Optimization and Validation of a Simple Spectrophotometric Based DPPH Method for Analysis of Antioxidant Activity in Aerated, Semi-Aerated and Non-Aerated Tea Products  

作　　者：Simon Mwangi Kingori Sylvester Cheruiyot Aron Cheruiyot Kirui Rugema Grace Uwamahoro Agnes Wangui Mwangi Simon Mwangi Kingori;Sylvester Cheruiyot;Aron Cheruiyot Kirui;Rugema Grace Uwamahoro;Agnes Wangui Mwangi(Kenya Agricultural and Livestock Research Organization-Tea Research Institute, Kericho, Kenya;Department of Chemistry, Egerton University, Egerton, Kenya;Department of Food Science, Dedan Kimathi University of Technology, Nyeri, Kenya;Faculty of Food Science, Hungarian University of Agriculture and Life Sciences, Budapest, Hungary)

机构地区：[1]Kenya Agricultural and Livestock Research Organization-Tea Research Institute, Kericho, Kenya [2]Department of Chemistry, Egerton University, Egerton, Kenya [3]Department of Food Science, Dedan Kimathi University of Technology, Nyeri, Kenya [4]Faculty of Food Science, Hungarian University of Agriculture and Life Sciences, Budapest, Hungary

出　　处：《Open Journal of Applied Sciences》2024年第8期2207-2222,共16页应用科学（英文）

摘　　要：In this study, the simple DPPH UV-spectrophotometric analytical procedures were optimized and validated for the estimation of antioxidant properties in aerated, semi-aerated and non-aerated tea products as per the AOAC guidelines. The method was found to be simple, rapid, accurate and economical in estimating antioxidant properties in the different tea products. A (0.0100 ± 0.0001) g well ground tea sample was extracted directly in 80% v/v methanol in distilled water. The precision of the method had an RSD% of 1.695 with a linear relation equation of Y = 200.7X + 5.25 (r2 = 0.997). The method proved to be a reliable and effective tool in analysis of anti-oxidant properties in varied tea cultivars and their processed products.In this study, the simple DPPH UV-spectrophotometric analytical procedures were optimized and validated for the estimation of antioxidant properties in aerated, semi-aerated and non-aerated tea products as per the AOAC guidelines. The method was found to be simple, rapid, accurate and economical in estimating antioxidant properties in the different tea products. A (0.0100 ± 0.0001) g well ground tea sample was extracted directly in 80% v/v methanol in distilled water. The precision of the method had an RSD% of 1.695 with a linear relation equation of Y = 200.7X + 5.25 (r2 = 0.997). The method proved to be a reliable and effective tool in analysis of anti-oxidant properties in varied tea cultivars and their processed products.

关 键 词：UV-Spectrophotometric Optimized Validated Antioxidant 

分 类 号：R28[医药卫生—中药学]