Genetic Diversity of Jute Mallow (Corchorus spp.) Accessions Based on ISSR Markers  

作　　者：Munguatosha Ngomuo Tsvetelina Stoilova Tileye Feyissa Patrick A. Ndakidemi Munguatosha Ngomuo;Tsvetelina Stoilova;Tileye Feyissa;Patrick A. Ndakidemi(School of Life Sciences, Nelson Mandela African Institute of Science and Technology, Arusha, Tanzania;Tanzania Official Seed Certification Institute (TOSCI), Arusha, Tanzania;AVRDC—,World Vegetable Centre, Arusha, Tanzania;Institute of Biotechnology, Addis Ababa University, Addis Ababa, Ethiopia)

机构地区：[1]School of Life Sciences, Nelson Mandela African Institute of Science and Technology, Arusha, Tanzania [2]Tanzania Official Seed Certification Institute (TOSCI), Arusha, Tanzania [3]AVRDC—,World Vegetable Centre, Arusha, Tanzania [4]Institute of Biotechnology, Addis Ababa University, Addis Ababa, Ethiopia

出　　处：《American Journal of Plant Sciences》2024年第5期316-328,共13页美国植物学期刊（英文）

摘　　要：Jute mallow is a nutritious leafy vegetable. The leaves are rich in proteins, vitamins and essential amino acids. Molecular characterization of Jute mallow with focus on improvement of leaf yield is scarcely reported. In the present study, inter sequence simple repeats (ISSR) molecular markers were employed to assess genetic diversity and relationships of 83 accessions of Jute mallow from different parts of Africa and Asia conserved at the World Vegetable Center East and Southern Africa. A total of 89 bands were amplified by 8 ISSR primers. Number of polymorphic bands per primer ranged from 2 to 6 with an average of 2.75 bands per primer. Polymorphic information content (PIC) values ranged from 0.390 to 0.760 with average of 0.53. Average Nei’s gene diversity (h) and Shannon’s information index (I) were 0.335 and 0.494 respectively. The highest pairwise genetic distance was 0.431 observed in a population from East Africa accessions. PC1 and PC2 axis explained 21.69% and 11.66% of the total variation respectively. UPGMA cluster analysis grouped the accessions into six main clusters at genetic similarity coefficient of 0.53 as standard value for classification. These results have important implications for jute mallow breeding and conservation.Jute mallow is a nutritious leafy vegetable. The leaves are rich in proteins, vitamins and essential amino acids. Molecular characterization of Jute mallow with focus on improvement of leaf yield is scarcely reported. In the present study, inter sequence simple repeats (ISSR) molecular markers were employed to assess genetic diversity and relationships of 83 accessions of Jute mallow from different parts of Africa and Asia conserved at the World Vegetable Center East and Southern Africa. A total of 89 bands were amplified by 8 ISSR primers. Number of polymorphic bands per primer ranged from 2 to 6 with an average of 2.75 bands per primer. Polymorphic information content (PIC) values ranged from 0.390 to 0.760 with average of 0.53. Average Nei’s gene diversity (h) and Shannon’s information index (I) were 0.335 and 0.494 respectively. The highest pairwise genetic distance was 0.431 observed in a population from East Africa accessions. PC1 and PC2 axis explained 21.69% and 11.66% of the total variation respectively. UPGMA cluster analysis grouped the accessions into six main clusters at genetic similarity coefficient of 0.53 as standard value for classification. These results have important implications for jute mallow breeding and conservation.

关 键 词：Corchorus spp. Genetic Diversity ISSRS Jute Mallow Leafy Vegetable 

分 类 号：S51[农业科学—作物学]