Identification of Rhizobia Isolated from Nodules of Mexican Commercial Soybean Varieties  

作　　者：Cecilia Vázquez Rodríguez Lourdes Vital López Jesús Gerardo García Olivares Homar Rene Gill Langarica Cecilia Vázquez Rodríguez;Lourdes Vital López;Jesús Gerardo García Olivares;Homar Rene Gill Langarica(Laboratorio de Biotecnología Vegetal, Centro de Biotecnología Genómica, Instituto Politécnico Nacional, Reynosa, México;Carrera de Mantenimiento Industrial, Universidad Tecnológica de Tamaulipas Norte, Universidad Tecnológica, Reynosa, México)

机构地区：[1]Laboratorio de Biotecnología Vegetal, Centro de Biotecnología Genómica, Instituto Politécnico Nacional, Reynosa, México [2]Carrera de Mantenimiento Industrial, Universidad Tecnológica de Tamaulipas Norte, Universidad Tecnológica, Reynosa, México

出　　处：《American Journal of Plant Sciences》2024年第1期29-45,共17页美国植物学期刊（英文）

摘　　要：Rhizobia, crucial for nitrogen fixation in leguminous plants, play a vital role in soybean cultivation. This study, conducted in Mexico, a major soybean importer, aimed to identify bacteria from nodules of five soybean varieties in high-production regions. Multilocus sequence analysis (MLSA) was employed for enhanced species resolution. The study identified six Bradyrhizobium species: Bradyrhizobium japonicum USDA 110, Bradyrhizobium japonicum USDA 6, Bradyrhizobium elkanii USDA 76, Bradyrhizobium neotropicale, Bradyrhizobium lablabi, and Bradyrhizobium icense. Bradyrhizobium japonicum USDA 110 predominated in the soils, displaying symbiotic preference for the Huasteca 400 variety. However, phylogenetic analysis didn't reveal a clear association between strains, soil, and soybean variety. This research sheds light on the diversity of rhizobia in Mexican soybean cultivation, contributing to the understanding of symbiotic relationships in soybean production systems.Rhizobia, crucial for nitrogen fixation in leguminous plants, play a vital role in soybean cultivation. This study, conducted in Mexico, a major soybean importer, aimed to identify bacteria from nodules of five soybean varieties in high-production regions. Multilocus sequence analysis (MLSA) was employed for enhanced species resolution. The study identified six Bradyrhizobium species: Bradyrhizobium japonicum USDA 110, Bradyrhizobium japonicum USDA 6, Bradyrhizobium elkanii USDA 76, Bradyrhizobium neotropicale, Bradyrhizobium lablabi, and Bradyrhizobium icense. Bradyrhizobium japonicum USDA 110 predominated in the soils, displaying symbiotic preference for the Huasteca 400 variety. However, phylogenetic analysis didn't reveal a clear association between strains, soil, and soybean variety. This research sheds light on the diversity of rhizobia in Mexican soybean cultivation, contributing to the understanding of symbiotic relationships in soybean production systems.

关 键 词：NODULES Soybean Housekeeping Genes MLSA RHIZOBIA BRADYRHIZOBIUM Nitrogen Fixation SYMBIOSIS Phylogenetic Analysis 

分 类 号：S15[农业科学—土壤学]