Antimicrobial Activity of a Formulation of <i>Libidibia ferrea</i>L. against Microorganisms of the Dental Biofilm  

作　　者：Raquel de Oliveira Marreiro Maria Fulgência Costa Lima Bandeira Carina Toda Fábio Correia Sampaio Tatiane Pereira de Souza Gisely Naura Venâncio Wanderleia Monteiro de Souza Nikeila Chacon de Oliveira Conde Raquel de Oliveira Marreiro;Maria Fulgência Costa Lima Bandeira;Carina Toda;Fábio Correia Sampaio;Tatiane Pereira de Souza;Gisely Naura Venâncio;Wanderleia Monteiro de Souza;Nikeila Chacon de Oliveira Conde(School of Dentistry, Federal University of Amazonas, Manaus, AM, Brazil;Federal University of Paraíba, Health Sciences Center, Department of Clinical and Social Dentistry, João Pessoa, PB, Brazil;Faculty of Pharmaceutical Sciences, Federal University of Amazonas, Manaus, AM, Brazil;Manaus Municipal Health Department, SEMSA, Manaus, AM, Brazil)

机构地区：[1]School of Dentistry, Federal University of Amazonas, Manaus, AM, Brazil [2]Federal University of Paraíba, Health Sciences Center, Department of Clinical and Social Dentistry, Joã o Pessoa, PB, Brazil [3]Faculty of Pharmaceutical Sciences, Federal University of Amazonas, Manaus, AM, Brazil [4]Manaus Municipal Health Department, SEMSA, Manaus, AM, Brazil

出　　处：《Advances in Microbiology》2020年第9期434-442,共9页微生物学（英文）

摘　　要：This study aimed to evaluate the antimicrobial activity of the extract of <i>Libidibia ferrea</i> L. (228.022-INPA) and a mouthwash formulation against microorganisms of the dental biofilm. The extract of <i>Libidibia ferrea</i> L. was prepared and formulated with an alcohol-free mouthwash. The Minimum Inhibitory Concentration—MIC was determined by microdilution and cell viability was evaluated through fluorescence. The MICs for the extract were 4.375 μg/mL (<i>Streptococcus mutans</i>), 3.750 μg/mL (<i>Streptococcus oralis</i>), 4.375 μg/mL (<i>Lactobacillus casei</i>). In contrast, for the mouthwash, the MICs were 6.000 μg/mL (bacteriostatic against <i>Streptococcus mutans</i> and bactericidal against <i>Streptococcus oralis</i>). In 1.250 μg/mL of the extract, the number of viable cells measured by fluorescence tests was 16.47% and 14.12% for <i>Streptococcus mutans</i> and <i>Lactobacillus casei</i>, respectively. For the mouthwash (1.000 μg/mL), 20.60% of viable cells for <i>Streptococcus mutans</i>, and 9.60% for <i>Lactobacillus casei</i> were observed. It can be concluded that the extract and its mouthwash containig <i>Libidibia ferrea</i> L. showed antibacterial activity against microorganisms from the dental biofilm.This study aimed to evaluate the antimicrobial activity of the extract of <i>Libidibia ferrea</i> L. (228.022-INPA) and a mouthwash formulation against microorganisms of the dental biofilm. The extract of <i>Libidibia ferrea</i> L. was prepared and formulated with an alcohol-free mouthwash. The Minimum Inhibitory Concentration—MIC was determined by microdilution and cell viability was evaluated through fluorescence. The MICs for the extract were 4.375 μg/mL (<i>Streptococcus mutans</i>), 3.750 μg/mL (<i>Streptococcus oralis</i>), 4.375 μg/mL (<i>Lactobacillus casei</i>). In contrast, for the mouthwash, the MICs were 6.000 μg/mL (bacteriostatic against <i>Streptococcus mutans</i> and bactericidal against <i>Streptococcus oralis</i>). In 1.250 μg/mL of the extract, the number of viable cells measured by fluorescence tests was 16.47% and 14.12% for <i>Streptococcus mutans</i> and <i>Lactobacillus casei</i>, respectively. For the mouthwash (1.000 μg/mL), 20.60% of viable cells for <i>Streptococcus mutans</i>, and 9.60% for <i>Lactobacillus casei</i> were observed. It can be concluded that the extract and its mouthwash containig <i>Libidibia ferrea</i> L. showed antibacterial activity against microorganisms from the dental biofilm.

关 键 词：MOUTHWASH Juca Natural Products 

分 类 号：R78[医药卫生—口腔医学]