Phenotypic and Genotypic Characterization of mecA Gene in Methicillin Resistance Staphylococcus aureus Isolated from Smoked Fish  

作　　者：Kouame N’Zebo Desiré Benie Comoé Koffi Donatien Atobla Koua Toure Naka Traore Adjaratou Tuo Wako-Tianwa Alice Karou Tago Germain Dadie Adjehi Mireille Dosso Kouame N’Zebo Desiré;Benie Comoé Koffi Donatien;Atobla Koua;Toure Naka;Traore Adjaratou;Tuo Wako-Tianwa Alice;Karou Tago Germain;Dadie Adjehi;Mireille Dosso(Laboratory of Biotechnology, Agriculture and Valuation of Biological Resources, Department of Biosciences, Felix Houphouet-Boigny University, Abidjan, Cte dIvoire;Department of Bacteriology and Virology, National Center Reference for Antibiotics, Pasteur Institute of Cte dIvoire (IPCI), Abidjan, Cte dIvoire;Laboratory of Biotechnology and Valuation of Agri-Resources and Natural Substances, Department of Biological Sciences of Pelefero Gon Coulibaly University, Korhogo, Cte dIvoire;Department of Medical Sciences, University of Alassane Ouattara, University Hospital Center (UHC) of Bouak, Bouak, Cte dIvoire;Laboratory of Microbiology and Biotechnology, Department of Sciences et Technologies for Foods, Nangui Abrogoua University, Abidjan, Cte dIvoire)

机构地区：[1]Laboratory of Biotechnology, Agriculture and Valuation of Biological Resources, Department of Biosciences, Felix Houphouet-Boigny University, Abidjan, Cte dIvoire [2]Department of Bacteriology and Virology, National Center Reference for Antibiotics, Pasteur Institute of Cte dIvoire (IPCI), Abidjan, Cte dIvoire [3]Laboratory of Biotechnology and Valuation of Agri-Resources and Natural Substances, Department of Biological Sciences of Pelefero Gon Coulibaly University, Korhogo, Cte dIvoire [4]Department of Medical Sciences, University of Alassane Ouattara, University Hospital Center (UHC) of Bouak, Bouak, Cte dIvoire [5]Laboratory of Microbiology and Biotechnology, Department of Sciences et Technologies for Foods, Nangui Abrogoua University, Abidjan, Cte dIvoire

出　　处：《Advances in Microbiology》2024年第12期605-617,共13页微生物学(英文)

摘　　要：Staphylococcus aureus is a bacterial species responsible for food poisoning and outbreaks of opportunistic, nosocomial and community-acquired diseases. The aim of this study was to characterize S. aureus strains resistant to methicillin. Seventy-five (75) samples of smoked fish, including Scomber scombrus, Trachurus trachurus, Thunnus spp., Cyprinus spp. and Sardinella spp., were studied. The Mueller-Hinton diffusion method was used to determine the phenotypic resistance profile. The coagulase test and thermonuclease detection were used to assess the enzymatic production potential of the strains. The methicillin resistance mecA gene was detected by PCR. With a contamination rate of 80%, the prevalence of S. aureus varied from 15% to 31.7% in animal products. S. aureus strains were DNase (91.7%) and coagulase (50%) producers. The resistance of these strains was 42.7% (cefoxitin), 37.8% (oxacillin) and 26.4% (cefuroxime sodium). They were resistant to tetracycline (62.4%), erythromycin (61.1%), vancomycin (34.6%), levofloxacin (33.3%) and imipenem (12.7%). The prevalence of the mecA gene in animal products ranged from 13.9% to 33.4%. The mecA gene induction showed different sensitivities with cefoxitin (100%) and oxacillin (56.7%). In addition, all tests showed a specificity of 100%. This work demonstrates the need to strengthen surveillance to prevent the spread of S. aureus epidemics in various environments.Staphylococcus aureus is a bacterial species responsible for food poisoning and outbreaks of opportunistic, nosocomial and community-acquired diseases. The aim of this study was to characterize S. aureus strains resistant to methicillin. Seventy-five (75) samples of smoked fish, including Scomber scombrus, Trachurus trachurus, Thunnus spp., Cyprinus spp. and Sardinella spp., were studied. The Mueller-Hinton diffusion method was used to determine the phenotypic resistance profile. The coagulase test and thermonuclease detection were used to assess the enzymatic production potential of the strains. The methicillin resistance mecA gene was detected by PCR. With a contamination rate of 80%, the prevalence of S. aureus varied from 15% to 31.7% in animal products. S. aureus strains were DNase (91.7%) and coagulase (50%) producers. The resistance of these strains was 42.7% (cefoxitin), 37.8% (oxacillin) and 26.4% (cefuroxime sodium). They were resistant to tetracycline (62.4%), erythromycin (61.1%), vancomycin (34.6%), levofloxacin (33.3%) and imipenem (12.7%). The prevalence of the mecA gene in animal products ranged from 13.9% to 33.4%. The mecA gene induction showed different sensitivities with cefoxitin (100%) and oxacillin (56.7%). In addition, all tests showed a specificity of 100%. This work demonstrates the need to strengthen surveillance to prevent the spread of S. aureus epidemics in various environments.

关 键 词：S. aureus METHICILLIN-RESISTANT mecA Gene PCR Smoked Fish 

分 类 号：R44[医药卫生—诊断学]