Micropropagation of Carob(Ceratonia siliqua L.)through Adventitious Buds of Immature Embryonic Cotyledons  

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作  者:Brahim El Bouzdoudi Rabah Saidi Zineb Nejjar El Ansari Mohammed L’bachir El Kbiach Patrick Martin Alain Badoc Ahmed Lamarti 

机构地区:[1]Laboratory of Plant Biotechnology, Biology Department, Faculty of Sciences, Abdelmalek Essaadi University, Tetouan, Morocco [2]Department of Matter and Life Sciences, High Normal School, Martil, Morocco [3]Chemistry Department, IUT Béthune, F-62408, Artois University, Béthune, France [4]Axe MIB (Molécules d’Intérêt Biologique), Unité de Recherche Enologie EA 4577, USC 1366 INRA, UFR des Sciences

出  处:《American Journal of Plant Sciences》2017年第9期2180-2195,共16页美国植物学期刊(英文)

摘  要:Adventitious budding from embryonic cotyledons of immature seeds of carob was obtained. The combination of BAP (4.44 μM) and NAA (1.5 μM) furthered the neoformation of adventitious buds. These latter were multiplied on MS medium added with BAP (2.22 μM). Stems and leaves growing were improved by adding 2.02 μM GA3. Elongation was favored by 0.5 μM NAA. 70% of rooting was obtained with 10 μM IBA.Adventitious budding from embryonic cotyledons of immature seeds of carob was obtained. The combination of BAP (4.44 μM) and NAA (1.5 μM) furthered the neoformation of adventitious buds. These latter were multiplied on MS medium added with BAP (2.22 μM). Stems and leaves growing were improved by adding 2.02 μM GA3. Elongation was favored by 0.5 μM NAA. 70% of rooting was obtained with 10 μM IBA.

关 键 词:Ceratonia siliqua L. MICROPROPAGATION Adventitious Bud Embryonic Cotyledons 

分 类 号:S5[农业科学—作物学]

 

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