Proliferation, Multiplication and Improvement of Micro-Propagation System for Mass Clonal Production of Rose through Shoot Tip Culture  

作　　者：Allah Jurio Khaskheli Muhammad Ibrahim Khaskheli Maqsood Ahmad Khaskheli Tahmina Shar Waqas Ahmad Umed Ali Lighari Muhammad Azeem Khaskheli Asad Ali Khaskheli Faisal Hayat Makan 

机构地区：[1]Department of Biotechnology, Sindh Agriculture University, Tando Jam, Pakistan [2]Department of Plant Protection, Sindh Agriculture University, Tando Jam, Pakistan [3]State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, China [4]Collage of Agriculture and Biotechnology, China Agriculture University, Beijing, China [5]Institute of Pomology, Chinese Academy of Agriculture Sciences, Xingcheng, China [6]Department of Animal Nutrition, Sindh Agriculture University, Tando Jam, Pakistan

出　　处：《American Journal of Plant Sciences》2018年第2期296-310,共15页美国植物学期刊（英文）

摘　　要：Present study was conducted to assess the regeneration potential and producing mass-clonal seedlings of Rose through shoot tip culture. A total of 40 explants were cultured on each of basal medium supplemented with different concentrations of BAP, NAA and GA. The observations on the survival rate, days taken to initiate the shoots, total number of shoots and length of shoots, initiation of roots, total number of root, length of roots and number of leaves were investigated. Rose regenerated on MS-Basal medium (control) without addition of growth hormones showed significantly (P < 0.05) lower survival rate and did not show shoots up to the end of experiments. MS-Basal medium supplemented with different concentrations of BAP, NAA and GA showed increasing rate of survival. MS-Basal medium supplemented with highest BAP, NAA and GA concentration (MS-SV) has taken least time to initiate the shoots, whilst supplemented in concentrations of 0.5 and/or 1.0 mg/L, respectively revealed more time. Rooted plants were transplanted into the substrate and acclimatized in the laboratory greenhouse (humid cavity). The acclimatization in the humid cavity showed optimistic effect on the number of survived plants.Present study was conducted to assess the regeneration potential and producing mass-clonal seedlings of Rose through shoot tip culture. A total of 40 explants were cultured on each of basal medium supplemented with different concentrations of BAP, NAA and GA. The observations on the survival rate, days taken to initiate the shoots, total number of shoots and length of shoots, initiation of roots, total number of root, length of roots and number of leaves were investigated. Rose regenerated on MS-Basal medium (control) without addition of growth hormones showed significantly (P < 0.05) lower survival rate and did not show shoots up to the end of experiments. MS-Basal medium supplemented with different concentrations of BAP, NAA and GA showed increasing rate of survival. MS-Basal medium supplemented with highest BAP, NAA and GA concentration (MS-SV) has taken least time to initiate the shoots, whilst supplemented in concentrations of 0.5 and/or 1.0 mg/L, respectively revealed more time. Rooted plants were transplanted into the substrate and acclimatized in the laboratory greenhouse (humid cavity). The acclimatization in the humid cavity showed optimistic effect on the number of survived plants.

关 键 词：ROSE Tissue Culture EXPLANTS BAP NAA and GA 

分 类 号：R73[医药卫生—肿瘤]