Morphological, Pathological, Biochemical and Molecular Characterization of <i>Ralstonia solanacearum</i>Isolates in Bangladesh  

作　　者：S. Razia M. S. M. Chowdhury F. M. Aminuzzaman N. Sultana M. Islam S. Razia;M. S. M. Chowdhury;F. M. Aminuzzaman;N. Sultana;M. Islam(Department of Plant Pathology, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh;Plant Pathology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh)

机构地区：[1]Department of Plant Pathology, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh [2]Plant Pathology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh

出　　处：《American Journal of Molecular Biology》2021年第4期142-164,共23页美国分子生物学期刊（英文）

摘　　要：One thirty samples (fifty-five potato tubers, twenty-seven potato stems, three chili stems, twenty-eight soil samples, five weed samples, three banana leaves, and nine water samples) were examined and one hundred six (106) Bangladeshi isolates of <em>Ralstonia solanacearum</em> were isolated and identified. Isolation was made on selective media (Tetrazolium chloride media) and <em>R. solanacearum</em> was identified based on morphological, pathological and biochemical properties and polymerase chain reaction (PCR) by using the species-specific primers. Studies showed that 81.54% (106) samples were positive on tetrazolium chloride solid medium. Among them 90 isolates were virulent and rest of them were avirulent. Fifty isolates were selected for chemical characterization based on hypersensitivity test. <em>R. solanacearum</em> is gram negative, aerobic facultative bacteria on the basis of chemical characterization. Fifty tested isolates expressed as race 3 while in biovar test forty-eight showed as biovar III and the rest two showed as biovar I. In nine tested isolates from the three districts a species-specific band of 280 bp was amplified in PCR that confirmed the identity of <em>R. solanacerum</em>.One thirty samples (fifty-five potato tubers, twenty-seven potato stems, three chili stems, twenty-eight soil samples, five weed samples, three banana leaves, and nine water samples) were examined and one hundred six (106) Bangladeshi isolates of <em>Ralstonia solanacearum</em> were isolated and identified. Isolation was made on selective media (Tetrazolium chloride media) and <em>R. solanacearum</em> was identified based on morphological, pathological and biochemical properties and polymerase chain reaction (PCR) by using the species-specific primers. Studies showed that 81.54% (106) samples were positive on tetrazolium chloride solid medium. Among them 90 isolates were virulent and rest of them were avirulent. Fifty isolates were selected for chemical characterization based on hypersensitivity test. <em>R. solanacearum</em> is gram negative, aerobic facultative bacteria on the basis of chemical characterization. Fifty tested isolates expressed as race 3 while in biovar test forty-eight showed as biovar III and the rest two showed as biovar I. In nine tested isolates from the three districts a species-specific band of 280 bp was amplified in PCR that confirmed the identity of <em>R. solanacerum</em>.

关 键 词：Brown Rot of Potato Latent Infection Ralstonia solanacearum BIOVAR Race 

分 类 号：O62[理学—有机化学]