Occurrence, Molecular Characterization and Phylogenetic Relationship of <i>Aspergillus </i>Species Isolated from Garri Sold in Benue State, North Central, Nigeria  

作　　者：Innocent Okonkwo Ogbonna Innocent Ugochukwu Amai Celestine Uzoma Aguoru Dooshima Charity Amai Innocent Okonkwo Ogbonna;Innocent Ugochukwu Amai;Celestine Uzoma Aguoru;Dooshima Charity Amai(Department of Microbiology, Federal University of Agriculture, Makurdi, Benue State, Nigeria;Department of Botany, Federal University of Agriculture, Makurdi, Benue State, Nigeria;Department of Family Medicine, Federal Medical Centre, Makurdi, Benue State, Nigeria)

机构地区：[1]Department of Microbiology, Federal University of Agriculture, Makurdi, Benue State, Nigeria [2]Department of Botany, Federal University of Agriculture, Makurdi, Benue State, Nigeria [3]Department of Family Medicine, Federal Medical Centre, Makurdi, Benue State, Nigeria

出　　处：《American Journal of Molecular Biology》2021年第4期100-115,共16页美国分子生物学期刊（英文）

摘　　要：<p> Characterization and identification of molds based on cultural and morphological characteristics are often not reliable and frail with limitations. The occurrence of <em>Aspergillus</em> species in garri on sale in markets in Benue State, Nigeria, was studied by molecular techniques. <em>Aspergillus</em> species were isolated and purified on Potato Dextrose Agar. DNA from the purified isolates was extracted using the ZR fungal DNA miniprep and amplified by PCR mix made up of 12.5 μL of Taq 2X Master Mix. Primer sequences for the fungi characterization were internal transcribed spacers ITS 4 and ITS 5. The phylogenetic tree was plotted between the isolated organisms and reference sequences and evolutionary analysis was conducted in MEGA X. Result revealed that one thousand, six hundred and forty-six <em>Aspergilli </em>were isolated comprising of 980 and 666 isolates from the white and yellow garri respectively. <em>Aspergillus</em> <em>flavus, A. fumigatus, A. niger, A. aculeatinus</em>, and <em>A. aculeatus</em> were identified. Twenty percent (20%) of the strains had aflatoxin D structural gene, 50% amplified AFLP and 70% of the strains expressed AFLQ genes needed for the biosynthesis of aflatoxin B1. Majority of the strains that showed the expression of these structural genes were consistent with<em> Aspergillus </em><em>flavus</em>. Phylogenetic tree showed a close relationship among the isolates and their most identical sequence in the NCBI database. </p><p> Characterization and identification of molds based on cultural and morphological characteristics are often not reliable and frail with limitations. The occurrence of <em>Aspergillus</em> species in garri on sale in markets in Benue State, Nigeria, was studied by molecular techniques. <em>Aspergillus</em> species were isolated and purified on Potato Dextrose Agar. DNA from the purified isolates was extracted using the ZR fungal DNA miniprep and amplified by PCR mix made up of 12.5 μL of Taq 2X Master Mix. Primer sequences for the fungi characterization were internal transcribed spacers ITS 4 and ITS 5. The phylogenetic tree was plotted between the isolated organisms and reference sequences and evolutionary analysis was conducted in MEGA X. Result revealed that one thousand, six hundred and forty-six <em>Aspergilli </em>were isolated comprising of 980 and 666 isolates from the white and yellow garri respectively. <em>Aspergillus</em> <em>flavus, A. fumigatus, A. niger, A. aculeatinus</em>, and <em>A. aculeatus</em> were identified. Twenty percent (20%) of the strains had aflatoxin D structural gene, 50% amplified AFLP and 70% of the strains expressed AFLQ genes needed for the biosynthesis of aflatoxin B1. Majority of the strains that showed the expression of these structural genes were consistent with<em> Aspergillus </em><em>flavus</em>. Phylogenetic tree showed a close relationship among the isolates and their most identical sequence in the NCBI database. </p>

关 键 词：Aflatoxin Aspergillus species ASPERGILLOSIS Garri Polymerase Chain Reaction (PCR) Benue State 

分 类 号：Q93[生物学—微生物学]