Heterogeneous Expression and Purification of the Wheat VRN1 K-Box Domain Suggest the Formation of a Tetramer of the VRN1 Protein  

作　　者：Takafumi Itoh Ayane Kamiya Moe Kimura Koji Murai Takafumi Itoh;Ayane Kamiya;Moe Kimura;Koji Murai(Faculty of Bioscience and Biotechnology, Fukui Prefectural University, Fukui, Japan;Department of Sustainable Agri-Culture, Fukui Prefectural University, Fukui, Japan)

机构地区：[1]Faculty of Bioscience and Biotechnology, Fukui Prefectural University, Fukui, Japan [2]Department of Sustainable Agri-Culture, Fukui Prefectural University, Fukui, Japan

出　　处：《American Journal of Plant Sciences》2021年第7期1002-1010,共9页美国植物学期刊（英文）

摘　　要：In cereal species such as wheat (</span><i><span style="font-family:Verdana;">Ttiticum aestivum</span></i><span style="font-family:Verdana;">) and barley (</span><i><span style="font-family:Verdana;">Hordeum vulg</span><span style="font-family:Verdana;">are</span></i><span><span style="font-family:Verdana;">), many studies have indicated that</span><i><span style="font-family:Verdana;"> VERNALIZATION </span></i><span style="font-family:Verdana;">1</span><i> </i><span style="font-family:Verdana;">(</span><i><span style="font-family:Verdana;">VRN</span></i><span style="font-family:Verdana;">1)</span></span><span style="font-family:Verdana;"> functions as a flowering promoter, which activates florigen gene expression. The wheat florigen gene </span><i><span style="font-family:Verdana;">Wheat FLOWERING LOCUS T</span></i><span style="font-family:Verdana;"> (</span><i><span style="font-family:Verdana;">WFT</span></i><span style="font-family:Verdana;">, which is identical to </span><i><span style="font-family:Verdana;">VRN</span></i><span style="font-family:Verdana;">3) is an integrator of the vernalization, photoperiod and au</span><span><span style="font-family:Verdana;">tonomous pathways in wheat flowering, and the </span><i><span style="font-family:Verdana;">WFT</span></i><span style="font-family:Verdana;"> expression is corre</span></span><span style="font-family:Verdana;">lated with the </span><i><span style="font-family:Verdana;">V</span><span style="font-family:Verdana;">RN</span></i><span><span style="font-family:Verdana;">1</span><i> </i><span style="font-family:Verdana;">expression. </span><i><span style="font-family:Verdana;">VRN</span></i><span style="font-family:Verdana;">1</span><i> </i><span style="font-family:Verdana;">encodes an APETALA1/FRUITFULL-lik</span></span><span style="font-family:Verdana;">e MADS-box transcription factor which expression is induced by vernalization, leading to flowering thorough up-regulation of </span><i><span style="font-family:Verdana;">WFT</span></i><span style="font-familIn cereal species such as wheat (</span><i><span style="font-family:Verdana;">Ttiticum aestivum</span></i><span style="font-family:Verdana;">) and barley (</span><i><span style="font-family:Verdana;">Hordeum vulg</span><span style="font-family:Verdana;">are</span></i><span><span style="font-family:Verdana;">), many studies have indicated that</span><i><span style="font-family:Verdana;"> VERNALIZATION </span></i><span style="font-family:Verdana;">1</span><i> </i><span style="font-family:Verdana;">(</span><i><span style="font-family:Verdana;">VRN</span></i><span style="font-family:Verdana;">1)</span></span><span style="font-family:Verdana;"> functions as a flowering promoter, which activates florigen gene expression. The wheat florigen gene </span><i><span style="font-family:Verdana;">Wheat FLOWERING LOCUS T</span></i><span style="font-family:Verdana;"> (</span><i><span style="font-family:Verdana;">WFT</span></i><span style="font-family:Verdana;">, which is identical to </span><i><span style="font-family:Verdana;">VRN</span></i><span style="font-family:Verdana;">3) is an integrator of the vernalization, photoperiod and au</span><span><span style="font-family:Verdana;">tonomous pathways in wheat flowering, and the </span><i><span style="font-family:Verdana;">WFT</span></i><span style="font-family:Verdana;"> expression is corre</span></span><span style="font-family:Verdana;">lated with the </span><i><span style="font-family:Verdana;">V</span><span style="font-family:Verdana;">RN</span></i><span><span style="font-family:Verdana;">1</span><i> </i><span style="font-family:Verdana;">expression. </span><i><span style="font-family:Verdana;">VRN</span></i><span style="font-family:Verdana;">1</span><i> </i><span style="font-family:Verdana;">encodes an APETALA1/FRUITFULL-lik</span></span><span style="font-family:Verdana;">e MADS-box transcription factor which expression is induced by vernalization, leading to flowering thorough up-regulation of </span><i><span style="font-family:Verdana;">WFT</span></i><span style="font-famil

关 键 词：Flowering MADS-Box Protein VRN1 Wheat (Triticum aestivum) 

分 类 号：S51[农业科学—作物学]