Improving Oxygen Binding of Desiccated Human Red Blood Cells  

作　　者：Steingrimur Stefansson David S. Chung Jamie Yoon Won Seok Yoo Young Wook Park George Kim David Hahn Huyen Le Sung-Jae Chung Stephen P. Bruttig David H. Ho Steingrimur Stefansson;David S. Chung;Jamie Yoon;Won Seok Yoo;Young Wook Park;George Kim;David Hahn;Huyen Le;Sung-Jae Chung;Stephen P. Bruttig;David H. Ho(HeMemics Biotechnologies Inc., Rockville, MD, USA;Division of Biology & Medicine, Brown University, Providence, RI, USA;Earl Warren College, UC San Diego, CA, USA;Department of Chemistry, Michigan University, Ann Arbor, MI, USA;Seoul International School, Seongnam, South Korea;Fuzbien Technology Institute, Rockville, MD, USA;Nauah Solutions, Mclean, VA, USA;Marymount University School of Arts & Science, Arlington, VA, USA)

机构地区：[1]HeMemics Biotechnologies Inc., Rockville, MD, USA [2]Division of Biology & Medicine, Brown University, Providence, RI, USA [3]Earl Warren College, UC San Diego, CA, USA [4]Department of Chemistry, Michigan University, Ann Arbor, MI, USA [5]Seoul International School, Seongnam, South Korea [6]Fuzbien Technology Institute, Rockville, MD, USA [7]Nauah Solutions, Mclean, VA, USA [8]Marymount University School of Arts & Science, Arlington, VA, USA

出　　处：《Advances in Bioscience and Biotechnology》2016年第2期47-54,共8页生命科学与技术进展（英文）

摘　　要：Desiccating human red blood cells (RBCs) to increase their storage life has been the subject of intense research for a number of years. However, drying RBCs invariably compromises their integrity and has detrimental effects on hemoglobin function due to autoxidation. We have previously demonstrated an RBC desiccation and rehydration process that preserves RBC antigenic epitopes better than frozen RBCs. This study expands on those observations by examining what effects this desiccation process has on RBC hemoglobin function with respect to oxygen binding properties. In this paper, we examined RBCs from normal donors which were desiccated to 25% moisture content and stored dry for 2 weeks at room temperature prior to rehydration with plasma followed by structural and functional studies. Our data showed that approximately 98% of the RBCs were intact upon rehydration based on hemolysis assays. Oxygen dissociation curves for the desiccated/rehydrated RBCs showed a left shift compared to fresh RBCs (pO2 = 17 mmHg vs. 26 mmHg, respectively). The desiccated/rehydrated RBCs also showed an increase in methemoglobin compared to fresh RBCs (4.5% vs 0.9%, respectively). 2,3-Diphosphoglycerate concentration of the desiccated/rehydrated RBCs was reduced by 20%. In conclusion, although this RBC dehydration process preserves RBC integrity and hemoglobin oxygen binding properties better than most other dehydration techniques described so far, further optimization and long-term studies are needed to make this procedure acceptable for human transfusion.Desiccating human red blood cells (RBCs) to increase their storage life has been the subject of intense research for a number of years. However, drying RBCs invariably compromises their integrity and has detrimental effects on hemoglobin function due to autoxidation. We have previously demonstrated an RBC desiccation and rehydration process that preserves RBC antigenic epitopes better than frozen RBCs. This study expands on those observations by examining what effects this desiccation process has on RBC hemoglobin function with respect to oxygen binding properties. In this paper, we examined RBCs from normal donors which were desiccated to 25% moisture content and stored dry for 2 weeks at room temperature prior to rehydration with plasma followed by structural and functional studies. Our data showed that approximately 98% of the RBCs were intact upon rehydration based on hemolysis assays. Oxygen dissociation curves for the desiccated/rehydrated RBCs showed a left shift compared to fresh RBCs (pO2 = 17 mmHg vs. 26 mmHg, respectively). The desiccated/rehydrated RBCs also showed an increase in methemoglobin compared to fresh RBCs (4.5% vs 0.9%, respectively). 2,3-Diphosphoglycerate concentration of the desiccated/rehydrated RBCs was reduced by 20%. In conclusion, although this RBC dehydration process preserves RBC integrity and hemoglobin oxygen binding properties better than most other dehydration techniques described so far, further optimization and long-term studies are needed to make this procedure acceptable for human transfusion.

关 键 词：DESICCATION Red Blood Cells Oxygen Binding HEMOLYSIS METHEMOGLOBIN 2 3-Diphosphoglycerate 

分 类 号：R87[医药卫生—运动医学]