Molecular Assessment of Athecate Dinoflagellates of the Order Gymnodiniales (Dinophyceae) in Todos Santos Bay, Baja California México  

作　　者：Alexis De Jesús Escarcega-Bata Mary Carmen Ruiz-de la Torre María Luisa Nuñez Resendiz Luis Manuel Enriquez-Paredes Kurt M. Dreckmann Abel Sentíes Alexis De Jesús Escarcega-Bata;Mary Carmen Ruiz-de la Torre;María Luisa Nuñez Resendiz;Luis Manuel Enriquez-Paredes;Kurt M. Dreckmann;Abel Sentíes(Marine Science Faculty, Autonomous University of Baja California, Ensenada, México;Department of Hydrobiology, Universidad Autónoma Metropolitana-Iztapalapa, México, México)

机构地区：[1]Marine Science Faculty, Autonomous University of Baja California, Ensenada, México [2]Department of Hydrobiology, Universidad Autónoma Metropolitana-Iztapalapa, México, México

出　　处：《American Journal of Plant Sciences》2021年第12期1926-1944,共19页美国植物学期刊（英文）

摘　　要：The </span><span style="font-family:"">Gymnodiniales </span><span style="font-family:"">are</span><span style="font-family:""> the most important group of athecate dinoflagellates both for its abundance and distribution and for the harmful potential of several of its species. Although morphologically it is well known, phylogenetically it has been very little studied. HABs impact important coastal activities in Todos Santos Bay, so a detailed characterization of potentially toxic phytoplankton species is crucial in monitoring surveys. Therefore, the aim of the present <span>study was to carry out a molecular assessment to the morphospecies of Gymnodiniales currently recorded in Todos Santos Bay, from which discussed their phylogenetic relationships.</span></span><span style="font-family:""> </span><span style="font-family:"">120 samples were obtained from monthly</span><span style="font-family:""> samplings during 2019 to 2020. For all of them</span><span style="font-family:"">,</span><span style="font-family:""> 18 s small subunit (SSU) and 28 s large subunit (LSU) genes of rDNA were amplified and sequenced using single-cell PCR. Also, they were photographed. A phylogenetic analysis was performed on MrBayes from the combined 18 s - 28 s data set. The presence of 15 phylogenetic entities w</span><span style="font-family:"">as</span><span style="font-family:""> confirmed, four of which constituted new records for the Mexican Pacific, as well as three harmful species, one toxic <span>and two bloom-forming. Our molecular results evidenced the need to re-evaluate</span> the current taxonomic system of athecate dinoflagellates at all hierarchical levels, integrating them with morphological evidence without preponderance of this criterion over taxonomic decisions.The </span><span style="font-family:"">Gymnodiniales </span><span style="font-family:"">are</span><span style="font-family:""> the most important group of athecate dinoflagellates both for its abundance and distribution and for the harmful potential of several of its species. Although morphologically it is well known, phylogenetically it has been very little studied. HABs impact important coastal activities in Todos Santos Bay, so a detailed characterization of potentially toxic phytoplankton species is crucial in monitoring surveys. Therefore, the aim of the present <span>study was to carry out a molecular assessment to the morphospecies of Gymnodiniales currently recorded in Todos Santos Bay, from which discussed their phylogenetic relationships.</span></span><span style="font-family:""> </span><span style="font-family:"">120 samples were obtained from monthly</span><span style="font-family:""> samplings during 2019 to 2020. For all of them</span><span style="font-family:"">,</span><span style="font-family:""> 18 s small subunit (SSU) and 28 s large subunit (LSU) genes of rDNA were amplified and sequenced using single-cell PCR. Also, they were photographed. A phylogenetic analysis was performed on MrBayes from the combined 18 s - 28 s data set. The presence of 15 phylogenetic entities w</span><span style="font-family:"">as</span><span style="font-family:""> confirmed, four of which constituted new records for the Mexican Pacific, as well as three harmful species, one toxic <span>and two bloom-forming. Our molecular results evidenced the need to re-evaluate</span> the current taxonomic system of athecate dinoflagellates at all hierarchical levels, integrating them with morphological evidence without preponderance of this criterion over taxonomic decisions.

关 键 词：Diversity HABS New Records Paraphyletic Groups Athecate Dinoflagellates 

分 类 号：O17[理学—数学]