Sensitivity and Specificity of Various Serological Tests for Detection of Brucella spp. Infection in Male Goats and Sheep  

作　　者：Waffa A. Ahmed Shaimaa A. Majeed Ameer H. Abdul Ameer Nawal D. Mahmmod Nameer I. Saeed Luma. Y. Hanaa Waffa A. Ahmed;Shaimaa A. Majeed;Ameer H. Abdul Ameer;Nawal D. Mahmmod;Nameer I. Saeed;Luma. Y. Hanaa(Zoonosis Diseases Unit, College of Veterinary Medicine, Baghdad University, Baghdad, Iraq;Ministery of Agriculture, Agriculture Research Office, Baghdad, Iraq;Vet Research & Laboratories Department, Baghdad, Iraq)

机构地区：[1]Zoonosis Diseases Unit, College of Veterinary Medicine, Baghdad University, Baghdad, Iraq [2]Ministery of Agriculture, Agriculture Research Office, Baghdad, Iraq [3]Vet Research & Laboratories Department, Baghdad, Iraq

出　　处：《Advances in Microbiology》2016年第2期98-103,共6页微生物学（英文）

摘　　要：Brucellosis is a zoonosis found throughout the world with major implications both in the field of public health and farming economy. It is most often caused by Brucella abortus and Brucella melitensis. This study aimed for evaluation of the sensitivity and specificity of immunochromatographic assay (ICA) and comparing the results with those obtained from Rose Bengal Plate Test (RBPT) and in direct Enzyme Linked Immunsorbent Assay (ELISA) for serodiagnosis of sheep and goat brucellosis. Out of 41 sera samples (21 ram and 20 male goats) which were examined, antibodies against Brucella were detected in ram 8 (38.09%), 6 (28.57%) and 16 (76.19%) in ram samples, whereas they were detected in 3 (15%), 1 (5%) and 12 (60%) serum sample of goats males by RBPT, (ICA) and iELISA respectively. The kappa value between iELISA and ICA was -0.08 and that between RBPT and ELISA was -0.01 in ram and the kappa value between iELISA and ICA was -0.06 and that between RBPT and ELISA was -0.14 in male goats. The sensitivity for RBPT and ICA were 37.5% and 25% while specificity was 60% and 60% in ram;whereas, in male goats, sensitivity for RBPT was 8.33% and ICA was 8.33% while specificity was 75% and 100% respectively. It can be concluded that the developed ICA is immunodiagnostic assay, and it is rapid, non-expensive, economical and suitable for large-scale screening in developing countries and rural areas.Brucellosis is a zoonosis found throughout the world with major implications both in the field of public health and farming economy. It is most often caused by Brucella abortus and Brucella melitensis. This study aimed for evaluation of the sensitivity and specificity of immunochromatographic assay (ICA) and comparing the results with those obtained from Rose Bengal Plate Test (RBPT) and in direct Enzyme Linked Immunsorbent Assay (ELISA) for serodiagnosis of sheep and goat brucellosis. Out of 41 sera samples (21 ram and 20 male goats) which were examined, antibodies against Brucella were detected in ram 8 (38.09%), 6 (28.57%) and 16 (76.19%) in ram samples, whereas they were detected in 3 (15%), 1 (5%) and 12 (60%) serum sample of goats males by RBPT, (ICA) and iELISA respectively. The kappa value between iELISA and ICA was -0.08 and that between RBPT and ELISA was -0.01 in ram and the kappa value between iELISA and ICA was -0.06 and that between RBPT and ELISA was -0.14 in male goats. The sensitivity for RBPT and ICA were 37.5% and 25% while specificity was 60% and 60% in ram;whereas, in male goats, sensitivity for RBPT was 8.33% and ICA was 8.33% while specificity was 75% and 100% respectively. It can be concluded that the developed ICA is immunodiagnostic assay, and it is rapid, non-expensive, economical and suitable for large-scale screening in developing countries and rural areas.

关 键 词：BRUCELLOSIS RBPT ICA IELISA SHEEP GOAT 

分 类 号：R51[医药卫生—内科学]