Antimicrobial Activities of Plant Extracts against Streptococcus pneumoniae Isolated from Pediatric Patients at Federal Teaching Hospital Abakaliki, Ebonyi State  

作　　者：Euslar Nnenna Onu Donatus Chukwuma Ilang Cornelius Ituma Azi Theophilus Onwe Eze Chinedu Obasi Akpa Oluwatoyin Oluwatosin Kelechi-Mathew Okechukwu Emmanuel Ogbonna Samuel Eleojo Agada Euslar Nnenna Onu;Donatus Chukwuma Ilang;Cornelius Ituma Azi;Theophilus Onwe Eze;Chinedu Obasi Akpa;Oluwatoyin Oluwatosin Kelechi-Mathew;Okechukwu Emmanuel Ogbonna;Samuel Eleojo Agada(Department of Microbiology, Faculty of Biological Sciences, Alex-Ekwueme Federal University Ndufu-Alike, Ikwo, Ebonyi State, Nigeria;Northern Care Alliance NHS Foundation Trust, Manchester, United Kingdom;Park Hospital Burntstump Country Park, Nottingham, United Kingdom;Department of Haematology, Faculty of Basic Clinical Sciences, Alex-Ekwueme Federal University Ndufu-Alike, Ikwo, Abakaliki, Nigeria;Department of Stems and Regenerative Medicine, University of Chester, Chester, United Kingdom;Department of Biotechnology, University of Chester, Chester, United Kingdom)

机构地区：[1]Department of Microbiology, Faculty of Biological Sciences, Alex-Ekwueme Federal University Ndufu-Alike, Ikwo, Ebonyi State, Nigeria [2]Northern Care Alliance NHS Foundation Trust, Manchester, United Kingdom [3]Park Hospital Burntstump Country Park, Nottingham, United Kingdom [4]Department of Haematology, Faculty of Basic Clinical Sciences, Alex-Ekwueme Federal University Ndufu-Alike, Ikwo, Abakaliki, Nigeria [5]Department of Stems and Regenerative Medicine, University of Chester, Chester, United Kingdom [6]Department of Biotechnology, University of Chester, Chester, United Kingdom

出　　处：《American Journal of Plant Sciences》2024年第10期847-857,共11页美国植物学期刊（英文）

摘　　要：Antibiotic-resistant genes have become a threat to synthetic or conventional medications and because of this much work has been done on using plants and plants part to treat disease caused by bacteria, Herbal medicine has served as effective treatment against various diseases caused by pathogenic bacteria and multi drug-resistant strains of bacteria which made it advantageous over synthetic medications. This study aimed to reveal the sensitivity of Streptococcus pneumoniae from clinical isolate and perform antibacterial assay on the organism using plants leaf extracts of Ocimum gratissimum, Sida acuta, Newbouldia laevia and Mimosa pudica. Gram staining and various biochemical test were used for the identification of Streptococcus pneumoniae. The plants leaves were aseptically washed, dried and ground into fine powder and diluted in varying concentration and agar well diffusion method was used to test for the antimicrobial properties of this plants on Streptococcus pneumoniae at various concentrations as follows 0.1 g/ml, 0.4 g/ml, 0.6 g/ml and 1 g/ml. The plants extract of Ocimum gratissimum showed a greater antibacterial effects on Streptococcus pneumoniae in high concentration more than other plant extracts while Sida acuta and Newbouldia laevia plant extract showed weak antibacterial properties to the organism. This proves that Ocimum gratissimum and Mimosa pudica leaves have good and strong antibacterial properties against Streptococcus pneumoniae than Sida acuta and Newbouldia laevia and can be used as antibacterial agent at adequate concentrations.Antibiotic-resistant genes have become a threat to synthetic or conventional medications and because of this much work has been done on using plants and plants part to treat disease caused by bacteria, Herbal medicine has served as effective treatment against various diseases caused by pathogenic bacteria and multi drug-resistant strains of bacteria which made it advantageous over synthetic medications. This study aimed to reveal the sensitivity of Streptococcus pneumoniae from clinical isolate and perform antibacterial assay on the organism using plants leaf extracts of Ocimum gratissimum, Sida acuta, Newbouldia laevia and Mimosa pudica. Gram staining and various biochemical test were used for the identification of Streptococcus pneumoniae. The plants leaves were aseptically washed, dried and ground into fine powder and diluted in varying concentration and agar well diffusion method was used to test for the antimicrobial properties of this plants on Streptococcus pneumoniae at various concentrations as follows 0.1 g/ml, 0.4 g/ml, 0.6 g/ml and 1 g/ml. The plants extract of Ocimum gratissimum showed a greater antibacterial effects on Streptococcus pneumoniae in high concentration more than other plant extracts while Sida acuta and Newbouldia laevia plant extract showed weak antibacterial properties to the organism. This proves that Ocimum gratissimum and Mimosa pudica leaves have good and strong antibacterial properties against Streptococcus pneumoniae than Sida acuta and Newbouldia laevia and can be used as antibacterial agent at adequate concentrations.

关 键 词：CONVENTIONAL HERBAL ANTIBACTERIAL EXTRACTS CONCENTRATIONS 

分 类 号：R28[医药卫生—中药学]