Genetic Variation among Seedling of Pumpkins Genotypes through SDS-Page  

作　　者：Muhammad Ikram Niaz Ali Muhammad Ishaq Muhammad Romman Yasir Islam Naeem Khan Muhammad Ikram;Niaz Ali;Muhammad Ishaq;Muhammad Romman;Yasir Islam;Naeem Khan(Department of Botany, Hazara University Mansehra, Khyber Pakhtunkhwa, Pakistan;Department of Botany, University of Chitral, Khyber Pakhtunkhwa, Pakistan;Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan)

机构地区：[1]Department of Botany, Hazara University Mansehra, Khyber Pakhtunkhwa, Pakistan [2]Department of Botany, University of Chitral, Khyber Pakhtunkhwa, Pakistan [3]Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan

出　　处：《American Journal of Plant Sciences》2021年第5期711-719,共9页美国植物学期刊（英文）

摘　　要：Genetic diversity in 30 genotypes of Pumpkins <span style="font-family:Verdana;">was</span><span style="font-family:Verdana;"> collected from unexplored mountainous areas of Khyber Pakhtunkhwa, Pakistan was investigated through biochemical characterization. For biochemical characterization, Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis was carried out. The seed proteins were resolved on 7.5% and 15% polyacrylamide gel. A total of 35% genetic disagreement was observed in the collected lines with linkage distances ranging from 0.00</span><span style="font-family:Verdana;"> - </span><span style="font-family:Verdana;">0.83 (percent disagreement). Similarly, cluster analysis sorted total germplasm on the basis of 12 bands (total bands) into eight clusters. Present study revealed a considerable amount of genetic diversity explored in pumpkin germplasm, Cluster analysis exhibited moderate level of genetic diversity;to broaden the gene pool. Further collection of the important germplasm is needed </span><span style="font-family:Verdana;">to be </span><span style="font-family:Verdana;">used in the development of improved cultivars with respect to quality and quantity.</span>Genetic diversity in 30 genotypes of Pumpkins <span style="font-family:Verdana;">was</span><span style="font-family:Verdana;"> collected from unexplored mountainous areas of Khyber Pakhtunkhwa, Pakistan was investigated through biochemical characterization. For biochemical characterization, Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis was carried out. The seed proteins were resolved on 7.5% and 15% polyacrylamide gel. A total of 35% genetic disagreement was observed in the collected lines with linkage distances ranging from 0.00</span><span style="font-family:Verdana;"> - </span><span style="font-family:Verdana;">0.83 (percent disagreement). Similarly, cluster analysis sorted total germplasm on the basis of 12 bands (total bands) into eight clusters. Present study revealed a considerable amount of genetic diversity explored in pumpkin germplasm, Cluster analysis exhibited moderate level of genetic diversity;to broaden the gene pool. Further collection of the important germplasm is needed </span><span style="font-family:Verdana;">to be </span><span style="font-family:Verdana;">used in the development of improved cultivars with respect to quality and quantity.</span>

关 键 词：Genetic Diversity SDS-PAGE PUMPKIN Cluster Analysis 

分 类 号：S51[农业科学—作物学]