of this study was to evaluate the antioxidant and hepatocurative activity of methanolic extract of S. mombin (MESPM) through in vivo studies. Methods: Thirty rats were divided into five groups containing six rats each. Group I was the normal control, group II was the negative control and the other groups were experimental groups. Rats in groups II - V received, firstly, 2 mL/kg/day of carbon tetrachloride (CCl4) intraperitoneally, for three days to induce oxidative stress and hepatotoxicity. On the following day, groups IV and V were administered MESPM orally for eleven consecutive days, respectively, at 200 and 400 mg/kg/day. Silymarin (50 mg/kg) was used as a standard (group II: positive control). Serum biochemical parameters and in vivo antioxidant activity were measured using standard procedures. Gross and histopathological studies were also performed. Results: CCl4 induced oxidative stress was manifested by an increase in TBARS level as well as a decrease in FRAP level and DPPH radicals scavenging percentage compared to normal control (P 4 hepatotoxicity caused significant increases in ALT, AST, ALP, bilirubin levels, with a decline in total proteins (P 4 confirmed that MESPM was effective. Conclusion: The results confirm MESPM’s traditional use as a hepatocurative agent, probably due to its antioxidant properties.

关 键 词：Oxidative Stress Hepatocurative ANTIOXIDANT Spondias mombin 

分 类 号：R28[医药卫生—中药学]