Hepatitis A Virus PCR Analysis and <i>E. coli</i>Detection in Oysters at Oualidia Lagoon and Their Correlation  

作　　者：Naima El Moqri Najwa Hassou Fatiha El Mellouli Hasnae Zekhnini Nabil Abouchoaib Samira Etahiri Naima El Moqri;Najwa Hassou;Fatiha El Mellouli;Hasnae Zekhnini;Nabil Abouchoaib;Samira Etahiri(Marine Biotechnology and Environment Laboratory, Faculty of Sciences El Jadida, Chouaib Doukkali University, El Jadida, Morocco;Casablanca Regional Research and Analysis Laboratory, National Office of Food Safety (ONSSA), Casablanca, Morocco)

机构地区：[1]Marine Biotechnology and Environment Laboratory, Faculty of Sciences El Jadida, Chouaib Doukkali University, El Jadida, Morocco [2]Casablanca Regional Research and Analysis Laboratory, National Office of Food Safety (ONSSA), Casablanca, Morocco

出　　处：《Food and Nutrition Sciences》2020年第7期684-694,共11页食品与营养科学（英文）

摘　　要：The present study aims to evaluate hepatitis A virus (HAV) prevalence and faecal contamination indicators <span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">Escherichia coli</span></i><i><span style="font-family:Verdana;"></span></i></i><span style="font-family:Verdana;"> (</span><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">E. coli</span></i><i><span style="font-family:Verdana;"></span></i></i><span style="font-family:Verdana;">) in oysters from Oualidia lagoon (Moroccan Atlantic coast) and to study the correlation between the two parameters. The survey was carried out on 87 samples of oysters (</span><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">Crassostrea gigas</span></i><i><span style="font-family:Verdana;"></span></i></i><span style="font-family:Verdana;">) collected monthly between November 2015 and February 2017 from three sites corresponding to different oyster farms in the lagoon. Sanitary status of bivalve molluscs was assessed by </span><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">E. coli</span></i><i><span style="font-family:Verdana;"></span></i></i><span style="font-family:Verdana;"> enumeration using ISO 16649-3. Detection of hepatitis A virus, was carried out by real-time reverse transcriptase polymerase chain reaction (rRT-PCR) according to ISO 15216-2 method. The prevalence of samples for which </span><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">E. coli</span></i><i><span style="font-family:Verdana;"></span></i></i><span style="font-family:Verdana;"> contamination exceeds the threshold of 230 </span><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">E. coli</span></i><i><span style="font-family:Verdana;"></span></i></i><span style="font-family:Verdana;">/100g of flesh and intravalvular fluid (FIF) is 43%. HAV RNA was detected in 2% of the samplThe present study aims to evaluate hepatitis A virus (HAV) prevalence and faecal contamination indicators <span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">Escherichia coli</span></i><i><span style="font-family:Verdana;"></span></i></i><span style="font-family:Verdana;"> (</span><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">E. coli</span></i><i><span style="font-family:Verdana;"></span></i></i><span style="font-family:Verdana;">) in oysters from Oualidia lagoon (Moroccan Atlantic coast) and to study the correlation between the two parameters. The survey was carried out on 87 samples of oysters (</span><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">Crassostrea gigas</span></i><i><span style="font-family:Verdana;"></span></i></i><span style="font-family:Verdana;">) collected monthly between November 2015 and February 2017 from three sites corresponding to different oyster farms in the lagoon. Sanitary status of bivalve molluscs was assessed by </span><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">E. coli</span></i><i><span style="font-family:Verdana;"></span></i></i><span style="font-family:Verdana;"> enumeration using ISO 16649-3. Detection of hepatitis A virus, was carried out by real-time reverse transcriptase polymerase chain reaction (rRT-PCR) according to ISO 15216-2 method. The prevalence of samples for which </span><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">E. coli</span></i><i><span style="font-family:Verdana;"></span></i></i><span style="font-family:Verdana;"> contamination exceeds the threshold of 230 </span><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">E. coli</span></i><i><span style="font-family:Verdana;"></span></i></i><span style="font-family:Verdana;">/100g of flesh and intravalvular fluid (FIF) is 43%. HAV RNA was detected in 2% of the sampl

关 键 词：Hepatitis A Virus E. coli Bivalves SHELLFISH RT-PCR Oualidia Lagoon 

分 类 号：O17[理学—数学]