Drought Tolerance Enhancement with Co-Overexpression of DREB2A and APX in Indica Rice (<i>Oryza sativa</i>L.)  

作　　者：Jagtap Sandhya Talakayala Ashwini Ravula Manisha Mahankali Vinodha Ankanagari Srinivas Jagtap Sandhya;Talakayala Ashwini;Ravula Manisha;Mahankali Vinodha;Ankanagari Srinivas(Department of Genetics & Biotechnology, Osmania University, Hyderabad, India)

机构地区：[1]Department of Genetics & Biotechnology, Osmania University, Hyderabad, India

出　　处：《American Journal of Plant Sciences》2021年第2期234-258,共25页美国植物学期刊（英文）

摘　　要：Rice<b> </b><span style="font-family:Verdana;">is a major cereal crop providing food and energy to more than half of world’s population and drought is a challenging abiotic stress limiting rice production</span><span style="font-family:Verdana;">.</span><span style="font-family:Verdana;"> Engineering drought tolerance trait is a major bottle neck because of multigenic control and complex nature. Two promising candidate genes utilized in engineering drought tolerance include DREB2A transcription factor (a master regulator of downstream stress inducible genes) and APX (an important ROS scavenging enzyme). Overexpression of DREB genes ha</span><span style="font-family:Verdana;">s</span><span style="font-family:Verdana;"> shown encouraging results but with </span><span style="font-family:Verdana;">a </span><span style="font-family:Verdana;">negative impact on plant morphology and production. Moreover, co-expression of DREB2A and APX genes</span><span style="font-family:Verdana;">’</span><span style="font-family:Verdana;"> influence on drought stress has not been studied. Hence, in the present study</span><span style="font-family:Verdana;">,</span><span style="font-family:;" "=""><span style="font-family:Verdana;"> overexpression of single genes DREB2A or APX and co-expression of these genes were studied for enhancement of drought tolerance in indica rice. Both genes under control of CaMV 35S promoter were transferred by </span><i><span style="font-family:Verdana;">Agrobacterium</span></i><span style="font-family:Verdana;"> transformation into rice variety BPT5204 popular for slender grains in South India. Confirmation of T-DNA integration into rice genome was done with PCR analysis of transgenes. Homozygous transgenic lines of DREB2A, </span><span><span style="font-family:Verdana;">APX and DREB2A-APX generated in T</span>_{<span style="font-family:Verdana;">3</span>}<span style="font-family:Verdana;"> generation were evaluated for</span></span><span style="font-family:Verdana;"> drought tolerance during sRice<b> </b><span style="font-family:Verdana;">is a major cereal crop providing food and energy to more than half of world’s population and drought is a challenging abiotic stress limiting rice production</span><span style="font-family:Verdana;">.</span><span style="font-family:Verdana;"> Engineering drought tolerance trait is a major bottle neck because of multigenic control and complex nature. Two promising candidate genes utilized in engineering drought tolerance include DREB2A transcription factor (a master regulator of downstream stress inducible genes) and APX (an important ROS scavenging enzyme). Overexpression of DREB genes ha</span><span style="font-family:Verdana;">s</span><span style="font-family:Verdana;"> shown encouraging results but with </span><span style="font-family:Verdana;">a </span><span style="font-family:Verdana;">negative impact on plant morphology and production. Moreover, co-expression of DREB2A and APX genes</span><span style="font-family:Verdana;">’</span><span style="font-family:Verdana;"> influence on drought stress has not been studied. Hence, in the present study</span><span style="font-family:Verdana;">,</span><span style="font-family:;" "=""><span style="font-family:Verdana;"> overexpression of single genes DREB2A or APX and co-expression of these genes were studied for enhancement of drought tolerance in indica rice. Both genes under control of CaMV 35S promoter were transferred by </span><i><span style="font-family:Verdana;">Agrobacterium</span></i><span style="font-family:Verdana;"> transformation into rice variety BPT5204 popular for slender grains in South India. Confirmation of T-DNA integration into rice genome was done with PCR analysis of transgenes. Homozygous transgenic lines of DREB2A, </span><span><span style="font-family:Verdana;">APX and DREB2A-APX generated in T</span>_{<span style="font-family:Verdana;">3</span>}<span style="font-family:Verdana;"> generation were evaluated for</span></span><span style="font-family:Verdana;"> drought tolerance during s

关 键 词：APX DREB2A Reactive Oxygen Species Transgenic Rice Drought Tolerance 

分 类 号：S51[农业科学—作物学]