Combinatorial Enzyme Approach to Convert Wheat Insoluble Arabinoxylan to Bioactive Oligosaccharides  

作　　者：Dominic W. S. Wong Sarah Batt William H. Orts Dominic W. S. Wong;Sarah Batt;William H. Orts(Western Regional Research Center, United States Department of Agriculture, Agricultural Research Service, Albany, CA, USA)

机构地区：[1]Western Regional Research Center, United States Department of Agriculture, Agricultural Research Service, Albany, CA, USA

出　　处：《Advances in Enzyme Research》2023年第1期1-10,共10页酶研究进展（英文）

摘　　要：Combinatorial enzyme technology was applied for the conversion of wheat insoluble arabinoxylan to oligosaccharide structural variants. The digestive products were fractionated by Bio-Gel P4 column and screened for bioactivity. One fraction pool was observed to exhibit antimicrobial property resulting in the suppression of cell growth of the test organism ATCC 8739 E. coli. It has a MIC value of 1.5% (w/v, 35°C, 20 hr) and could be useful as a new source of prebiotics or preservatives. The present results further confirm the science and useful application of combinatorial enzyme approach.Combinatorial enzyme technology was applied for the conversion of wheat insoluble arabinoxylan to oligosaccharide structural variants. The digestive products were fractionated by Bio-Gel P4 column and screened for bioactivity. One fraction pool was observed to exhibit antimicrobial property resulting in the suppression of cell growth of the test organism ATCC 8739 E. coli. It has a MIC value of 1.5% (w/v, 35°C, 20 hr) and could be useful as a new source of prebiotics or preservatives. The present results further confirm the science and useful application of combinatorial enzyme approach.

关 键 词：Combinatorial Enzyme Approach Wheat Insoluble Arabinoxylan Bioactive Oligosaccharides 

分 类 号：S51[农业科学—作物学]