Efficacy of Beneficial Fungi Isolates in Solanum lycopersicum L. Protection against Lepidopteran Insects through a Leaf Inoculation Technique  

作　　者：Joelle Toffa Elie Ayitondji Dannon Yeyinou Laura Estelle Loko Hervé Bokossa Josky Adikpeto Appolinaire Adandonon Manuele Tamò Joelle Toffa;Elie Ayitondji Dannon;Yeyinou Laura Estelle Loko;Hervé Bokossa;Josky Adikpeto;Appolinaire Adandonon;Manuele Tamò(International Institute of Tropical Agriculture (IITA), Tri Postal, Cotonou, Bénin;Laboratoire d’Entomologie Appliquée, Ecole Nationale Supérieure des Biosciences et Biotechnologies Appliquées/Université;Laboratoire d’Entomologie Appliquée, Ecole Nationale Supérieure des Biosciences et Biotechnologies Appliquées/Université Nationale des Sciences, Technologies, Ingénierie et Mathématiques d’Abomey au Bénin, Dassa, Bénin;University of Abomey-Calavi (UAC), Calavi, Bénin;National University of Agriculture (UNA), Ketou, Benin)

机构地区：[1]International Institute of Tropical Agriculture (IITA), Tri Postal, Cotonou, Bénin [2]Laboratoire d’Entomologie Appliquée, Ecole Nationale Supérieure des Biosciences et Biotechnologies Appliquées/Université [3]Laboratoire d’Entomologie Appliquée, Ecole Nationale Supérieure des Biosciences et Biotechnologies Appliquées/Université Nationale des Sciences, Technologies, Ingénierie et Mathématiques d’Abomey au Bénin, Dassa, Bénin [4]University of Abomey-Calavi (UAC), Calavi, Bénin [5]National University of Agriculture (UNA), Ketou, Benin

出　　处：《Advances in Microbiology》2023年第2期89-105,共17页微生物学（英文）

摘　　要：Helicoverpa armigera is a key insect pest of tomatoes reducing drastically yields. The effect of the endophytic colonization of tomato plants by Beauveria bassiana using leaf spray as an inoculation method on damage and survival of H. armigera was assessed in a screen house. Two B. bassiana isolates (Bb 115 and Bb 11) and two tomato varieties (a local variety Tounvi and an improved variety Padma) were included in the study. The adaxial and abaxial leaf surfaces were sprayed at a concentration of 10⁷ conidia/ml and 10⁹ conidia/ml for each isolate and each of the two tomato varieties. Thirty days after inoculation, five discs of tomato leaf and tomato root were cut for each isolate, each concentration per isolate and for each variety. The samples were incubated at room temperature (28°C ± 2°C) and periodically checked for fungal growth. Larval survival was checked and a damage assessment was done on tomato flowers and the leaves. The results show that the lowest Mean Survival Times (MSTs) were recorded on larvae feeding on plants inoculated with Bb 11 (4.2 ± 0.8 days against 11.5 ± 0.2 days for control). Compared to the other treatments, low damage rates of the flowers of the improved variety inoculated with Bb 11 at 10⁹ conidia/ml were recorded from the 6th Day After Inoculation (DAI). This rate remains low until the end of treatment. Overall flower damage was lower than leaf damage. The results showed large differences in pathogenicity, with most endophytic isolate belonging to Bb 11 when inoculated at 10⁹ conidia/ml using the leaf spraying technique. Data were discussed with regard to the use of endophytism B. bassiana in an integrated tomato pest control approach.Helicoverpa armigera is a key insect pest of tomatoes reducing drastically yields. The effect of the endophytic colonization of tomato plants by Beauveria bassiana using leaf spray as an inoculation method on damage and survival of H. armigera was assessed in a screen house. Two B. bassiana isolates (Bb 115 and Bb 11) and two tomato varieties (a local variety Tounvi and an improved variety Padma) were included in the study. The adaxial and abaxial leaf surfaces were sprayed at a concentration of 10⁷ conidia/ml and 10⁹ conidia/ml for each isolate and each of the two tomato varieties. Thirty days after inoculation, five discs of tomato leaf and tomato root were cut for each isolate, each concentration per isolate and for each variety. The samples were incubated at room temperature (28°C ± 2°C) and periodically checked for fungal growth. Larval survival was checked and a damage assessment was done on tomato flowers and the leaves. The results show that the lowest Mean Survival Times (MSTs) were recorded on larvae feeding on plants inoculated with Bb 11 (4.2 ± 0.8 days against 11.5 ± 0.2 days for control). Compared to the other treatments, low damage rates of the flowers of the improved variety inoculated with Bb 11 at 10⁹ conidia/ml were recorded from the 6th Day After Inoculation (DAI). This rate remains low until the end of treatment. Overall flower damage was lower than leaf damage. The results showed large differences in pathogenicity, with most endophytic isolate belonging to Bb 11 when inoculated at 10⁹ conidia/ml using the leaf spraying technique. Data were discussed with regard to the use of endophytism B. bassiana in an integrated tomato pest control approach.

关 键 词：Tomato INSECT Beauveria bassiana Foliar Spray ENDOPHYTIC Pest Management 

分 类 号：S64[农业科学—蔬菜学]