Microbial Diversity and Key Metabolic Pathways in Lignite-Promoted Anaerobic Fermentation with Residual Sludge  

作　　者：Yawei Zhang Hongyu Guo Daping Xia Shufeng Zhao Ze Deng Dan Huang Bing Li Yinchuan Li Yawei Zhang;Hongyu Guo;Daping Xia;Shufeng Zhao;Ze Deng;Dan Huang;Bing Li;Yinchuan Li(School of Energy Science and Engineering, Henan Polytechnic University, Jiaozuo, China;Collaborative Innovation Center of Coalbed Methane and Shale Gas for Central Plains Economic Region, Jiaozuo, China;Institute of Resources and Environment, Henan Polytechnic University, Jiaozuo, China;Research Institute of Petroleum Exploration & Development, Beijing, China;Yellow River Conservancy Technical Institute, Kaifeng, China)

机构地区：[1]School of Energy Science and Engineering, Henan Polytechnic University, Jiaozuo, China [2]Collaborative Innovation Center of Coalbed Methane and Shale Gas for Central Plains Economic Region, Jiaozuo, China [3]Institute of Resources and Environment, Henan Polytechnic University, Jiaozuo, China [4]Research Institute of Petroleum Exploration & Development, Beijing, China [5]Yellow River Conservancy Technical Institute, Kaifeng, China

出　　处：《Advances in Bioscience and Biotechnology》2024年第11期637-654,共18页生命科学与技术进展（英文）

摘　　要：To enhance methane production efficiency in lignite anaerobic digestion and explore new ways for residual sludge utilization, this study employed the co-fermentation of lignite and residual sludge for biomethane conversion. The bacterial colony structure, metabolic pathways, and interactions between residual sludge and lignite in anaerobic methanogenic fermentation with different mass ratios were analyzed using macrogenomics sequencing. This study aimed to explore the mechanisms involved in the co-anaerobic fermentation of lignite and residual sludge. The results indicated that the addition of sludge enhanced the metabolic pathways in hydrolysis acidification, hydrogen-acetic acid production, and methanation phases. Notably, the enhancement of acetate- and carbon dioxide-nutrient metabolic pathways was more pronounced, with increased activity observed in related enzymes such as acetic acid kinase (k00925) and acetyl coenzyme synthetase (K01895). This increased enzymatic activity facilitated the microbial conversion of biomethane. The results of the study indicated that the sludge exhibited a promotional effect on the methane produced through the anaerobic fermentation of lignite, providing valuable insights for lignite and residual sludge resource utilization.To enhance methane production efficiency in lignite anaerobic digestion and explore new ways for residual sludge utilization, this study employed the co-fermentation of lignite and residual sludge for biomethane conversion. The bacterial colony structure, metabolic pathways, and interactions between residual sludge and lignite in anaerobic methanogenic fermentation with different mass ratios were analyzed using macrogenomics sequencing. This study aimed to explore the mechanisms involved in the co-anaerobic fermentation of lignite and residual sludge. The results indicated that the addition of sludge enhanced the metabolic pathways in hydrolysis acidification, hydrogen-acetic acid production, and methanation phases. Notably, the enhancement of acetate- and carbon dioxide-nutrient metabolic pathways was more pronounced, with increased activity observed in related enzymes such as acetic acid kinase (k00925) and acetyl coenzyme synthetase (K01895). This increased enzymatic activity facilitated the microbial conversion of biomethane. The results of the study indicated that the sludge exhibited a promotional effect on the methane produced through the anaerobic fermentation of lignite, providing valuable insights for lignite and residual sludge resource utilization.

关 键 词：LIGNITE Residual Sludge Anaerobic Fermentation Bacterial Colony Structure Metabolic Pathway 

分 类 号：X70[环境科学与工程—环境工程]