<i>In Vitro</i>Propagation and Cytological Analysis of <i>Boerhaavia diffusa </i>L—An Important Medicinal Plant Species of Bangladesh  

作　　者：Sweety Majumder Animesh Biswas Mohammad Mahbubur Rahman Sweety Majumder;Animesh Biswas;Mohammad Mahbubur Rahman(Department of Botany, University of Chittagong, Chittagong, Bangladesh)

机构地区：[1]Department of Botany, University of Chittagong, Chittagong, Bangladesh

出　　处：《American Journal of Plant Sciences》2021年第9期1347-1360,共14页美国植物学期刊（英文）

摘　　要：An effective and consistent <i>in vitro</i> propagation technique for <i>Boerhaavia diffusa</i><b> </b>L.<b> </b>was developed using different types of explants and media compositions. Shoot apex and nodal explants of field grown plants were aseptically cultured on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of auxins (IAA and NAA) and cytokinins (BAP and Kn). The maximum number of multiple shoot buds (6.65 ± 0.09) obtain</span></span><span><span><span style="font-family:"">ed</span></span></span><span><span><span style="font-family:""> from nodal explants on MS medium containing 2.0 mg/l BAP + 0.5 mg/l IAA. Multiple shoot buds underwent rapid elongation (4.24 ± 0.06 cm) on MS media fortified with 3.0 mg/l BAP + 0.5 mg/l NAA. Half strength MS media supplemented with 1.0 mg/l IBA + 0.5 mg/l IAA was better for induction and proliferation (6.42 ± 0.07) of roots and 75% of plantlets were successfully acclimatized to <i>ex vitro</i> condition, exhibiting a normal development. Somatic chromosome number of <i>in vivo</i> and <i>in vitro</i> grown plant</span></span></span><span><span><span style="font-family:"">s</span></span></span><span><span><span style="font-family:""> w</span></span></span><span><span><span style="font-family:"">ere</span></span></span><span><span><span style="font-family:""> confirmed to be 2n = 26. Chromosome length ranged from 1.40 to 2.43 μm in the mother plants and 1.34 to 2.31 μm for <i>in vitro</i> grown plant</span></span></span><span><span><span style="font-family:"">s</span></span></span><span><span><span style="font-family:"">. The total form percent (TF%) of mother and <i>in vitro</i> grown plant</span></span></span><span><span><span style="font-family:"">s</span></span></span><span><span><span style="font-family:""> was 42.91% and 41.16%, respectively. The karyotype formula of <i>in vivo</i> grown plants was 2n = 26 = 4L^sm + 6M^sm + 2M^m + 14S^m, whereas that of the <i>in vitro</i> grown plantAn effective and consistent <i>in vitro</i> propagation technique for <i>Boerhaavia diffusa</i><b> </b>L.<b> </b>was developed using different types of explants and media compositions. Shoot apex and nodal explants of field grown plants were aseptically cultured on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of auxins (IAA and NAA) and cytokinins (BAP and Kn). The maximum number of multiple shoot buds (6.65 ± 0.09) obtain</span></span><span><span><span style="font-family:"">ed</span></span></span><span><span><span style="font-family:""> from nodal explants on MS medium containing 2.0 mg/l BAP + 0.5 mg/l IAA. Multiple shoot buds underwent rapid elongation (4.24 ± 0.06 cm) on MS media fortified with 3.0 mg/l BAP + 0.5 mg/l NAA. Half strength MS media supplemented with 1.0 mg/l IBA + 0.5 mg/l IAA was better for induction and proliferation (6.42 ± 0.07) of roots and 75% of plantlets were successfully acclimatized to <i>ex vitro</i> condition, exhibiting a normal development. Somatic chromosome number of <i>in vivo</i> and <i>in vitro</i> grown plant</span></span></span><span><span><span style="font-family:"">s</span></span></span><span><span><span style="font-family:""> w</span></span></span><span><span><span style="font-family:"">ere</span></span></span><span><span><span style="font-family:""> confirmed to be 2n = 26. Chromosome length ranged from 1.40 to 2.43 μm in the mother plants and 1.34 to 2.31 μm for <i>in vitro</i> grown plant</span></span></span><span><span><span style="font-family:"">s</span></span></span><span><span><span style="font-family:"">. The total form percent (TF%) of mother and <i>in vitro</i> grown plant</span></span></span><span><span><span style="font-family:"">s</span></span></span><span><span><span style="font-family:""> was 42.91% and 41.16%, respectively. The karyotype formula of <i>in vivo</i> grown plants was 2n = 26 = 4L^sm + 6M^sm + 2M^m + 14S^m, whereas that of the <i>in vitro</i> grown plant

关 键 词：Boerhaavia diffusa EXPLANT MICROPROPAGATION Chromosome KARYOTYPE 

分 类 号：O17[理学—数学]