Enhancing Coating Inoculation for Improved Nitrogen-Fixing Efficiency of Rhizobia in Soybean (Glycine max L.)  

作　　者：Anne Nora Ndokon Bikang Liliane Meguekam Tekam Alain-Martial Sontsa-Donhoung Dieudonné Nwaga Anne Nora Ndokon Bikang;Liliane Meguekam Tekam;Alain-Martial Sontsa-Donhoung;Dieudonné Nwaga(Soil Microbiology Laboratory, Biotechnology Centre, University of Yaounde I, Yaounde, Cameroon;Department of Biological Sciences, Higher Teacher Training College of Yaounde, University of Yaounde I, Yaounde, Cameroon;Laboratory of Regional Biological Control and Applied Microbiology, Institute of Agricultural Research for Development (IRAD), Yaounde, Cameroon;GIC AGRIBIOCAM, Yaounde, Cameroon)

机构地区：[1]Soil Microbiology Laboratory, Biotechnology Centre, University of Yaounde I, Yaounde, Cameroon [2]Department of Biological Sciences, Higher Teacher Training College of Yaounde, University of Yaounde I, Yaounde, Cameroon [3]Laboratory of Regional Biological Control and Applied Microbiology, Institute of Agricultural Research for Development (IRAD), Yaounde, Cameroon [4]GIC AGRIBIOCAM, Yaounde, Cameroon

出　　处：《American Journal of Plant Sciences》2025年第2期245-262,共18页美国植物学期刊(英文)

摘　　要：Plant biofertilization involves introducing compounds containing living mi-croorganisms into the coating medium to sustainably enhance plant production and soil health. This is a complex process that undergoes multiple stages of development before yielding a final product. The final biofertilizer is used by legumes-protein-rich crops in symbiosis with rhizobia to enable biological nitrogen fixation increasing natural soil fertility. This study aims to determine the optimal formulation of a rhizobial biofertilizer to improve the performance of soybean (Glycine max L. cv. Docko). To this end, soybean seeds obtained from IRAD were coated with different formulations derived from locally sourced materials. Palm kernel oil was used as an adhesive in one group, while corn powder served as an adhesive in another. The coated seeds were then sown in the field. The results indicate that the combination of pigeon pea powder + sugarcane molasses, with palm kernel oil as an adhesive, produced the best nodulation (nitrogen fixation). This formulation also led to significant improvements in growth (+350%) and total nitrogen content (+1100%) compared to the bacterial broth inoculum control (B0) (P ≤ 0.01). These findings represent a significant advancement in improving nitrogen-fixing bacterial inoculants and enhancing soil fertility for the sustainable cultivation of soybeans in this tropical soil.Plant biofertilization involves introducing compounds containing living mi-croorganisms into the coating medium to sustainably enhance plant production and soil health. This is a complex process that undergoes multiple stages of development before yielding a final product. The final biofertilizer is used by legumes-protein-rich crops in symbiosis with rhizobia to enable biological nitrogen fixation increasing natural soil fertility. This study aims to determine the optimal formulation of a rhizobial biofertilizer to improve the performance of soybean (Glycine max L. cv. Docko). To this end, soybean seeds obtained from IRAD were coated with different formulations derived from locally sourced materials. Palm kernel oil was used as an adhesive in one group, while corn powder served as an adhesive in another. The coated seeds were then sown in the field. The results indicate that the combination of pigeon pea powder + sugarcane molasses, with palm kernel oil as an adhesive, produced the best nodulation (nitrogen fixation). This formulation also led to significant improvements in growth (+350%) and total nitrogen content (+1100%) compared to the bacterial broth inoculum control (B0) (P ≤ 0.01). These findings represent a significant advancement in improving nitrogen-fixing bacterial inoculants and enhancing soil fertility for the sustainable cultivation of soybeans in this tropical soil.

关 键 词：FORMULATION BIOFERTILIZER N-Fixing RHIZOBIA COATING Growth 

分 类 号：TG1[金属学及工艺—金属学]