Strong Allelopathic Activities of Leaves and Cultured Cells of <i>Spiraea thunbergii</i>Assayed by the Protoplast Co-Culture Method with Digital Image Analysis: Evaluation of <i>Cis</i>- and <i>Trans</i>-Cinnamic Acid as Allelochemicals  

作　　者：Sakae Suzuki Mari Kimura Rei Yasuda Naoya Wasano Yutaka Sasamoto Yoshiharu Fujii Hamako Sasamoto Sakae Suzuki;Mari Kimura;Rei Yasuda;Naoya Wasano;Yutaka Sasamoto;Yoshiharu Fujii;Hamako Sasamoto(Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Japan;Institute of Biological Control, Faculty of Agriculture, Kyusyu University, Fukuoka, Japan)

机构地区：[1]Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Japan [2]Institute of Biological Control, Faculty of Agriculture, Kyusyu University, Fukuoka, Japan

出　　处：《American Journal of Plant Sciences》2021年第11期1673-1690,共18页美国植物学期刊（英文）

摘　　要：The inhibitory allelopathic activities of leaves and leaf-origin suspension cultured cells of <i>Spiraea</i> <i>thunbergii</i>, and putative allelochemicals, <i>cis</i>- and <i>trans</i>-cinnamic acid, were investigated by the protoplast co-culture method with digital image analysis (DIA-PP method) using lettuce as a recipient. The optimal conditions of 2,4-dichlorophenoxyacetic acid (2,4-D) and benzyladenine (BA) for the cell division of <i>S</i>. <i>thunbergii</i> protoplasts were first examined using 50 μL liquid MS basal medium containing 3% sucrose, and 0.8 M mannitol in a 96-well culture plate. The hormonal condition for co-culture, 1 μM 2,4-D plus 0.1 μM BA, which was optimal for lettuce protoplast growth, was sub-optimal for <i>S</i>. <i>thunbergii</i> protoplasts. Effects of co-culture on the three stages of lettuce protoplast growth, <i>i.e.</i>, cell wall formation, cell division, and yellow pigment accumulation, were examined. Protoplasts of leaf and suspension cells of <i>S</i>. <i>thunbergii</i> strongly inhibited lettuce protoplast growth at the cell division stage (100% inhibition at 80 - 100 × 10³ mL^-1), but not so much at the other two stages. Both <i>cis</i>- and <i>trans</i>-cinnamic acid, showed the strongest inhibition at the cell wall formation stage, and 100% inhibition at the cell division stage at 100 μM. These results were compared with those obtained in a lettuce seedling growth test, using different allelopathic plants, and their allelochemicals were studied by the DIA-PP method.The inhibitory allelopathic activities of leaves and leaf-origin suspension cultured cells of <i>Spiraea</i> <i>thunbergii</i>, and putative allelochemicals, <i>cis</i>- and <i>trans</i>-cinnamic acid, were investigated by the protoplast co-culture method with digital image analysis (DIA-PP method) using lettuce as a recipient. The optimal conditions of 2,4-dichlorophenoxyacetic acid (2,4-D) and benzyladenine (BA) for the cell division of <i>S</i>. <i>thunbergii</i> protoplasts were first examined using 50 μL liquid MS basal medium containing 3% sucrose, and 0.8 M mannitol in a 96-well culture plate. The hormonal condition for co-culture, 1 μM 2,4-D plus 0.1 μM BA, which was optimal for lettuce protoplast growth, was sub-optimal for <i>S</i>. <i>thunbergii</i> protoplasts. Effects of co-culture on the three stages of lettuce protoplast growth, <i>i.e.</i>, cell wall formation, cell division, and yellow pigment accumulation, were examined. Protoplasts of leaf and suspension cells of <i>S</i>. <i>thunbergii</i> strongly inhibited lettuce protoplast growth at the cell division stage (100% inhibition at 80 - 100 × 10³ mL^-1), but not so much at the other two stages. Both <i>cis</i>- and <i>trans</i>-cinnamic acid, showed the strongest inhibition at the cell wall formation stage, and 100% inhibition at the cell division stage at 100 μM. These results were compared with those obtained in a lettuce seedling growth test, using different allelopathic plants, and their allelochemicals were studied by the DIA-PP method.

关 键 词：ALLELOPATHY Cinnamic Acid Protoplast Co-Culture Spiraea thunbergii 

分 类 号：Q94[生物学—植物学]