Resistance Profile of Urogenital Mycoplasmas to Antibiotics: Comparative Study between the Mycoplasma IST2 and Mycoplasma IST3  

作　　者：Laurent Akono Cedric Gueguim Marie Julie Manga Sipewa Serge Damase Oyong Assiene Marius Noubi Fezeu Laetitia Pascal Eboa Celestin Roger Ayangma Lucien Honore Etame Sone Laurent Akono;Cedric Gueguim;Marie Julie Manga Sipewa;Serge Damase Oyong Assiene;Marius Noubi Fezeu;Laetitia Pascal Eboa;Celestin Roger Ayangma;Lucien Honore Etame Sone(Department of Biology, Higher Institute of Medical Technology, Yaounde, Cameroon;Department of Environmental Sciences, Higher Institute of Agriculture, Wood, Water and the Environment, University of Bertoua, Belabo, Cameroon;Pharmacy Laboratory Galenics and Pharmaceutical Legislation, FMSB, University of Yaound 1, Yaounde, Cameroon;Faculty of Medicine and Pharmaceutical Sciences, University of Douala, Douala, Cameroon;Institute of Medical Research and Medicinal Plants Studies, Yaounde, Cameroon)

机构地区：[1]Department of Biology, Higher Institute of Medical Technology, Yaounde, Cameroon [2]Department of Environmental Sciences, Higher Institute of Agriculture, Wood, Water and the Environment, University of Bertoua, Belabo, Cameroon [3]Pharmacy Laboratory Galenics and Pharmaceutical Legislation, FMSB, University of Yaound 1, Yaounde, Cameroon [4]Faculty of Medicine and Pharmaceutical Sciences, University of Douala, Douala, Cameroon [5]Institute of Medical Research and Medicinal Plants Studies, Yaounde, Cameroon

出　　处：《Advances in Microbiology》2024年第11期578-588,共11页微生物学（英文）

摘　　要：Acquired resistance of mycoplasmas to antibiotics constitutes a major health problem in the world in general and in Africa in particular. Despite the diversity of kits marketed, several of them evaluating the sensitivity of mycoplasmas to antibiotics continue to present resistance, in this case, the Mycoplasma IST2. In order to overcome this resistance, a new kit (Mycoplasma IST3) has been developed in accordance with the new Clinical and Laboratory Standard Institute (CLSI) recommendations. The objective of the study was to determine the resistance profile of urogenital Mycoplasmas to antibiotics using this new kit and to highlight cases of co-infections in comparison with the Mycoplasma IST2. Over a period of four mo nths, one hundred and one (101) samples of urogenital secretions were collected (from sexually active men and women) and analyzed. Culture and antibiotic susceptibility testing were performed in a liquid medium using the Mycoplasma IST2 and Mycoplasma IST3 Tests according to the manufacturer’s recommendations. Among the different samples analyzed, we noted a mycoplasma positivity rate of 71.29% with a predominance of infection in women, i.e. 86.12% compared to men 13.88%. Ureaplasma spp was the most encountered germ with a rate of 62.50% followed by cases of co-infections at 33.33% (Uspp/Mh) and the least encountered was Mycoplasma hominis with a rate of 2.79%. We analyzed 25 samples, among which we had 4 co-infections, simultaneously using the two kits in the same patients. The distribution of cases between the two kits was equivalent. We noted a significant rate of resistance to erythromycin 100% using Mycoplasma IST2. However, no resistance was observed in erythromycin with Mycoplasma IST3. Mycoplasma IST2 also showed resistance to fluoroquinolones, which was not the case for Mycoplasma IST3 which did not show any resistance to fluoroquinolones. Both kits showed resistance to tetracycline. The antibiotic sensitivity test using the Mycoplasma IST3 revealed a high rate of resistance to tetracyclAcquired resistance of mycoplasmas to antibiotics constitutes a major health problem in the world in general and in Africa in particular. Despite the diversity of kits marketed, several of them evaluating the sensitivity of mycoplasmas to antibiotics continue to present resistance, in this case, the Mycoplasma IST2. In order to overcome this resistance, a new kit (Mycoplasma IST3) has been developed in accordance with the new Clinical and Laboratory Standard Institute (CLSI) recommendations. The objective of the study was to determine the resistance profile of urogenital Mycoplasmas to antibiotics using this new kit and to highlight cases of co-infections in comparison with the Mycoplasma IST2. Over a period of four mo nths, one hundred and one (101) samples of urogenital secretions were collected (from sexually active men and women) and analyzed. Culture and antibiotic susceptibility testing were performed in a liquid medium using the Mycoplasma IST2 and Mycoplasma IST3 Tests according to the manufacturer’s recommendations. Among the different samples analyzed, we noted a mycoplasma positivity rate of 71.29% with a predominance of infection in women, i.e. 86.12% compared to men 13.88%. Ureaplasma spp was the most encountered germ with a rate of 62.50% followed by cases of co-infections at 33.33% (Uspp/Mh) and the least encountered was Mycoplasma hominis with a rate of 2.79%. We analyzed 25 samples, among which we had 4 co-infections, simultaneously using the two kits in the same patients. The distribution of cases between the two kits was equivalent. We noted a significant rate of resistance to erythromycin 100% using Mycoplasma IST2. However, no resistance was observed in erythromycin with Mycoplasma IST3. Mycoplasma IST2 also showed resistance to fluoroquinolones, which was not the case for Mycoplasma IST3 which did not show any resistance to fluoroquinolones. Both kits showed resistance to tetracycline. The antibiotic sensitivity test using the Mycoplasma IST3 revealed a high rate of resistance to tetracycl

关 键 词：Urogenital Infection Mycoplasma IST2 Mycoplasma IST3 Antibiotic Resistance 

分 类 号：R69[医药卫生—泌尿科学]