Genetic Diversity of Indigenous Chicken (Gallus Gallus domesticus) from Ecozones of Egypt and Kingdom of Saudi Arabia  

作　　者：Ayman Sabry Alaa Ahmed Mohamed Mohamed Hassen Ayman Sabry;Alaa Ahmed Mohamed;Mohamed Hassen(Biology Department, Faculty of Science, Taif University, Taif, KSA;Cell Biology Department, National Research Center, Dokki, Giza, Egypt;Department of Animal Reproduction and AI, National Research Center, Dokki, Giza, Egypt;Department of Genetics, Faculty of Agriculture, Minufiya University, Al Minufya, Egypt)

机构地区：[1]Biology Department, Faculty of Science, Taif University, Taif, KSA [2]Cell Biology Department, National Research Center, Dokki, Giza, Egypt [3]Department of Animal Reproduction and AI, National Research Center, Dokki, Giza, Egypt [4]Department of Genetics, Faculty of Agriculture, Minufiya University, Al Minufya, Egypt

出　　处：《Open Journal of Applied Sciences》2021年第6期775-787,共13页应用科学（英文）

摘　　要：Genetic diversity of two chicken ecotypes from Ismailia-Egypt (ISM) and Taif-Saudi Arabia (TA) was evaluated using 39 microsatellites. DNA was extracted from blood of 25 chickens/ecotype. The number of alleles was 157 and 138, the number of alleles/locus averaged 4.2±2.2 and 3.6±1.6, and the highest number of private alleles was 9 and 5 for ISM and TA, respectively. Percentage of shared alleles between the two ecotypes was 45%. This panel of markers is reasonably informative as the mean polymorphic information content for ISM and TA was 0.47±0.21, and 0.41±0.2. Similar average of observed heterozygosity was attained for both ecotypes. Conversely, averages of expected heterozygosity differed between two ecotypes, 0.52±0.23 vs. 0.45±0.21 for ISM and TA. 8 and 12 loci have significantly deviated from HWE of ISM and TA. Estimate of genetic distance was 0.2 and F_ST index was 0.29. Results showed only 6% of genetic diversity is shared between these two ecotypes.Genetic diversity of two chicken ecotypes from Ismailia-Egypt (ISM) and Taif-Saudi Arabia (TA) was evaluated using 39 microsatellites. DNA was extracted from blood of 25 chickens/ecotype. The number of alleles was 157 and 138, the number of alleles/locus averaged 4.2±2.2 and 3.6±1.6, and the highest number of private alleles was 9 and 5 for ISM and TA, respectively. Percentage of shared alleles between the two ecotypes was 45%. This panel of markers is reasonably informative as the mean polymorphic information content for ISM and TA was 0.47±0.21, and 0.41±0.2. Similar average of observed heterozygosity was attained for both ecotypes. Conversely, averages of expected heterozygosity differed between two ecotypes, 0.52±0.23 vs. 0.45±0.21 for ISM and TA. 8 and 12 loci have significantly deviated from HWE of ISM and TA. Estimate of genetic distance was 0.2 and F_ST index was 0.29. Results showed only 6% of genetic diversity is shared between these two ecotypes.

关 键 词：ECOTYPES Genetic Diversity MICROSATELLITE Polymorphic Information Content Hardy Weinberg Expectation 

分 类 号：S51[农业科学—作物学]