Molecular Identification and Characterization of a Begomovirus Associated with Okra Enation Leaf Curl Disease in Mali  

作　　者：Gaoussou K. Keita Laya Kansaye Lassina Doumbia Ibrahim Keita Mariam Sangare Tidiani Sinayoko Boubacar Macalou Moussa Noussourou Maiga Nadou Paul Sanogo Ousmane Koita Gaoussou K. Keita;Laya Kansaye;Lassina Doumbia;Ibrahim Keita;Mariam Sangare;Tidiani Sinayoko;Boubacar Macalou;Moussa Noussourou Maiga;Nadou Paul Sanogo;Ousmane Koita(Institut Polytechnique Rural de Formation et de Recherche Appliquée (IPR/IFRA) de Katibougou, Koulikoro, Mali;Université des Sciences, des Techniques et des Technologies de Bamako (USTTB) de Bamako, Laboratoire de Biologie Moléculaire Appliquée (LBMA), Bamako, Mali;Institut Economie Rural (IER), Bamako, Mali)

机构地区：[1]Institut Polytechnique Rural de Formation et de Recherche Appliquée (IPR/IFRA) de Katibougou, Koulikoro, Mali [2]Université des Sciences, des Techniques et des Technologies de Bamako (USTTB) de Bamako, Laboratoire de Biologie Moléculaire Appliquée (LBMA), Bamako, Mali [3]Institut Economie Rural (IER), Bamako, Mali

出　　处：《Advances in Bioscience and Biotechnology》2023年第10期429-438,共10页生命科学与技术进展（英文）

摘　　要：Okra is one of the most widespread vegetable crops in the world, particularly in West Africa. However, several factors influence okra crops as biotic and abiotic factors. Among the diseases affecting its culture, okra leaf curl disease is a major threat. This study aims to assess begomoviruses from okra plants with symptoms like leaves curl by molecular approach. A total of thirteen serologically positive samples were tested by PCR assay, and one sample was sequenced among them. The begomovirus was found in six isolates, Bhendi yellow vein mosaic virus (BYVMV) in three isolates, and Okra enation leaf curl virus (OELCuV) in three isolates, respectively. The begomovirus isolate sequences shared 90% identity with the Cotton leaf curl Gezira virus. Mixed infections between these viruses were found. Thus, these results highlight the need to monitor the spread of these disease-causing viruses for okra crops in Mali. In addition, they can also lead to a considerable loss in okra fields in Koulikoro, which is an agricultural region by excellence.Okra is one of the most widespread vegetable crops in the world, particularly in West Africa. However, several factors influence okra crops as biotic and abiotic factors. Among the diseases affecting its culture, okra leaf curl disease is a major threat. This study aims to assess begomoviruses from okra plants with symptoms like leaves curl by molecular approach. A total of thirteen serologically positive samples were tested by PCR assay, and one sample was sequenced among them. The begomovirus was found in six isolates, Bhendi yellow vein mosaic virus (BYVMV) in three isolates, and Okra enation leaf curl virus (OELCuV) in three isolates, respectively. The begomovirus isolate sequences shared 90% identity with the Cotton leaf curl Gezira virus. Mixed infections between these viruses were found. Thus, these results highlight the need to monitor the spread of these disease-causing viruses for okra crops in Mali. In addition, they can also lead to a considerable loss in okra fields in Koulikoro, which is an agricultural region by excellence.

关 键 词：OKRA PCR BEGOMOVIRUS BYVMV OELCuV MALI 

分 类 号：H33[语言文字—德语]