Phytochemical Evaluation and <i>in Vitro</i>Antibacterial Activity of <i>Sphaeranthus indicus</i>(L.)—An Important Antijaundice Medicinal Plant  

作　　者：K. Harathi D. Giribabu C. Varadarajulu Naidu 

机构地区：[1]Department of Biotechnology, Dravidian University, Kuppam, India [2]Department of Biotechnology, Sri Venkateswara University, Tirupati, India

出　　处：《American Journal of Plant Sciences》2017年第5期1011-1021,共11页美国植物学期刊（英文）

摘　　要：An investigation was carried out to study the antibacterial activity of Sphaeranthus indicus from leaf, stem and root extracts by the sequential cold maceration method against selected laboratory bacterial pathogens such as Bacillus subtilis, Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli and Pseudomonas putida by the agar well diffusion method. Zone of inhibition measured (mm) was compared with standard antibiotics such as Tetracycline, Erythromycin and Ampicillin. The organic solvents such as ethanol, methanol, petroleum ether, chloroform as well as distilled water extracts were employed. Among all the extracts, tested ethanolic leaf extracts have showed more antibacterial activity against Klebsiella pneumoniae. Phytochemical screening methods were also done to identify the major secondary metabolites in the present species such as alkaloids, flavonoids, phenols, steroids and tannins. This study concluded that Sphaeranthus indicus had the sufficient antibacterial activity due to the presence of various secondary metabolites.An investigation was carried out to study the antibacterial activity of Sphaeranthus indicus from leaf, stem and root extracts by the sequential cold maceration method against selected laboratory bacterial pathogens such as Bacillus subtilis, Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli and Pseudomonas putida by the agar well diffusion method. Zone of inhibition measured (mm) was compared with standard antibiotics such as Tetracycline, Erythromycin and Ampicillin. The organic solvents such as ethanol, methanol, petroleum ether, chloroform as well as distilled water extracts were employed. Among all the extracts, tested ethanolic leaf extracts have showed more antibacterial activity against Klebsiella pneumoniae. Phytochemical screening methods were also done to identify the major secondary metabolites in the present species such as alkaloids, flavonoids, phenols, steroids and tannins. This study concluded that Sphaeranthus indicus had the sufficient antibacterial activity due to the presence of various secondary metabolites.

关 键 词：Sphaeranthus INDICUS Antibacterial Activity AGAR Well Diffusion PHYTOCHEMICAL Screening 

分 类 号：R28[医药卫生—中药学]