East African Cassava Mosaic Virus and East African Cassava Mosaic Cameroon Virus: Two Species Emerging in Togo  

作　　者：Senya Sakina Allado Kossikouma Djodji Adjata Justin Simon Pita Kodjovi Atassé Dansou-Kodjo Assion Sétu Mivedor Koffi Tozo Senya Sakina Allado;Kossikouma Djodji Adjata;Justin Simon Pita;Kodjovi Atassé Dansou-Kodjo;Assion Sétu Mivedor;Koffi Tozo(Laboratoire de Virologie et de Biotechnologies Vgtales, Ecole Suprieure dAgronomie (ESA), Universit de Lom, Lom, Togo;Laboratory of Plant Virology, Universit Flix Houphout-Boigny, Ple Scientifique et dInnovation, Bingerville, Cte dIvoire;Laboratoire de Physiologie et Biotechnologies Vgtales, Facult des Sciences, Universit de Lom, Lom, Togo)

机构地区：[1]Laboratoire de Virologie et de Biotechnologies Vgtales, Ecole Suprieure dAgronomie (ESA), Universit de Lom, Lom, Togo [2]Laboratory of Plant Virology, Universit Flix Houphout-Boigny, Ple Scientifique et dInnovation, Bingerville, Cte dIvoire [3]Laboratoire de Physiologie et Biotechnologies Vgtales, Facult des Sciences, Universit de Lom, Lom, Togo

出　　处：《Agricultural Sciences》2024年第8期864-876,共13页农业科学（英文）

摘　　要：Cassava mosaic disease (CMD) caused by the whitefly-transmitted Begomoviruses (family Geminiviridae) is a major threat to production of cassava (Manihot esculenta Crantz) in Togo. Survey was conducted in 2020 in the 5 agroecological zones of Togo to assess the status of East African cassava mosaic virus (EACMV), East African cassava mosaic Cameroon virus (EACMCMV) and its distribution. Polymerase chain reaction (PCR) and Sanger sequencing were used for the detection of cassava mosaic Begomoviruses (CMBs) in the sampled leaves. The incidence of EACMV was 47.93% (278/580) and varied between 41.30% (Zone V) and 62.29% (Zone IV) across the agroecological zone but no significant difference was observed. The EACMCMV incidence was 13.67% (38/278) and varied significantly (p ≤ 0.001) through the agroecological zone. Phylogenetic analysis of the viral isolates showed that they are closely related to those from Nigeria, Ghana, and Ivory coast. Nucleotide sequence analysis of CP revealed an overall genetic diversity (π) of around 3.4%. These results showed that EACMV was the predominant virus and that EACMCMV incidence could be more widespread in Togo.Cassava mosaic disease (CMD) caused by the whitefly-transmitted Begomoviruses (family Geminiviridae) is a major threat to production of cassava (Manihot esculenta Crantz) in Togo. Survey was conducted in 2020 in the 5 agroecological zones of Togo to assess the status of East African cassava mosaic virus (EACMV), East African cassava mosaic Cameroon virus (EACMCMV) and its distribution. Polymerase chain reaction (PCR) and Sanger sequencing were used for the detection of cassava mosaic Begomoviruses (CMBs) in the sampled leaves. The incidence of EACMV was 47.93% (278/580) and varied between 41.30% (Zone V) and 62.29% (Zone IV) across the agroecological zone but no significant difference was observed. The EACMCMV incidence was 13.67% (38/278) and varied significantly (p ≤ 0.001) through the agroecological zone. Phylogenetic analysis of the viral isolates showed that they are closely related to those from Nigeria, Ghana, and Ivory coast. Nucleotide sequence analysis of CP revealed an overall genetic diversity (π) of around 3.4%. These results showed that EACMV was the predominant virus and that EACMCMV incidence could be more widespread in Togo.

关 键 词：BEGOMOVIRUS PCR EACMV EACMCMV TOGO Genetic Diversity 

分 类 号：S53[农业科学—作物学]