Evaluation of Subacute Toxicity of Hydroethanolic Extracts Combinations from Gnetum africanum (Welv.) and Gnetum buchholzianum (Engl.) (Genetaceae) Leaves: Two Botanical with Antiproliferative and Antioxidant Potential  

作　　者：Bertin Sone Enone Jean-Pierre Ngene Angèle Foyet Fondjo Charles Christian Ngoule Loé Gisèle Etame Loick Pradel Kojom Foko Christophe Manz Koule François Siewe Ronald Bidingha Goufani Josiane Etang Albert Mouelle Sone Bertin Sone Enone;Jean-Pierre Ngene;Angèle Foyet Fondjo;Charles Christian Ngoule;Loé Gisèle Etame;Loick Pradel Kojom Foko;Christophe Manz Koule;François Siewe;Ronald Bidingha Goufani;Josiane Etang;Albert Mouelle Sone(Laboratory of Microbiology, Immunology-Hematology and Morphological Sciences, Doctoral Training Unit of Health Sciences, Faculty of Medicine and Pharmaceutical Sciences, Douala University, Douala, Cameroon;Department of Pharmaceutical Sciences, Faculty of Medicine and Pharmaceutical Sciences, Douala University, Douala, Cameroon;Department of Medical Health Sciences, Golf of Guinea University Institute (IUG), Douala, Cameroon;Department of Animal Biology and Physiology, Faculty of Science, University of Douala, Douala, Cameroon;Department of Biochemistry and Nutrition, Faculty of Science, University of Douala, Douala, Cameroon;Department of Technology and Biomedical Sciences, Golf of Guinea University Institut (IUG), Douala, Cameroon;Laboratory of Animal Physiology, Department of Animal Biology, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon)

机构地区：[1]Laboratory of Microbiology, Immunology-Hematology and Morphological Sciences, Doctoral Training Unit of Health Sciences, Faculty of Medicine and Pharmaceutical Sciences, Douala University, Douala, Cameroon [2]Department of Pharmaceutical Sciences, Faculty of Medicine and Pharmaceutical Sciences, Douala University, Douala, Cameroon [3]Department of Medical Health Sciences, Golf of Guinea University Institute (IUG), Douala, Cameroon [4]Department of Animal Biology and Physiology, Faculty of Science, University of Douala, Douala, Cameroon [5]Department of Biochemistry and Nutrition, Faculty of Science, University of Douala, Douala, Cameroon [6]Department of Technology and Biomedical Sciences, Golf of Guinea University Institut (IUG), Douala, Cameroon [7]Laboratory of Animal Physiology, Department of Animal Biology, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon

出　　处：《Open Journal of Applied Sciences》2022年第6期944-963,共20页应用科学（英文）

摘　　要：This study was conducted to assess the toxic effect of hydroethanolic extract combinations of Gnetum africanum Welv. leaves and Gnetum buchholzianum Engl. (Gnetaceae) in experimental rats to test the validity of the treatment of liver disorders related to oxidative stress. The Combinations (m/m) 50-50 for E2 and 75-25 for E3 of ethanol-water extracts from plant leaves at the respective doses of 100, 200, and 400 mg/kg of body weight were used for 32-day toxicity. They were obtained after harvesting leaves, sorting, drying in the air cover for three weeks, and grinding. The resulting powder was doubly macerated with 70% ethanol for 48 hours and filtered. The filtrate was concentrated with the Heidolph-brand rotary rotavapor and each extract obtained was preserved. The administrations were carried out by gavage to wistar, male and non-pregnant female albino rats. In the end, the animals were sacrificed. The serum and organ homogenates were obtained for biochemical, tissue, and histopathological analyses respectively. The analyses revealed insignificant variations at the 5% probability threshold of the weight growth of experimental animals. These variations were found to be statistically significant at the same probability for biochemical and tissue parameters based on the dose of plant extracts and compared to control animals. Histopathological analysis of liver tissue showed leukodate infiltration that indicates extract-induced inflammation of the hepatocytes at the 400 mg/kg dose of body weight in females. However, this infiltration of the cells would have improved the regeneration of hepatocytes justified by the normal rate of transaminases. These results showed that combinations of hydroethanolic extracts of G. africanum and G. buchholzianumare non-toxicand may be potential candidates in the Cameroonian flora medicinal plant database shown in the monitoring of oxidative stress-related diseases.This study was conducted to assess the toxic effect of hydroethanolic extract combinations of Gnetum africanum Welv. leaves and Gnetum buchholzianum Engl. (Gnetaceae) in experimental rats to test the validity of the treatment of liver disorders related to oxidative stress. The Combinations (m/m) 50-50 for E2 and 75-25 for E3 of ethanol-water extracts from plant leaves at the respective doses of 100, 200, and 400 mg/kg of body weight were used for 32-day toxicity. They were obtained after harvesting leaves, sorting, drying in the air cover for three weeks, and grinding. The resulting powder was doubly macerated with 70% ethanol for 48 hours and filtered. The filtrate was concentrated with the Heidolph-brand rotary rotavapor and each extract obtained was preserved. The administrations were carried out by gavage to wistar, male and non-pregnant female albino rats. In the end, the animals were sacrificed. The serum and organ homogenates were obtained for biochemical, tissue, and histopathological analyses respectively. The analyses revealed insignificant variations at the 5% probability threshold of the weight growth of experimental animals. These variations were found to be statistically significant at the same probability for biochemical and tissue parameters based on the dose of plant extracts and compared to control animals. Histopathological analysis of liver tissue showed leukodate infiltration that indicates extract-induced inflammation of the hepatocytes at the 400 mg/kg dose of body weight in females. However, this infiltration of the cells would have improved the regeneration of hepatocytes justified by the normal rate of transaminases. These results showed that combinations of hydroethanolic extracts of G. africanum and G. buchholzianumare non-toxicand may be potential candidates in the Cameroonian flora medicinal plant database shown in the monitoring of oxidative stress-related diseases.

关 键 词：Gnetum africanum Gnetum buchholzianum Subacute Toxicity Oxidative Stress Albino Rats 

分 类 号：O63[理学—高分子化学]