Detection of Campylobacter sp. from Poultry Feces in Ouagadougou, Burkina Faso  

作　　者：Assèta Kagambèga Alexandre Thibodeau Daniel K. Soro Nicolas Barro Philippe Fravalo Assèta Kagambèga;Alexandre Thibodeau;Daniel K. Soro;Nicolas Barro;Philippe Fravalo(Laboratoire de Biologie Moléculaire, d’épidémiologie et de surveillance des bactéries et virus transmissibles par les aliments (LaBESTA)/Ecole Doctorale Sciences et Technologies (EDST)/Université Joseph KI-ZERBO,Ouagadougou, Burkina Faso;Institut Des Sciences (IDS), Ouagadougou, Burkina Faso;Veterinary Medicine Faculty, Department of Pathology and Microbiology, NSERC Industrial Research Chair in Meat-Safety (CRSV), University of Montreal, Saint-Hyacinthe, QC, Canada)

机构地区：[1]Laboratoire de Biologie Moléculaire, d’épidémiologie et de surveillance des bactéries et virus transmissibles par les aliments (LaBESTA)/Ecole Doctorale Sciences et Technologies (EDST)/Université Joseph KI-ZERBO,Ouagadougou, Burkina Faso [2]Institut Des Sciences (IDS), Ouagadougou, Burkina Faso [3]Veterinary Medicine Faculty, Department of Pathology and Microbiology, NSERC Industrial Research Chair in Meat-Safety (CRSV), University of Montreal, Saint-Hyacinthe, QC, Canada

出　　处：《Food and Nutrition Sciences》2021年第2期107-114,共8页食品与营养科学（英文）

摘　　要：<b><span style="font-family:Verdana;">Background:</span></b><span style="font-family:""> <i><span style="font-family:Verdana;">Campylobacter</span></i><span style="font-family:Verdana;"> contamination in poultry and poultry product has been reported worldwide. The present study aims to determine the prevalence of </span><i><span style="font-family:Verdana;">Campylobacter</span></i><span style="font-family:Verdana;"> in poultry feces using selective enrichment Bolton broth and multiplex PCR. </span><b><span style="font-family:Verdana;">Method:</span></b><span style="font-family:Verdana;"> Two methods were used in this study</span></span><span style="font-family:Verdana;">:</span><span style="font-family:Verdana;"> the first </span><span style="font-family:Verdana;">was</span><span style="font-family:""> <span style="font-family:Verdana;">direct</span><span style="font-family:Verdana;"> plating of poultry feces into mCCDA agar plates. </span><span style="font-family:Verdana;">The second</span><span style="font-family:Verdana;">, three</span><span style="font-family:Verdana;"> antibiotics were used at different concentrations to add in Bolton broth supplemented. These antibiotics were Rifampicin (Oxoid, </span><span style="font-family:Verdana;">Nepean, Ontario) with 10</span></span><span style="font-family:""> </span><span style="font-family:Verdana;">mg/L, colistin (Oxoid, Nepean, Ontario) with 1</span><span style="font-family:""> </span><span style="font-family:Verdana;">mg/mL and 2</span><span style="font-family:""> </span><span style="font-family:Verdana;">mg/mL;trimethoprim (Oxoid, Nepean, Ontario) with 10</span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">mg/L. The colonies with typical </span><i><span style="font-family:Verdana;">campylobacter</span></i><span style="font-family:Verdana;"> morphology on blood agar (little, red </span><span style="font-family:Verdana;">and</span><span style="font-family:Verdana;"> ring colonies) were further identified to th<b><span style="font-family:Verdana;">Background:</span></b><span style="font-family:""> <i><span style="font-family:Verdana;">Campylobacter</span></i><span style="font-family:Verdana;"> contamination in poultry and poultry product has been reported worldwide. The present study aims to determine the prevalence of </span><i><span style="font-family:Verdana;">Campylobacter</span></i><span style="font-family:Verdana;"> in poultry feces using selective enrichment Bolton broth and multiplex PCR. </span><b><span style="font-family:Verdana;">Method:</span></b><span style="font-family:Verdana;"> Two methods were used in this study</span></span><span style="font-family:Verdana;">:</span><span style="font-family:Verdana;"> the first </span><span style="font-family:Verdana;">was</span><span style="font-family:""> <span style="font-family:Verdana;">direct</span><span style="font-family:Verdana;"> plating of poultry feces into mCCDA agar plates. </span><span style="font-family:Verdana;">The second</span><span style="font-family:Verdana;">, three</span><span style="font-family:Verdana;"> antibiotics were used at different concentrations to add in Bolton broth supplemented. These antibiotics were Rifampicin (Oxoid, </span><span style="font-family:Verdana;">Nepean, Ontario) with 10</span></span><span style="font-family:""> </span><span style="font-family:Verdana;">mg/L, colistin (Oxoid, Nepean, Ontario) with 1</span><span style="font-family:""> </span><span style="font-family:Verdana;">mg/mL and 2</span><span style="font-family:""> </span><span style="font-family:Verdana;">mg/mL;trimethoprim (Oxoid, Nepean, Ontario) with 10</span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">mg/L. The colonies with typical </span><i><span style="font-family:Verdana;">campylobacter</span></i><span style="font-family:Verdana;"> morphology on blood agar (little, red </span><span style="font-family:Verdana;">and</span><span style="font-family:Verdana;"> ring colonies) were further identified to th

关 键 词：CAMPYLOBACTER Poultry Feces Selective Method Multiplex PCR 

分 类 号：O17[理学—数学]