Influence of Amino Acids on the Secondary Somatic Embryogenesis Proliferation Process of Moroccan Cork Oak (<i>Quercus suber</i>L.)  

作　　者：Naouar Ben Ali Lamarti Ahmad Naouar Ben Ali;Lamarti Ahmad(Plant Biotechnology Laboratory, Department of Biology, Faculty of Sciences of Tetouan, Abdelmalek Essaadi University, Tetouan, Morocco)

机构地区：[1]Plant Biotechnology Laboratory, Department of Biology, Faculty of Sciences of Tetouan, Abdelmalek Essaadi University, Tetouan, Morocco

出　　处：《American Journal of Plant Sciences》2021年第4期603-613,共11页美国植物学期刊（英文）

摘　　要：The present study aims to explore the regeneration potential of Moroccan cork oak through the secondary somatic embryogenesis process. Particularly, we focus on the analysis of amino acids influence on the quantity and quality </span><span style="font-family:Verdana;">of the regenerated secondary embryos. The amino acids tested are: Gluta</span><span style="font-family:Verdana;">mine, </span><span style="font-family:Verdana;">asparagine, arginine, tryptophane, methionine, casein hydrolysate and urea.</span><span style="font-family:Verdana;"> Each amino acid is added in the Margara (N30K) medium at different concentra</span><span style="font-family:Verdana;">tions ranged between 10 and 500 mg/l. The results are collected after 2</span><span style="font-family:Verdana;"> months of culture. First analysis shows that the glutamine and the casein hydrolysate </span><span style="font-family:Verdana;">gives a maximum number of somatic embryos, clusters and pre-embryos</span><span style="font-family:Verdana;"> newly formed on the clusters. By comparison to the control medium, the increase of </span><span style="font-family:Verdana;">the secondary embryos number directly formed exceed 36% in the case of</span><span style="font-family:Verdana;"> casein hydrolysate and 35% of in the case of glutamine both at the concentration of 30 mg/l. However, the test of the combination of these amino acids did not have any significant results. In terms of quality, the influence of amino acids on the morphology of secondary embryos was analyzed.The present study aims to explore the regeneration potential of Moroccan cork oak through the secondary somatic embryogenesis process. Particularly, we focus on the analysis of amino acids influence on the quantity and quality </span><span style="font-family:Verdana;">of the regenerated secondary embryos. The amino acids tested are: Gluta</span><span style="font-family:Verdana;">mine, </span><span style="font-family:Verdana;">asparagine, arginine, tryptophane, methionine, casein hydrolysate and urea.</span><span style="font-family:Verdana;"> Each amino acid is added in the Margara (N30K) medium at different concentra</span><span style="font-family:Verdana;">tions ranged between 10 and 500 mg/l. The results are collected after 2</span><span style="font-family:Verdana;"> months of culture. First analysis shows that the glutamine and the casein hydrolysate </span><span style="font-family:Verdana;">gives a maximum number of somatic embryos, clusters and pre-embryos</span><span style="font-family:Verdana;"> newly formed on the clusters. By comparison to the control medium, the increase of </span><span style="font-family:Verdana;">the secondary embryos number directly formed exceed 36% in the case of</span><span style="font-family:Verdana;"> casein hydrolysate and 35% of in the case of glutamine both at the concentration of 30 mg/l. However, the test of the combination of these amino acids did not have any significant results. In terms of quality, the influence of amino acids on the morphology of secondary embryos was analyzed.

关 键 词：Quercus suber L. Secondary Embryos Amino Acids Glutamine ASPARAGINE Casein Hydrolysat 

分 类 号：O62[理学—有机化学]