A Retrospective Study on the Pyogenic Pathogens and Their Antibiotic Susceptibility Patterns along with the ES<i>β</i>L Production  

作　　者：Anindita Chakraborty Debadatta Dhar Chanda Nilanjana Choudhury N. G. Manjula B. M. Shilpa Anindita Chakraborty;Debadatta Dhar Chanda;Nilanjana Choudhury;N. G. Manjula;B. M. Shilpa(Department of Microbiology, School of Basic and Applied Sciences (SBAS), Dayananda Sagar University, Bengaluru, Karnataka;Department of Microbiology, Silchar Medical College and Hospital, Assam, India)

机构地区：[1]Department of Microbiology, School of Basic and Applied Sciences (SBAS), Dayananda Sagar University, Bengaluru, Karnataka [2]Department of Microbiology, Silchar Medical College and Hospital, Assam, India

出　　处：《Advances in Microbiology》2021年第6期317-326,共10页微生物学（英文）

摘　　要：Pyogenic infections are caused by various pathogens leading to pus formation and that can be attributed due to a wound either through accident or during surgery leading to infection spread. There are pathogenic strains that are not uncommon in hospital settings like <i>Staphylococcus aureus</i>, <i>Klebsiella pneumoniae</i>, <i>Pseudomonas aeruginosa</i>, <i>Acinetobacter</i> etc., that are multidrug resistant (MDR) and are a cause of concern. The bacteriological profile in the pyogenic infections tends to be same but there is a vast difference with the antibiotic resistant patterns in different hospital settings. Hence, the aim was to study the antibiotic susceptibility profiles and Extended spectrum <i>β</i>eta Lactamases (ES<i>β</i>L) production in these pathogens. A prospective study was carried out in Silchar Medical College and Hospital Assam, India, over a four-month period from February to May 2021. The samples were processed using Blood and MacConkey’s agar. Further, these isolated pathogens were identified by standard morphological, cultural and biochemical tests. The antibiotic susceptibility test was conducted by Kirby Bauer disc diffusion method and ES<i>β</i>L production was detected by using combined disk diffusion test. It was observed that the identified pathogens had an incidence rate of 84.2% and further revealed that Gram negative had a higher incidence rate compared to Gram positive with 59.8%. The pathogens isolated from pus samples had a maximum of <i>Klebsiella sps</i> (19.64%) and the lowest was <i>E. coli</i> with 5.36%. Antibiotic susceptibility test (AST) of Gram-negative bacterial isolates showed the highest incidence with aztreonam (40.6%) and the lowest was observed in Piperacillin/Tazobactam with 7.5%. The only Gram positive was observed in our study, <i>Staphylococcus aureus</i> had the highest resistance in amikacin with 80% and interestingly, all the isolates were sensitive to Linezolid with 100%. There is a high rise and spreading with the multi-drug resistance (MDR) straiPyogenic infections are caused by various pathogens leading to pus formation and that can be attributed due to a wound either through accident or during surgery leading to infection spread. There are pathogenic strains that are not uncommon in hospital settings like <i>Staphylococcus aureus</i>, <i>Klebsiella pneumoniae</i>, <i>Pseudomonas aeruginosa</i>, <i>Acinetobacter</i> etc., that are multidrug resistant (MDR) and are a cause of concern. The bacteriological profile in the pyogenic infections tends to be same but there is a vast difference with the antibiotic resistant patterns in different hospital settings. Hence, the aim was to study the antibiotic susceptibility profiles and Extended spectrum <i>β</i>eta Lactamases (ES<i>β</i>L) production in these pathogens. A prospective study was carried out in Silchar Medical College and Hospital Assam, India, over a four-month period from February to May 2021. The samples were processed using Blood and MacConkey’s agar. Further, these isolated pathogens were identified by standard morphological, cultural and biochemical tests. The antibiotic susceptibility test was conducted by Kirby Bauer disc diffusion method and ES<i>β</i>L production was detected by using combined disk diffusion test. It was observed that the identified pathogens had an incidence rate of 84.2% and further revealed that Gram negative had a higher incidence rate compared to Gram positive with 59.8%. The pathogens isolated from pus samples had a maximum of <i>Klebsiella sps</i> (19.64%) and the lowest was <i>E. coli</i> with 5.36%. Antibiotic susceptibility test (AST) of Gram-negative bacterial isolates showed the highest incidence with aztreonam (40.6%) and the lowest was observed in Piperacillin/Tazobactam with 7.5%. The only Gram positive was observed in our study, <i>Staphylococcus aureus</i> had the highest resistance in amikacin with 80% and interestingly, all the isolates were sensitive to Linezolid with 100%. There is a high rise and spreading with the multi-drug resistance (MDR) strai

关 键 词：Antibiotic Susceptibility Test PUS ESβL MDR 

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