Prevalence of CTXM, SHV, TEM AND OXA Genes among Extended-Spectrum Beta-Lactamase Producing <i>Klebsiella pneumoniae</i>from Mukuru Slum, Kenya  

作　　者：Hellen Saisi[1] Celestine Makobe[1] Maureen Kangongo[1] Samwuel Kariuki[1] 

出　　处：《Advances in Microbiology》2019年第10期853-862,共10页微生物学（英文）

摘　　要：Background: Extended spectrum beta lactamases (ESBLs) producing Enterobacteriaceae cause infections that are often reported in both hospital and community setting. These infections are on the increase and jeopardize the achievement of modern medicine because of their clinical implications. There is need for surveillance measures to be taken, both by the health care personnel and the community at large. Methodology: We examined 330 diarrhea stool samples from children below the age of 5 years and processed them. A total of 96 (29%) samples were identified as Klebsiella pneumoniae out of the bacteria isolated. Identification of ESBL was done and 42 K. pneumoniae isolates were tested for the occurrence of blaCTX-M, blaOXA, blaTEM and blaSHV resistant genes by PCR, gel electrophoresis and visualized by UV illumination. Results: Our results revealed that blaCTXM was the most frequent ESBL type 42 (100%), followed by blaTEM in 41 (97.6%) isolates and blaSHVin38 (90.4%) of the isolates. None of the tested isolates were found to be encoding blaOXA. There was occurrence of more than one gene in most of the isolates. The double combination was detected in blaCTX-M/blaTEM (9.5%) and blaCTXM/SHV (2.4%). A triple combination was noted blaTEM/blaSHV/bla CTX-M (88%). Conclusion: Our results indicate that there is Presence of Beta lactam genes associated with antimicrobial resistance among the K. pneumoniae isolates from Mukuru Slum, Kenya. The predominant ESBL genotype in Mukuru slums, Kenya was blaCTX-M followed by blaTEM and blaSHV respectively. There is need for surveillance measures to be taken so as to control the spread among the community.Background: Extended spectrum beta lactamases (ESBLs) producing Enterobacteriaceae cause infections that are often reported in both hospital and community setting. These infections are on the increase and jeopardize the achievement of modern medicine because of their clinical implications. There is need for surveillance measures to be taken, both by the health care personnel and the community at large. Methodology: We examined 330 diarrhea stool samples from children below the age of 5 years and processed them. A total of 96 (29%) samples were identified as Klebsiella pneumoniae out of the bacteria isolated. Identification of ESBL was done and 42 K. pneumoniae isolates were tested for the occurrence of blaCTX-M, blaOXA, blaTEM and blaSHV resistant genes by PCR, gel electrophoresis and visualized by UV illumination. Results: Our results revealed that blaCTXM was the most frequent ESBL type 42 (100%), followed by blaTEM in 41 (97.6%) isolates and blaSHVin38 (90.4%) of the isolates. None of the tested isolates were found to be encoding blaOXA. There was occurrence of more than one gene in most of the isolates. The double combination was detected in blaCTX-M/blaTEM (9.5%) and blaCTXM/SHV (2.4%). A triple combination was noted blaTEM/blaSHV/bla CTX-M (88%). Conclusion: Our results indicate that there is Presence of Beta lactam genes associated with antimicrobial resistance among the K. pneumoniae isolates from Mukuru Slum, Kenya. The predominant ESBL genotype in Mukuru slums, Kenya was blaCTX-M followed by blaTEM and blaSHV respectively. There is need for surveillance measures to be taken so as to control the spread among the community.

关 键 词：Klebsiella PNEUMONIAE Resistant GENES ESBL 

分 类 号：R44[医药卫生—诊断学]