Characterization and Quantification of Phenolic Compounds of Hydroethanolic Extracts and Fractions of Leaves Gnetum africanum (Welv.) and Gnetum buchholzianum (Engl.) (Gnetaceae)  

作　　者：Bertin Sone Enone Gisèle Marie-Marguerite Etame-Loe Charles Christian Ngoule Jean-Pierre Ngene Juliette Koube Lahngong Methodius Shinyuy Simone Véronique Fannang Moïse Henri Julien Nko’o Jean Baptiste Hzounda Fokou Hans Denis Bamal Celestine Magne Valdi Steve Djova Wilfred Ndogmo Tekapi Sandrine Sikadeu Josiane Etang Albert Mouelle Sone Bertin Sone Enone;Gisèle Marie-Marguerite Etame-Loe;Charles Christian Ngoule;Jean-Pierre Ngene;Juliette Koube;Lahngong Methodius Shinyuy;Simone Véronique Fannang;Moïse Henri Julien Nko’o;Jean Baptiste Hzounda Fokou;Hans Denis Bamal;Celestine Magne;Valdi Steve Djova;Wilfred Ndogmo Tekapi;Sandrine Sikadeu;Josiane Etang;Albert Mouelle Sone(Laboratory of Microbiology, Immunology-Hematology and Morphological Sciences Doctoral Training Unit in Health Sciences, Faculty of Medicine and Pharmaceutical Sciences, University of Douala, Douala, Cameroon;Department of Pharmaceutical Sciences, Faculty of Medicine and Pharmaceutical Sciences, University of Douala, Douala, Cameroon;Department of Microbiology, University of Yaounde I, Yaounde, Cameroon;Chemistry Laboratory, Faculty of Sciences, University of Douala, Douala, Cameroon;Department of Biology and plant Organisms, Faculty of Science, Douala, Cameroon)

机构地区：[1]Laboratory of Microbiology, Immunology-Hematology and Morphological Sciences Doctoral Training Unit in Health Sciences, Faculty of Medicine and Pharmaceutical Sciences, University of Douala, Douala, Cameroon [2]Department of Pharmaceutical Sciences, Faculty of Medicine and Pharmaceutical Sciences, University of Douala, Douala, Cameroon [3]Department of Microbiology, University of Yaounde I, Yaounde, Cameroon [4]Chemistry Laboratory, Faculty of Sciences, University of Douala, Douala, Cameroon [5]Department of Biology and plant Organisms, Faculty of Science, Douala, Cameroon

出　　处：《Open Journal of Applied Sciences》2022年第7期1304-1318,共15页应用科学（英文）

摘　　要：Research for bioactive molecules and not resistant to infectious agents remains topical for science. The total polyphenols (TPP) and total flavonoids (TFv) of the hydroethanolic extracts and fractions obtained were quantified according to the spectrophotometric method described in the literature using the Folin-Ciocalteu reagent and the colorless solutions of sodium nitrite 2.5% and aluminum chloride 10% respectively. The characterization of chemical compounds was made by coupling liquid chromatography (LC) and mass spectroscopy (MS). Thus, 15 chemical compounds were characterized, seven from Gnetum africanum (EBa) and eight from Gnetum buchholzianum (EBb). The majority of which were stilbens, such as Gnetupendin D, Dimethoxygnetulin, Methoxyparvifolol D, Isorhaponcitin from Eba and Gnetuhaidin P, Gnetupendin D, Gnetuhainin C, phenols (Alcohol Homovanillyl, Alcohol Erythro guaiacylglycerol-β-O-4'-coniferyl, and Alcohol Homovanillyl from EBb. The minority were flavonoids such as Dimethoxydihydropyraneriodictyol, Dimethoxyeriodictyol (EBa) and Dimethoxydihydropyraneriodictyol, Trimethoxyluteolin (EBb). The levels of total polyphenolics and total flavonoids content were respectively 14.520 ± 0.557 mg EAA/g Extract and 29.870 ± 2.485 mg EQ/g Extract for EBa. The levels of total polyphenolics and total flavonoids content for EBb were respectively 4.560 ± 0.010 mg EAA/g Extract and 2.233 ± 0.251 mg EQ/g Extract. The high levels of flavonoids content respectively 220.200 ± 35.500 and 335.300 ± 57.810 mg EQ/g Extract were shown in Dichlorometan fraction from EBa and EBb. These bioactive compounds identified and total phenolics compounds quantified can be exploited in pharmacological and pharmacokinetical activities to fight against multi-resistant infectious agents and as well as oxidative stress and antiinflammatory diseases.Research for bioactive molecules and not resistant to infectious agents remains topical for science. The total polyphenols (TPP) and total flavonoids (TFv) of the hydroethanolic extracts and fractions obtained were quantified according to the spectrophotometric method described in the literature using the Folin-Ciocalteu reagent and the colorless solutions of sodium nitrite 2.5% and aluminum chloride 10% respectively. The characterization of chemical compounds was made by coupling liquid chromatography (LC) and mass spectroscopy (MS). Thus, 15 chemical compounds were characterized, seven from Gnetum africanum (EBa) and eight from Gnetum buchholzianum (EBb). The majority of which were stilbens, such as Gnetupendin D, Dimethoxygnetulin, Methoxyparvifolol D, Isorhaponcitin from Eba and Gnetuhaidin P, Gnetupendin D, Gnetuhainin C, phenols (Alcohol Homovanillyl, Alcohol Erythro guaiacylglycerol-β-O-4'-coniferyl, and Alcohol Homovanillyl from EBb. The minority were flavonoids such as Dimethoxydihydropyraneriodictyol, Dimethoxyeriodictyol (EBa) and Dimethoxydihydropyraneriodictyol, Trimethoxyluteolin (EBb). The levels of total polyphenolics and total flavonoids content were respectively 14.520 ± 0.557 mg EAA/g Extract and 29.870 ± 2.485 mg EQ/g Extract for EBa. The levels of total polyphenolics and total flavonoids content for EBb were respectively 4.560 ± 0.010 mg EAA/g Extract and 2.233 ± 0.251 mg EQ/g Extract. The high levels of flavonoids content respectively 220.200 ± 35.500 and 335.300 ± 57.810 mg EQ/g Extract were shown in Dichlorometan fraction from EBa and EBb. These bioactive compounds identified and total phenolics compounds quantified can be exploited in pharmacological and pharmacokinetical activities to fight against multi-resistant infectious agents and as well as oxidative stress and antiinflammatory diseases.

关 键 词：Gnetum africanum Gnetum buchholzianum Phenolic Compounds Liquid Chromatography Mass Spectroscopy 

分 类 号：O63[理学—高分子化学]