Micropropagation of the Moroccan Endemic Plant <i>Thymus broussonetii</i>Boiss. with Aromatic-Medicinal Value and Conservation Concern  

作　　者：Zineb Nejjar El Ansari Ibtissam Boussaoudi Rajae Benkaddour Houda Tahiri Aïcha El Oualkadi Alain Badoc Patrick Martin Ahmed Lamarti Zineb Nejjar El Ansari;Ibtissam Boussaoudi;Rajae Benkaddour;Houda Tahiri;Aïcha El Oualkadi;Alain Badoc;Patrick Martin;Ahmed Lamarti(Laboratory of Plant Biotechnology, Biology Department, Faculty of Sciences, Abdelmalek Essaadi University, Tetouan, Morocco;Genetic Improvement of Plants Laboratory/National Institute of Agronomic Research (INRA), Tangier, Morocco;Axe MIB (Molécules d’Intérêt Biologique), Unité de Recherche Œnologie, UFR des Sciences Pharmaceutiques, USC INRAE 1366-EA 4577, ISVV (Institut des Sciences de la Vigne et du Vin), Villenave-d’Ornon, France;Université d’Artois, UniLaSalle, ULR7519-Unité Transformations & Agroressources, Béthune, France)

机构地区：[1]Laboratory of Plant Biotechnology, Biology Department, Faculty of Sciences, Abdelmalek Essaadi University, Tetouan, Morocco [2]Genetic Improvement of Plants Laboratory/National Institute of Agronomic Research (INRA), Tangier, Morocco [3]Axe MIB (Molécules d’Intérêt Biologique), Unité de Recherche Œ nologie, UFR des Sciences Pharmaceutiques, USC INRAE 1366-EA 4577, ISVV (Institut des Sciences de la Vigne et du Vin), Villenave-d’Ornon, France [4]Université d’Artois, UniLaSalle, ULR7519-Unité Transformations & Agroressources, Béthune, France

出　　处：《American Journal of Plant Sciences》2020年第6期913-938,共25页美国植物学期刊（英文）

摘　　要：Micropropagation from shoot tips and nodal segments was carried out for the conservation and domestication of spontaneous Moroccan thyme, <i><span style="font-family:Verdana;">Thymus broussonetii</span></i><i> </i><span style="font-family:Verdana;">Boiss. subsp. </span><i><span style="font-family:Verdana;">broussonetii</span></i><span style="font-family:Verdana;"> (endemic threatened). The mineral composition of the culture medium, as well as the succession of different growth regulators, influenced the </span><i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"> growth of this species. Sterilized achenes of </span><i><span style="font-family:Verdana;">T. broussonetii</span></i><i> </i><span style="font-family:Verdana;">were able to germinate on an agar medium containing Gautheret macronutrients with a rate of 25% and a degree of contamination of less than 4%. Shoot apices of 15-day seedlings (two cotyledon leaves) were cultivated on SD + 0.46 μM Kin medium and the explants obtained were transplanted every month. Six macronutrients (MS, B</span>_{<span style="font-family:Verdana;">5</span>}<span style="font-family:Verdana;">, SH, SD, MS</span>_{<span style="font-family:Verdana;">m</span>} <span><span style="font-family:Verdana;">and N</span>_{<span style="font-family:Verdana;">30</span>}<span style="font-family:Verdana;">K) were tested and N</span>_{<span style="font-family:Verdana;">30</span>}<span style="font-family:Verdana;">K was chosen for the following experiments. Seven cytokinins (Kin, BAP, 2iP, DPU, adenine, Zeat and TDZ) at 0.46, 0.93 and 2.32 μM/l were evaluated and the addition of 0.93 μM adenine to N</span>_{<span style="font-family:Verdana;">30</span>}<span style="font-family:Verdana;">K medium favored significantly the induction of buds and the elongation of explants. Three polyamines (putrescine, spermidine and spermine) at 2, 5, 10 and 20 μM/l were tested. A better multiplication of buds, shoots and roots was notedMicropropagation from shoot tips and nodal segments was carried out for the conservation and domestication of spontaneous Moroccan thyme, <i><span style="font-family:Verdana;">Thymus broussonetii</span></i><i> </i><span style="font-family:Verdana;">Boiss. subsp. </span><i><span style="font-family:Verdana;">broussonetii</span></i><span style="font-family:Verdana;"> (endemic threatened). The mineral composition of the culture medium, as well as the succession of different growth regulators, influenced the </span><i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"> growth of this species. Sterilized achenes of </span><i><span style="font-family:Verdana;">T. broussonetii</span></i><i> </i><span style="font-family:Verdana;">were able to germinate on an agar medium containing Gautheret macronutrients with a rate of 25% and a degree of contamination of less than 4%. Shoot apices of 15-day seedlings (two cotyledon leaves) were cultivated on SD + 0.46 μM Kin medium and the explants obtained were transplanted every month. Six macronutrients (MS, B</span>_{<span style="font-family:Verdana;">5</span>}<span style="font-family:Verdana;">, SH, SD, MS</span>_{<span style="font-family:Verdana;">m</span>} <span><span style="font-family:Verdana;">and N</span>_{<span style="font-family:Verdana;">30</span>}<span style="font-family:Verdana;">K) were tested and N</span>_{<span style="font-family:Verdana;">30</span>}<span style="font-family:Verdana;">K was chosen for the following experiments. Seven cytokinins (Kin, BAP, 2iP, DPU, adenine, Zeat and TDZ) at 0.46, 0.93 and 2.32 μM/l were evaluated and the addition of 0.93 μM adenine to N</span>_{<span style="font-family:Verdana;">30</span>}<span style="font-family:Verdana;">K medium favored significantly the induction of buds and the elongation of explants. Three polyamines (putrescine, spermidine and spermine) at 2, 5, 10 and 20 μM/l were tested. A better multiplication of buds, shoots and roots was noted

关 键 词：Thymus broussonetii MICROPROPAGATION Shoot Tip Nodes Macronutrients Cytokinins Polyamines AUXINS Prototypes CONSERVATION 

分 类 号：O17[理学—数学]