Investigating Biological Relationships in Burkina Faso Using DNA Testing  

作　　者：Bapio Valérie Elvira Jean Télesphore Bazie Moussa Ouattara Serge Théophile Soubeiga Abdou Azaque Zoure Prosper Bado Abel Sorgho Touwendpoulimdé Isabelle Kiendrebeogo Lassina Traore Soumaïla Sawadogo Luc Zongo Théodora M. Zohoncon Florencia W. Djigma Théophane Albert T. Yonli Mahamoudou Sanou Jacques Simpore Bapio Valérie Elvira Jean Télesphore Bazie;Moussa Ouattara;Serge Théophile Soubeiga;Abdou Azaque Zoure;Prosper Bado;Abel Sorgho;Touwendpoulimdé Isabelle Kiendrebeogo;Lassina Traore;Soumaïla Sawadogo;Luc Zongo;Théodora M. Zohoncon;Florencia W. Djigma;Théophane Albert T. Yonli;Mahamoudou Sanou;Jacques Simpore(Centre de Recherche Biomolculaire Pietro Annigoni (CERBA), Ouagadougou, Burkina Faso;Universit Joseph KI-ZERBO, Unit de Formation en Sciences de la Vie et de la Terre, Laboratoire de Biologie Molculaire et de Gntique (LABIOGENE), Ouagadougou, Burkina Faso;Centre National de la Recherche Scientifique et Technologique (CNRST), Institut de Recherche en Sciences de la Sant (IRSS), Ouagadougou, Burkina Faso;Faculty of Health Sciences, University Saint Thomas dAquin (USTA), Ouagadougou, Burkina Faso;Centre Hospitalier Universitaire Pdiatrie Charles De Gaulle, Universit Joseph Ki-Zerbo, Ouagadougou, Burkina Faso)

机构地区：[1]Centre de Recherche Biomolculaire Pietro Annigoni (CERBA), Ouagadougou, Burkina Faso [2]Universit Joseph KI-ZERBO, Unit de Formation en Sciences de la Vie et de la Terre, Laboratoire de Biologie Molculaire et de Gntique (LABIOGENE), Ouagadougou, Burkina Faso [3]Centre National de la Recherche Scientifique et Technologique (CNRST), Institut de Recherche en Sciences de la Sant (IRSS), Ouagadougou, Burkina Faso [4]Faculty of Health Sciences, University Saint Thomas dAquin (USTA), Ouagadougou, Burkina Faso [5]Centre Hospitalier Universitaire Pdiatrie Charles De Gaulle, Universit Joseph Ki-Zerbo, Ouagadougou, Burkina Faso

出　　处：《American Journal of Molecular Biology》2025年第1期97-109,共13页美国分子生物学期刊(英文)

摘　　要：DNA testing for biological relationships is becoming increasingly common in Burkina Faso. STR analysis remains the most reliable technique for resolving disputes or claims in court regarding biological relationships. This study aimed to establish the links of biological relationships between subjects by analyzing 21 STR loci. The participants were 14 individuals referred to CERBA by the Justice in 2022. Oral or blood samples were taken for each subject. DNA was extracted, and the analysis of DNA polymorphism by PowerPlex® 21 Kit (Part No. DC8902) was performed by capillary electrophoresis on the ABI PRISM 3130 sequencer. DNA profiles were compiled using the GeneMapper IDX software version 1.2. Of the fourteen subjects examined, twelve of these samples had complete genetic profiles, while the other two had partial and absent profiles. The results confirmed the inclusion of three cases of Paternity, one case of maternity, one case of a relationship of brotherhood, and the exclusion of one case of maternity and one case of a relationship of brotherhood. DNA tests improve the resolution of filiations, but they require ethical and cultural awareness and a strengthened legal framework to prevent and protect society.DNA testing for biological relationships is becoming increasingly common in Burkina Faso. STR analysis remains the most reliable technique for resolving disputes or claims in court regarding biological relationships. This study aimed to establish the links of biological relationships between subjects by analyzing 21 STR loci. The participants were 14 individuals referred to CERBA by the Justice in 2022. Oral or blood samples were taken for each subject. DNA was extracted, and the analysis of DNA polymorphism by PowerPlex® 21 Kit (Part No. DC8902) was performed by capillary electrophoresis on the ABI PRISM 3130 sequencer. DNA profiles were compiled using the GeneMapper IDX software version 1.2. Of the fourteen subjects examined, twelve of these samples had complete genetic profiles, while the other two had partial and absent profiles. The results confirmed the inclusion of three cases of Paternity, one case of maternity, one case of a relationship of brotherhood, and the exclusion of one case of maternity and one case of a relationship of brotherhood. DNA tests improve the resolution of filiations, but they require ethical and cultural awareness and a strengthened legal framework to prevent and protect society.

关 键 词：DNA STR IDENTIFICATION Genetic Fingerprint Burkina Faso 

分 类 号：D91[政治法律—法学]