Isolation and Molecular Characterization of Elite Indigenous Rhizobia Nodulating Phaseolus bean (Phaseolus vulgaris L.)  

作　　者：Yusuph Namkeleja Kelvin Mtei Patrick A. Ndakidemi Yusuph Namkeleja;Kelvin Mtei;Patrick A. Ndakidemi(Department of Sustainable Agriculture, Biodiversity and Ecosystem Management, The Nelson Mandela African Institution of Science and Technology, Arusha, Tanzania;Department of Water and Environmental Science and Engineering, The Nelson Mandela African Institution of Science and Technology, Arusha, Tanzania)

机构地区：[1]Department of Sustainable Agriculture, Biodiversity and Ecosystem Management, The Nelson Mandela African Institution of Science and Technology, Arusha, Tanzania [2]Department of Water and Environmental Science and Engineering, The Nelson Mandela African Institution of Science and Technology, Arusha, Tanzania

出　　处：《American Journal of Plant Sciences》2016年第14期1905-1920,共17页美国植物学期刊（英文）

摘　　要：Nowadays application of biological nitrogen fixation (BNF) through rhizobia inoculums is highly promoted as a solution to solve the problem of poor soil fertility in areas where legumes are cultivated. This is due to the fact that, rhizobia enhance nitrogen fixation, induce disease resistance, reduce heavy metal in the soil, facilitate bioavailabity of iron in soil and is environmental friendly. To get rhizobia strains which are suitable for inoculants production, isolation and molecular characterization of elite rhizobia are highly needed. Molecular characterization acts as a spark plug for discovery of many microbes including Rhizobia. Multi Locus Sequence Analysis (MLSA), 16S rRNA gene sequence analysis, DNA-DNA hybridization and SDS-PAGE analysis of the whole-cell proteins are the molecular techniques mostly used in characterizing rhizobia. But before deciding to use or not to use rhizobia inoculants in certain areas, knowing the population size of indigenous rhizobia found in that area is very important, because this is a major factor which determines inoculums responses as well gives clues on which areas need or do not need inoculation. The Most Probable Number (MPN) method is mostly used in enumerating rhizobia population of the soil. Given that, in most of the developing countries, including Tanzania, Biological Nitrogen Fixation (BNF) technology is not fully flourished;more efforts in isolation, molecular characterization of elite rhizobia and estimation of indigenous rhizobia population in various areas are required.Nowadays application of biological nitrogen fixation (BNF) through rhizobia inoculums is highly promoted as a solution to solve the problem of poor soil fertility in areas where legumes are cultivated. This is due to the fact that, rhizobia enhance nitrogen fixation, induce disease resistance, reduce heavy metal in the soil, facilitate bioavailabity of iron in soil and is environmental friendly. To get rhizobia strains which are suitable for inoculants production, isolation and molecular characterization of elite rhizobia are highly needed. Molecular characterization acts as a spark plug for discovery of many microbes including Rhizobia. Multi Locus Sequence Analysis (MLSA), 16S rRNA gene sequence analysis, DNA-DNA hybridization and SDS-PAGE analysis of the whole-cell proteins are the molecular techniques mostly used in characterizing rhizobia. But before deciding to use or not to use rhizobia inoculants in certain areas, knowing the population size of indigenous rhizobia found in that area is very important, because this is a major factor which determines inoculums responses as well gives clues on which areas need or do not need inoculation. The Most Probable Number (MPN) method is mostly used in enumerating rhizobia population of the soil. Given that, in most of the developing countries, including Tanzania, Biological Nitrogen Fixation (BNF) technology is not fully flourished;more efforts in isolation, molecular characterization of elite rhizobia and estimation of indigenous rhizobia population in various areas are required.

关 键 词：Biological Nitrogen Fixation INOCULUMS Rhizobium-Legume Symbiosis Phaseolus Bean 

分 类 号：S15[农业科学—土壤学]