The Antiviral Efficacy of <i>Withania somnifera</i>(Ashwagandha) against Hepatitis C Virus Activity: <i>In Vitro</i>and <i>in Silico</i>Study  

作　　者：Dina Mofed Wafaa Ahmed Abdel-Rahman Zekri Ola Said Mohamed Rahouma Ahmed Hassan Ibrahim Faraag Dina Mofed;Wafaa Ahmed;Abdel-Rahman Zekri;Ola Said;Mohamed Rahouma;Ahmed Hassan Ibrahim Faraag(Biochemistry and Molecular Biology Unit, Department of Cancer Biology, National Cancer Institute, Cairo University, Cairo, Egypt;Immunity and Virology Unit, Department of Cancer Biology, National Cancer Institute, Cairo University, Cairo, Egypt;Department of Surgical Oncology, National Cancer Institute, Cairo University, Cairo, Egypt;Department of Botany and Microbiology, Faculty of Science, Helwan University, Cairo, Egypt)

机构地区：[1]Biochemistry and Molecular Biology Unit, Department of Cancer Biology, National Cancer Institute, Cairo University, Cairo, Egypt [2]Immunity and Virology Unit, Department of Cancer Biology, National Cancer Institute, Cairo University, Cairo, Egypt [3]Department of Surgical Oncology, National Cancer Institute, Cairo University, Cairo, Egypt [4]Department of Botany and Microbiology, Faculty of Science, Helwan University, Cairo, Egypt

出　　处：《Advances in Microbiology》2020年第9期463-477,共15页微生物学（英文）

摘　　要：<b>Objective:</b> Evaluation antiviral effects of <i>Withania somnifera</i> (Ashwagandha) leaf extract against HCV. <b>Methods:</b> cell proliferation was assessed using MTT assay after isolation of lymphocyte cells and treated with Ashwagandha water extract (ASH-WX) (6.25 mg/ml - 100 mg/ml). Assessment of quantitative Real-time PCR, Colony forming assay, TNF-<i>α</i> and molecular docking studies after infection of normal lymphocyte cells with 1 ml (1.5 × 10⁶ HCV) serum then incubated with ASH-WX at concentration 25 mg/ml & 50 mg/ml. <b>Results:</b> MTT assay revealed a significant increase (p < 0.001) in normal lymphocyte proliferation at all concentration’s particularity at 25 mg/ml with SI (6.06) and at 50 mg/ml with (5.8). While TNF-<i>α</i> significantly decreased following ASH-WX treatment compared with control untreated infected cells (p < 0.05). PCR results showed a marked viral load reduction after treatment by ASH-WX at concentration 25 mg/ml to 6.241 × 10³ IU/mL. Colony formation assay test revealed colony formation reduction compared to positive untreated control. Molecular docking analysis revealed good prediction of binding between Ashwagandha and NS5B and PKN2 compared to Sovaldi. <b>Conclusion: </b> ASH-WX may be a powerful antiviral against HCV infection.<b>Objective:</b> Evaluation antiviral effects of <i>Withania somnifera</i> (Ashwagandha) leaf extract against HCV. <b>Methods:</b> cell proliferation was assessed using MTT assay after isolation of lymphocyte cells and treated with Ashwagandha water extract (ASH-WX) (6.25 mg/ml - 100 mg/ml). Assessment of quantitative Real-time PCR, Colony forming assay, TNF-<i>α</i> and molecular docking studies after infection of normal lymphocyte cells with 1 ml (1.5 × 10⁶ HCV) serum then incubated with ASH-WX at concentration 25 mg/ml & 50 mg/ml. <b>Results:</b> MTT assay revealed a significant increase (p < 0.001) in normal lymphocyte proliferation at all concentration’s particularity at 25 mg/ml with SI (6.06) and at 50 mg/ml with (5.8). While TNF-<i>α</i> significantly decreased following ASH-WX treatment compared with control untreated infected cells (p < 0.05). PCR results showed a marked viral load reduction after treatment by ASH-WX at concentration 25 mg/ml to 6.241 × 10³ IU/mL. Colony formation assay test revealed colony formation reduction compared to positive untreated control. Molecular docking analysis revealed good prediction of binding between Ashwagandha and NS5B and PKN2 compared to Sovaldi. <b>Conclusion: </b> ASH-WX may be a powerful antiviral against HCV infection.

关 键 词：ANTIVIRAL ASHWAGANDHA Hepatitis C Virus Docking LYMPHOCYTE 

分 类 号：R51[医药卫生—内科学]